İçme suyundaki mikroplastiklere maruz kalmanın sperm parametreleri ile TNP1 ve TNP2 sperm nüklear protein genleri üzerindeki etkileri

Year 2024, Volume: 49 Issue: 2, 320 - 331, 30.06.2024

Harika Topal Önal , Tiinçe Aksak , İzzet Oğuz

https://doi.org/10.17826/cumj.1415350

Abstract

Amaç: Mikroplastikler, çevresel kirlilik arttıkça önemli bir endişe kaynağı haline gelmiştir. Ancak, mikroplastiklerin içme suyundaki potansiyel etkileri hala yeterince anlaşılamamıştır. Bu çalışma, plastik şişe ve damacana su tüketen erkekler ile şebeke suyu tüketen erkekler arasındaki içme suyundaki mikroplastiklere maruziyeti araştırmayı amaçlamaktadır.
Gereç ve Yöntem: Çalışmaya toplamda 100 hasta dahil edildi, bunlardan 50'si sadece şebeke suyu kullanırken 50'si plastik şişe ve damacana suyu kullanmaktaydı. Ayrıca, bu çalışma maruziyetin sperm parametreleri üzerindeki etkisini ve sperm nüklear protein genlerinden Transition Protein 1 geni (TNP1) ve TNP2'nin ekspresyon düzeyleri üzerindeki potansiyel etkileri değerlendirdi.
Bulgular: Elde edilen sonuçlara göre, plastik şişe suyu kullananlarda amorf baş anomalisinin, şebeke suyu kullananlara göre belirgin şekilde daha yüksek olduğu saptandı. Ayrıca, plastik şişe kullananlarda bükülmüş kuyruk anomalisi ve kuyruktaki terminal damla anomalisinin de şebeke suyu kullananlara göre anlamlı olarak daha yüksek bulundu. Sonuçlar, içme suyundaki mikroplastiklere maruziyetin erkek sperm parametrelerini etkileyebileceğini ve TNP1 ile TNP2 genlerinin ekspresyonunu değiştirebileceğini göstermektedir.
Sonuç: Çalışma plastik şişe ve damacana su tüketenler ile şebeke suyu tüketenler arasında maruziyet düzeylerinde farklılıklar olduğunu ortaya koymuştur.

Keywords

Mikroplastik, plastik şişe, sperm parametreleri, TNP1, TNP2, gen ekspresyonu

The effects of exposure to microplastics in drinking water on sperm parameters and TNP1 and TNP2 sperm nuclear protein genes

Abstract

Purpose: Microplastics have emerged as a significant concern amid the rise in environmental pollution. Nevertheless, the potential impacts of microplastics present in drinking water on human health remain inadequately understood. This study aims to explore the exposure to microplastics in drinking water among men who consume water from plastic bottles and carboys compared to those who consume tap water.
Materials and Methods: A total of 100 patients were included in the study, 50 of whom used only tap water and 50 of whom used plastic bottles and carboys. In addition, this study evaluated the effects of exposure on sperm parameters and potential effects on the expression levels of the sperm nuclear protein genes transition protein 1 gene (TNP1) and TNP2.
Results: According to the findings, it was observed that the incidence of amorphous headache anomalies among individuals using plastic bottled water was significantly higher compared to those using tap water. In addition, the coiled tail anomaly and the terminal droplet anomaly in the tail were also observed to be significantly higher in people using plastic bottles compared to those using tap water. The results show that exposure to microplastics in drinking water can affect male sperm parameters and alter the expression of TNP1 and TNP2 genes.
Conclusion: It was found that there were differences in exposure levels between those who consumed plastic bottles and carboy water, and those who consumed municipal water.

Keywords

Microplastic;, Plastic bottle, Sperm parametres, TNP1, TNP2, Gene expression

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