Obez Wistar albino sıçanlarda yüksek kalorili diyet ve egzersizin enerji metabolizma parametreleri üzerindeki etkileri

Year 2023, Volume: 48 Issue: 4, 1388 - 1395, 29.12.2023

Selcen Çakır , Yunus Demirtaş

https://doi.org/10.17826/cumj.1365921

Abstract

Amaç: Metabolik hastalıkların artmasıyla birlikte enerji metabolizması çalışmaları da ilerlemiş ve yakın zamanda yeni enerji metabolizması üyeleri tanımlanmıştır. Çalışmanın amacı, yüksek kalorili diyet ve egzersizin yeni tanımlanan ve metabolizma üzerindeki etkileri halen araştırılan asprosin, irisin, nesfatin-1 ve preptin peptidleri üzerindeki etkilerini göstermek ve gelecekteki araştırmalar için katkı sağlamaktır.
Gereç ve Yöntem: Bu çalışmada 24 sıçan kontrol grubu, egzersiz grubu, yüksek kalorili diyet grubu ve yüksek kalorili diyet ve egzersiz grubu olmak üzere 4 gruba ayrıldı. Serum asprosin, irisin preptin, nesfatin-1 insülin düzeyleri ELISA yöntemi ile ölçüldü. Serum glukoz, trigliserit (TG), total kolesterol (TK), yüksek yoğunluklu lipoprotein kolesterol (HDL-C) ve düşük yoğunluklu lipoprotein kolesterol (LDL-C) düzeyleri kolorimetrik yöntemle belirlendi.
Bulgular: Kontrol grubuna kıyasla, vücut kitle indeksi, glukoz, asprosin, TG, TC ve LDL-C seviyeleri önemli ölçüde arttı. Deney grubu ile kontrol grubu arasında serum HDL-C seviyeleri belirgin bir şekilde daha düşüktü. Egzersiz grubunda irisin ve nesfatin-1 seviyeleri önemli ölçüde artarken, LDL-C, TG, TC ve glukoz ve preptin seviyelerinde azalma görüldü.
Sonuç: Yüksek kalorili diyet, olumsuz bir lipid profili ile ilişkilendirildi. Egzersiz, genellikle yağ dokusundan türeyen ve/veya enerji metabolizmasını düzenleyen peptitlerin salınımında değişikliklere neden oldu. Fiziksel aktivite, peptit salınımında ve böylece biyokimyasal dengeyi sürdürmede önemli bir faktör olarak ortaya çıksa da mekanizmayı daha net anlamak için daha fazla araştırmaya ihtiyaç vardır.

Keywords

Asprosin, irisin, nesfatin-1, preptin, egzersiz

Ethical Statement

The study was approved by Çanakkale Onsekiz Mart University Animal Experiments Local Ethics Committee with the decision of ÇOMÜ HADYEK 20.04.2020; 2020/04-08 code number.

Supporting Institution

Çanakkale Onsekiz Mart Üniversitesi

Project Number

THD-2020-3346

Effects of high calorie dietary nutrition and exercise on energy metabolism parameters in obese Wistar albino rats

Abstract

Purpose: With the increasing prevalence of metabolic disorders, studies on energy metabolism have advanced, leading to the recent identification of new members in energy metabolism. This study aims to demonstrate the effects of a high-calorie diet and exercise on the newly identified peptides asprosin, irisin, nesfatin-1, and preptin, whose effects on metabolism are still under investigation, and to provide information for future research.
Materials and Methods: In this study, 24 rats were divided into four groups: control, exercise, high-calorie diet, and high-calorie diet with exercise. Serum levels of asprosin, irisin, preptin, nesfatin-1, and insulin were measured using the ELISA method. Additionally, serum levels of glucose, triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were determined by the colorimetric method.
Results: Compared to the control group, there were significant increases in body mass index, glucose, asprosin, TG, TC, and LDL-C levels. Serum HDL-C levels were notably lower in the experimental group compared to the control group. In the exercise group, irisin and nesfatin-1 levels significantly increased, accompanied by reductions in LDL-C, TG, TC, glucose, and preptin levels.
Conclusion: A high-calorie diet was associated with an unfavorable lipid profile, while exercise-induced alterations in the secretion of peptides derived from adipose tissue and/or regulating energy metabolism. Although physical activity emerges as a crucial factor in peptide secretion and maintaining biochemical balance, further research is imperative to comprehensively understand the underlying mechanisms.

