Yüksek yağlı diyet ile beslenen ve tam kalınlıkta deri defekti modeli oluşturulan sıçanlarda laktoferrinin etkisi

Year 2023, Volume: 48 Issue: 4, 1396 - 1404, 29.12.2023

Gönül Sena Yıldırım , Ahmet Sarper Bozkurt , Mustafa Örkmez , Hasan Ulusal

https://doi.org/10.17826/cumj.1396328

Abstract

Amaç: Laktoferrin, geniş bir biyoaktivite yelpazesine sahip süt proteini ailesinin bir üyesidir ve birçok mikrop, virüs ve diğer patojenlere karşı tedavi edici etkilere sahiptir. Bu çalışmanın amacı laktoferinin, obeziteye bağlı yara iyileşmesine karşı iyileştirici etkisi göstermesi amaçlanmaktadır.
Gereç ve Yöntem: Bu amaçla 48 adet erkek sıçan Kontrol (C) (n=8), Tam kalınlıkta deri defekti modeli (FT) (n=8), Sığır Laktoferrin (bLf) (n= 8), Yüksek Yağlı Diyet olarak gruplandırıldı. (HFD) + Sığır Laktoferrin (bLf) (n = 8)(200 mg/kg), Yüksek Yağlı Diyet (HFD)+Tam kalınlıkta cilt defekti modeli (FT) (n=8) ve HFD+Tam kalınlıkta cilt defekti modeli (FT) + Sığır Laktoferrin bLf (n=8). Yüksek Yağlı Diyet + bLf ve Yüksek Yağlı Diyet + Tam Kalınlık cilt defekti + bLf grubundaki sıçanlara yüksek yağlı diyet ve sığır laktoferrin oral olarak verildi.IL-6 ve VEGF biyokimyasal parametrelerine serumda bakılmıştır.
Bulgular: Grupların serum IL-6 protein miktarları incelendiğinde 21. günde en yüksek HFD+FT+bLf grubunda, diğer grup olan serum VEGF protein miktarları ise FT grubunda en düşüktü. Biyokimyasal parametreler değerlendirildiğinde bu miktarların 21. günde en yüksek HFD+FT+bLf grubunda, en düşük ise FT grubunda olduğu görüldü.
Sonuç: Laktoferrinin yara iyileşmesini hızlandırdığı gösterilmiştir ve laktoferrinin kolaylıkla bulunabilmesi ve sütte bol miktarda bulunması, onu gelecekte yara iyileşmesi ve iltihaplanma için heyecan verici bir tedavi seçeneği haline getirmektedir.

Keywords

Yara iyileşmesi, Laktoferin, Yüksek yağlı diyet, Sıçan.

Supporting Institution

Gaziantep Üniversitesi Bilimsel araştırma projeleri birimi (Protokol no: TF. YLT.21.31).

Effect of lactoferrin in rats fed with a high-fat diet and with a full-thickness skin defect model

Abstract

Purpose: Lactoferrin is a member of the milk protein family with a broad spectrum of bioactivities and has therapeutic effects against many microbes, viruses, and other pathogens. This study aims to show the healing effect of lactoferrin against obesity-related wound healing.
Materials and Methods: In this study, 48 male rats were grouped as the Control (C) (n =8), Full-thickness skin defect model (FT) (n =8), Bovine Lactoferrin (bLf) (200 mg/kg)(n= 8), High Fat Diet (HFD) + Bovine Lactoferrin (bLf) (n = 8), High-Fat Diet (HFD)+Full-thickness skin defect model (FT) (n=8), and HFD+Full-thickness skin defect model (FT) + Bovine Lactoferrin bLf (n=8). High-Fat Diet + bLf and High-Fat Diet + Full-thickness skin defect + bLf group rats were given a high-fat diet and Bovine Lactoferrin orally. IL-6 and VEGF biochemical parameters were examined in serum.
Results: When the serum IL-6 protein amounts of the groups were examined, it was the highest in the HFD+FT+bLf group on the 21st day, and the lowest in the FT group, when the serum VEGF protein amounts, which were the other biochemical parameters, were evaluated, these amounts were found to be the highest in the HFD+FT+bLf group and the lowest in the FT group on the 21st day.
Conclusion: Lactoferrin has been shown to accelerate wound healing, and the fact that lactoferrin is readily available and abundant in milk makes it an exciting treatment option for wound healing and inflammation in the future.

Keywords

Wound healing, Lactoferrin, Diet, High-Fat, Rats.

Supporting Institution

Gaziantep University Research Project Management Unit for funding. (Grant: TF. YLT.21.31).

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