Transkraniyal doğru akım stimülasyonunun deneysel hafif travmatik beyin hasarının neden olduğu motor fonksiyon kaybı üzerine terapötik etkileri

Year 2023, Volume: 48 Issue: 3, 972 - 978, 30.09.2023

Güven Akçay , Recep Baydemir

https://doi.org/10.17826/cumj.1337529

Cited By: 1

Abstract

Amaç: Travmatik beyin hasarı (TBH) lokomotor aktivite, öğrenme ve hafıza gibi davranışsal bozukluklara neden olan ciddi bir hastalıktır. Bu çalışmanın amacı, deneysel travmatik beyin hasarı modelinde transkraniyal Doğru Akım Stimülasyonu (tDAS) tedavisinin lokomotor aktivite üzerindeki davranışsal etkilerini araştırmak ve motor korteks dokusunda IL-1β ve IL-18 düzeylerini incelemektir.
Gereç ve Yöntem: 30 adet 3 aylık erkek Wistar albino sıçanlar kullanıldı. TBH modeli Marmarou yöntemi kullanılarak oluşturuldu. Sham ve TBH+tDAS gruplarına TBH'nın 2. saatinde başlanıp 2 gün boyunca günde 0.5 mA 30 dakika anodal tDAS tedavisi uygulandı. Lokomotor aktivite açık alan testinde değerlendirildi. Motor korteks dokusunda IL-1β ve IL-18 düzeyleri ELİSA yöntemi ile ölçüldü.
Bulgular: Lokomotor aktivite sonuçları sham grubuna kıyasla TBH grubunda anlamlı düzeyde azalma görülürken), TBH+tDAS grubunda TBH grubuna kıyasla anlamlı artma görüldü. TBH grubunun motor korteks IL-1β ve IL-18 değerlerinde sham grubuna kıyasla anlamlı artma tespit edilirken, TBH+tDAS grubunda TBH grubuna kıyasla anlamlı bir azalam tespit edildi.
Sonuç: Çalışmamızda, tDAS tedavisinin travmatik beyin hasarına karşı nöroinflamasyon üzerinde terapötik etkiye sahip olduğu gösterildi.

Keywords

Motor fonksiyon, terapötik tedavi, travmatik beyin hasarı, transkraniyal doğru akım stimülasyonu

Project Number

Project number: TIP19001.21.003

Therapeutic effects of transcranial direct current stimulation on loss of motor function caused by experimental mild traumatic brain injury

Abstract

Purpose: Traumatic brain injury (TBI) is a serious illness that causes behavioral disorders such as locomotor activity, learning, and memory. This study aims to investigate the behavioral effects of transcranial Direct Current Stimulation (tDCS) treatment on locomotor activity in an experimental traumatic brain injury model and to investigate the levels of IL-1β and IL-18 in the motor cortex tissue.
Materials and Methods: 30 male 3-month-old Wistar albino rats were used. The TBI model was established using the Marmarou method. 2 hours after TBI, sham and TBI+tDCS groups were treated with 0.5 mA 30 minutes anodal tDCS treatment for 2 days. Locomotor activity was evaluated in open field test. IL-1β and IL-18 levels in motor cortex tissue were measured by the ELISA method..
Results: Compared to the sham group, locomotor activity results showed significant decreases in the TBI group while the TBI+tDCS group showed significant increases compared to the TBI group. There were significant increases in IL-1β and IL-18 values in the motor cortex of the animals in the TBI group compared to the sham group, while there was a significant decrease in the TBI+tDCS group compared to the TBI group.
Conclusion: tDCS treatment was shown to have therapeutic effects on neuroinflammation against traumatic brain injury.

Keywords

Motor Function, Therapeutic Treatment, Traumatic Brain Injury, transcranial Direct Current Stimulation

Supporting Institution

This work was supported by the Coordination Unit of Scientific Research Projects of Erciyes University (Project number: TIP19001.21.003)

Project Number

Project number: TIP19001.21.003

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