Keywords

asprosin, Nesfatin1, Preptin, Irisin, Exercise

Ethical Statement

The study was approved by Çanakkale Onsekiz Mart University Animal Experiments Local Ethics Committee with the decision of ÇOMÜ HADYEK 20.04.2020; 2020/04-08 code number.

Supporting Institution

Çanakkale Onsekiz Mart Üniversitesi

Project Number

THD-2020-3346

References

• Aydin S. Three new players in energy regulation: Preptin, adropin and irisin. Peptides. 2014;56:94–110.
• Pan W, Hsuchou H, Kastin AJ. Nesfatin-1 crosses the blood-brain barrier without saturation. Peptides. 2007;28:2223–8.
• Ozcan S, Ulker N, Bulmus O, Yardimci A, Ozcan M, Canpolat S. The modulatory effects of irisin on asprosin, leptin, glucose levels and lipid profile in healthy and obese male and female rats. Arch Physiol Biochem. 2022;128:724-31.
• Romere C, Duerrschmid C, Bournat J, Constable P, Jain M, Xia F et al. Asprosin, a fasting-induced glucogenic protein hormone. Cell. 2016;21;165:566–79.
• Oh-I S, Shimizu H, Satoh T, Okada S, Adachi S, Inoue K et al. Identification of nesfatin-1 as a satiety molecule in the hypothalamus. Nature. 2006;443:709–12.
• Buchanan CM, Phillips ARJ, Cooper GJS. Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet β-cells and enhances insulin secretion. Biochem J. 2001;360:431–9.
• Boström P, Wu J, Jedrychowski MP, Korde A, Ye L, Lo JC et al. A PGC1-α-dependent myokine that drives brown-fat-like development of white fat and thermogenesis. Nature. 2012;481:463–8.
• Duerrschmid C, He Y, Wang C, Li C, Bournat JC, Romere C et al. Asprosin is a centrally acting orexigenic hormone. Nat Med. 2017;23:1444–53.
• Bu Z, Kuok K, Meng J, Wang R, Xu B, Zhang H. The relationship between polycystic ovary syndrome, glucose tolerance status and serum preptin level. Reprod Biol Endocrinol. 2012;10:10.
• Su Y, Zhang J, Tang Y, Bi F, Liu JN. The novel function of nesfatin-1: Anti-hyperglycemia. Biochem Biophys Res. 2010;391:1039–42.
• Claudio ERG, Endlich PW, Santos RL, Moysés MR, Bissoli NS, Gouvêa SA et al. Effects of chronic swimming training and oestrogen therapy on coronary vascular reactivity and expression of antioxidant enzymes in ovariectomized rats. PLoS One. 2013;8: e64806.
• Zhang L, Chen C, Zhou N, Fu Y, Cheng X. Circulating asprosin concentrations are increased in type 2 diabetes mellitus and independently associated with fasting glucose and triglyceride. Clin Chim Acta 2019;489:183–8.
• Ko JR, Seo DY, Kim TN, Park SH, Kwak HB, Ko KS et al. Aerobic exercise training decreases hepatic asprosin in diabetic rats. J Clin Med. 2019;8:666.
• Mazur-Bialy AI. Asprosin-a fasting-induced, glucogenic, and orexigenic adipokine as a new promising player. Will it be a new factor in the treatment of obesity, diabetes, or infertility? a review of the literature. Nutrients. 2021;13:620.
• Alan M, Gurlek B, Yilmaz A, Aksit M, Aslanipour B, Gulhan I et al. Asprosin: a novel peptide hormone related to insulin resistance in women with polycystic ovary syndrome. Gynecol Endocrinol. 2019;35:220–3.
