Tip-2 diyabetli hastalarda NFKB1 polimorfizmleri ile telomer uzunluğu ve apoptoz arasındaki ilişkinin araştırılması

Year 2023, Volume: 48 Issue: 1, 216 - 226, 31.03.2023

Ebubekir Dirican , Yasemin Kaya

https://doi.org/10.17826/cumj.1238482

Abstract

Amaç: Tip 2 diabetes mellitus (T2DM), dünya nüfusunun büyük bir bölümünü etkileyen kronik, metabolik ve heterojen bir hastalıktır. Bu çalışma, T2DM'li hastalarda NFKB1 -94 ATTG ins/del polimorfizmlerinin, apoptoz genlerinin ekspresyonu ve telomer uzunluğunu (TU) sağlıklı bireylerle karşılaştırmalı olarak değerlendirmeyi amaçladı.
Gereç ve Yöntem: Çalışmaya 69 T2DM hastası ve 60 sağlıklı kişi dahil edildi. Kan örneklerinden DNA ve RNA izole edildi. NFKB1 genotipleri, Sanger sekansı ile tanımlandı. TU analizleri için ve kaspaz-3, kaspaz-9, bax ve bcl2 genlerinin ekspresyonunu araştırmak için RT-PCR kullanıldı.
Bulgular: NFKB1 -94 ins/del genotipli hastalar ile kontrol grubu arasında anlamlı bir fark vardı (OR:0,4792 (0,2345-1,011)). Bununla birlikte, diğer genotip/alellerin (ins/ins ve del/del) dağılımı, T2DM ve kontrol grupları arasında fark göstermedi. NFKB1-94 ins/del'in alelik frekansı T2DM grubu için 0,455/0,235 ve kontrol grubu için 0,435/0,165’idi. T2DM grubunda kaspaz-3, kaspaz-9 ve Bax genlerinin mRNA ekspresyonunda sağlıklı gruba göre artış gözlenirken, T2DM grubunda Bcl2 geninde azalma saptandı. T2DM hastalarında TU, sağlıklı bireylerden daha kısaydı.
Sonuç: NFKB1 -94 ins/del polimorfizmleri, T2DM hastalarında anlamlı farklılık göstermektedir. T2DM'li hastalarda apoptozun aktive olduğunu ve TU'nun kısaldığını gözlemledik. Ancak NFKB1 polimorfizmleri ile apoptoz ve TU arasında ilişki saptanamadı.

Keywords

T2DM, NFKB1, apoptoz, telomere uzunluğu, Sanger sekans

Supporting Institution

Bayburt Üniversitesi Bilimsel Araştırma Proje Koordinatörlüğü tarafından desteklenmiştir.

Project Number

BAU-BAP- No: 2021/69001-01-10

Investigation of the relationship between NFKB1 polymorphisms and telomere length and apoptosis in patients with type-2 diabetes

Abstract

Purpose: Type 2 diabetes mellitus (T2DM) is a heterogeneous, chronic, and metabolic disease that affects a significant proportion of the global population. This study aimed to evaluate the effect of NFKB1 -94 ATTG ins/del polymorphisms on the expression of apoptosis genes and telomere length (TL) in patients with T2DM compared with healthy individuals.
Materials and Methods: Sixty-nine T2DM patients and sixty healthy people were enrolled in the study. DNA and RNA were isolated from the blood samples. NFKB1 genotypes were identified by Sanger sequencing. For TL analyses and to investigate the expression of the caspase-3, caspase-9, bax, and bcl2 genes, RT-PCR was utilized.
Results: There was a significant difference between the NFKB1 -94 ins/del genotype patients and the control group (OR:0.4792 (0.2345-1.011)). However, the distribution of other genotype/alleles (ins/ins and del/del) showed no difference between T2DM and control groups. The allelic frequency of NFKB1 -94 ins/del was 0.455/0.235 for the T2DM group and 0.435/0.165 for the control group. An increase in the mRNA expression of caspase-3, caspase-9 and Bax genes was observed in the T2DM group compared with the healthy group, while a decrease in the Bcl2 gene was found in the T2DM group. TL in T2DM patients was shorter than in healthy individuals.
Conclusion: NFKB1 -94 ins/del polymorphisms show significant differences in T2DM patients. We observed that apoptosis was activated and TL was shortened in patients with T2DM. However, no relationship between NFKB1 polymorphisms and apoptosis and TL could not be determined.

Keywords

T2DM, NFKB1, apoptosis, telomere length, Sanger sequencing

Project Number

BAU-BAP- No: 2021/69001-01-10

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