Association of Kruppel-like factor 2 polymorphisms with the risk of coronary artery disease

Year 2023, Volume: 48 Issue: 2, 583 - 592, 02.07.2023

Ulaş Değirmenci , Metin Yıldırım , Merih Akkapulu , Ali Yalın , Dilek çiçek Dilek çiçek , Ragıp Onur Öztornacı , Bahar Taşdelen , Serap Yalın

https://doi.org/10.17826/cumj.1283236

Abstract

Purpose: Coronary artery disease is defined as complications that develop in proportion to the prevalence of ischemia due to occlusion of the coronary arteries and the resulting cell death. The development of atherosclerosis is significantly influenced by endothelial cell dysfunction. Kruppel-like factor 2, a transcription factor, has been shown to regulate critical biological events in endothelial biology, such as vascular tone, migration, proliferation, vasoreactivity, and angiogenesis. In our study, it was aimed to clarify the relationship between coronary artery disease and Kruppel-like factor 2 protein levels and C1239A polymorphisms.
Materials and Methods: 191 individuals who underwent coronary angiography at Mersin University Faculty of Medicine, Department of Cardiology, Health, Research and Application Center were included in the study. Measurements of serum fasting blood glucose, HDL cholesterol, total cholesterol, and triglyceride levels were performed on the AU5800 (Beckman Coulter, United States) autoanalyzer. Serum LDL levels were calculated using the Friedwald equation. Serum Kruppel-like factor 2 protein levels were measured by sandwich enzyme-linked immunoassay method on the Multiskan GO (Thermo Scientific, Finland) device. Kruppel-like factor 2 C1239A variations were detected on the Applied Biosystem VIIA™ 7 Real-Time PCR (Life Technologies Co., United States) device by TaqMan® single nucleotide polymorphism (SNP) genotyping method.
Results: Men had a 3.8-fold higher risk of CAD than women. (Odd’s ratio 3.83, 95% Confidence interval 1.98-7.39; p<0.001). Kruppel-Like Factor 2 protein levels were not significantly different amongst the groups. Kruppel-like factor 2 C1239A polymorphisms were not associated with coronary artery disease.
Conclusion: More comprehensive studies with larger sample groups including other gene regions and meta-analysis studies are needed to determine the relationship between Kruppel-like factor 2 gene polymorphisms and coronary artery disease.

Keywords

Coronary artery disease, Atheroseclerosis, Endothel, Kruppel-like factor 2, Single nucleotide polymorphism

Supporting Institution

Mersin Üniversitesi Bilimsel Araştırma Projeleri Birimi

Project Number

2018-2 TP3-2983

Thanks

This research was supported by the Research Foundation of Mersin University, Mersin, Turkey (Grant no. 2018-2 TP3-2983)

Kruppel benzeri faktör 2 polimorfizmlerinin koroner arter hastalığı riski ile ilişkisi

Abstract

Amaç: Koroner arter hastalığ, koroner arterlerin tıkanmasıyla iskemi oluşması ve sonucunda gerçekleşen hücre ölümlerinin yaygınlığıyla orantılı gelişen komplikasyonlar olarak tanımlanır. Ateroskleroz oluşumunda endotel hücrelerinin disfonksiyonu önemli rol oynamaktadır. Bir transkripsiyon faktörü olan Kruppel benzeri faktör 2’nin endotel biyolojisinde vasküler tonus, migrasyon, proliferasyon, vazoreaktivite ve anjiyogenez gibi önemli biyolojik olayları düzenlediği ortaya konulmuştur. Çalışmamızda Kruppel benzeri faktör 2 protein düzeyleri ve C1239A polimorfizmlerinin koroner arter hastalığıyla ilişkisinin aydınlatılması amaçlandı.
Gereç ve Yöntem: Mersin Üniversitesi Tıp Fakültesi Hastanesi Kardiyoloji Polikliniğinde koroner anjiyografi yapılan 191 birey çalışmaya dahil edildi. Serum açlık kan şekeri, total kolesterol, HDL kolesterol ve trigliserit düzeylerinin ölçümleri AU5800 (Beckman Coulter, Amerika Birleşik Devletleri) otoanalizöründe yapıldı. Serum LDL düzeyleri Friedwald eşitliği kullanılarak hesaplandı. Serum Kruppel benzeri faktör 2 protein düzeyleri sandviç enzim bağlı immünoassay yöntemiyle Multiskan GO (Thermo Scientific, Finlandiya) cihazında çalışıldı. Kruppel benzeri faktör 2 C1239A varyasyonları TaqMan® tek nükleotid polimorfizm (SNP) genotiplendirme yöntemiyle Applied Biyosistem VIIA ™ 7 Real-Time PCR (Life Technologies Co., Amerika Birleşik Devletleri) cihazında saptandı.
Bulgular: Erkeklerin kadınlara göre KAH açısından 3,8 kat daha riskli olduğu saptandı (Odd’s Oranı 3,83, %95 Güven Aralığı 1,98-7,39; p<0,001). Kruppel Benzeri Faktör 2 protein düzeylerinin gruplar arasında anlamlı farklılık göstermediği saptandı. Kruppel benzeri faktör 2 C1239A polimorfizmlerinin koroner arter hastalığı için risk teşkil etmediği saptandı.
Sonuç: Kruppel benzeri faktör 2 gen polimorfizmlerinin KAH için risk faktörü olup olmadığının ortaya konulması için diğer gen bölgelerini de içeren daha geniş örneklem gruplarıyla yapılacak daha kapsamlı çalışmalara ve meta analiz çalımalarına ihtiyaç duyulduğu düşünülmektedir.

Keywords

Koroner arter hastalığı, ateroskleroz, endotel, kruppel benzeri faktör 2, tek nükleotid polimorfizmi

Project Number

2018-2 TP3-2983

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