• Pirani H, Roustaie M, Ravasi AA, Lamir AR. Effects of 8-week high-intensity interval training and continuous aerobic training on asprosin secretion and fibrillin-1 gene expression levels in diabetic male rats. Int J Diabetes Dev Ctries. 2022;42:775–80.
• Hekim MG, Kelestemur MM, Bulmus FG, Bilgin B, Bulut F, Gokdere E et al. Asprosin, a novel glucogenic adipokine: a potential therapeutic implication in diabetes mellitus. Arch Physiol Biochem. 2023;129:1038-44.
• Nakhaei H, Mogharnasi M, Fanaei H. Effect of swimming training on levels of asprosin, lipid profile, glucose and insulin resistance in rats with metabolic syndrome. Obes Med. 2019;1:15.
• Wiecek M, Szymura J, Maciejczyk M, Kantorowicz M, Szygula Z. Acute anaerobic exercise affects the secretion of asprosin, irisin, and other cytokines - A comparison between sexes. Front Physiol. 2018;9:1782.
• Sünnetçi Silistre E, Hatipoğl HU. Increased serum circulating asprosin levels in children with obesity. Pediatr Int. 2020;62:467–76.
• Lu Y, Yuan W, Xiong X, Huang Q, Chen S, Yin T et al. Asprosin aggravates vascular endothelial dysfunction via disturbing mitochondrial dynamics in obesity models. Obesity. 2023;31:732–43.
• Mogharnasi M, TaheriChadorneshin H, Abbasi-Deloei N. Effect of exercise training type on plasma levels of vaspin, nesfatin-1, and high-sensitivity C-reactive protein in overweight and obese women. Obes Med. 2019;13:34–8.
• Nesreen N, Mohsen ES. Moderate exercise training has anorexogenic effect associated with improved oxidative stress in obese women. Int J Nutr Metab. 2015;7:52–61.
• Oguz Ozcelik, Sermin Algul BY. Nesfatin-1 and irisin levels in response to the soccer matches performed in morning, afternoon and at night in young trained male subjects. Cell Mol Biol. 2018;64:4.
• Ghanbari-Niaki A, Hosseini F, Broom DR, Tejenjari B, Rahmati-Ahmadabad S. Combined effects of high-ıntensity aerobic exercise training and ziziphus jujuba extract on tissue nesfatin-1 in rats. Front Endocrinol (Lausanne). 2022;13:845014.
• Roca-Rivada A, Castelao C, Senin LL, Landrove MO, Baltar J, Crujeiras AB et al. FNDC5/Irisin is not only a myokine but also an adipokine. PLoS One. 2013;8:e60563..
• Kang YS, Kim JC, Kim JS, Kim SH. Effects of swimming exercise on serum irisin and bone FNDC5 in rat models of high-fat diet-induced osteoporosis. J Sport Sci Med. 2019;18:596–603.
• Moustafa A, Arisha AH. Swim therapy-induced tissue specific metabolic responses in male rats. Life Sci. 2020;262:118516.
• Vliora M, Grillo E, Corsini M, Ravelli C, Nintou E, Karligiotou E et al. Irisin regulates thermogenesis and lipolysis in 3T3-L1 adipocytes. Biochim Biophys Acta Gen Subj. 2022;1866:130085.
• Erickson HP. Irisin and FNDC5 in retrospect. Adipocyte. 2013;2:289–93.
• Naot D, Cornish J. Cytokines and hormones that contribute to the positive association between fat and bone. Front Endocrinol (Lausanne). 2014;5:70..
• Rasheed NW, Rafidain A. Estimation of preptin, myostatin and some biochemical parameters in diabetic mellitus patients. J Popul Ther Clin Pharmacol. 2023;30:48–55.