Periferik nöropatide subakut dönemde uygulanan mezenkimal kök hücrelerin etkileri

Year 2024, Volume: 49 Issue: 2, 424 - 438, 30.06.2024

Olcay Kıroğlu , Erkan Maytalman , Ares Alizade , Mustafa Emre , Suzan Zorludemir , Fazilet Aksu

https://doi.org/10.17826/cumj.1453906

Abstract

Amaç: Farelerde periferik sinir hasarı oluşturulduktan sonra subakut dönemde uygulanan kemik iliği kökenli mezenkimal kök hücrelerin (Kİ-MKH) 24 haftada nöropatik ağrı ve sinir-kas dokusu fonksiyonları üzerindeki etkilerinin araştırılması amaçlanmıştır.
Gereç ve Yöntem: Farelerde periferik nöropati, siyatik sinir parsiyel ligasyonu yapılarak oluşturuldu. Deneyler Kontrol, Sham, Nöropatik, Kİ-MKH, Nöropatik+Kİ-MKH gruplarında gerçekleştirildi. Allodini, 2.,6. ve 24. haftalarda soğuk plak latens testi ile ölçüldü. 24. haftanın sonunda tüm gruplardan izole edilen sinir-kas dokularında elektrofizyolojik ve histopatolojik incelemeler yapıldı.
Bulgular: Allodini eşiğinin Nöropati+Kİ-MKH grubunda (7.76 ±0.33 sn) nöropati grubuna (4.36±0.21 sn) göre 6. haftadan itibaren arttığı ve 24 hafta boyunca devam ettiği gösterildi. 24. haftada izole edilen sinir dokusunun elektrofizyolojik ölçümleri sonucu, Nöropati+Kİ-MKH grubunun aksiyon potansiyeli (137.9±7.85 mV) ve depolarizasyon (0.74 ± 0.01 ms) değerlerinin nöropati grubuna göre (sırasıyla; 121.5 ±3.03 mV ve 0.81 ± 0.02 ms) kısmi iyileşme gösterdiği belirlendi. Aynı şekilde kas dokusunun istirahat membran potansiyel değerlerinin Nöropati+ Kİ-MKH grubunda nöropati grubuna göre (sırasıyla; -73.4±0.2 ve -87.7±0.2 mV) artmış olduğu ölçüldü. Sinir dokusunun histopatolojik incelemesi sonu nöropati grubunda sinir liflerinin miyelinli aksonlarında kayıp ve endonöryumda belirgin fibrozis, Nöropati+Kİ-MKH grubunda ise Schwann hücre proliferasyonu, sinir liflerinin miyelinli aksonlarının korunduğu görüldü. Kas dokusunun histopatolojik incelemesinde Nöropati grubunda kas lifi atrofisi, kompansatuar hipertrofi lifleri ve artmış iç çekirdekler görülürken, Nöropati+ Kİ-MKH grubunda küçük atrofik kas lifi grupları belirlendi.
Sonuç: Periferik nöropatide, subakut dönemde Kİ-MKH uygulamasının, nöropati grubuna göre hem nöropatik allodiniyi azaltma hem de sinir-kas dokusunda daha fazla fonksiyonel iyileşme sağladığı tespit edildi.

Keywords

Mezenkimal kök hücre, periferik nöropati, ağrı.

Ethical Statement

Ethical approval was obtained from the Local Ethics Committee of Animal Experiments of Cukurova University with the decision dated 29.11.2011 and numbered 17.

Supporting Institution

This study was supported by grants from theCukurova University Scientific Research Projects Unit, project no. TF2011BAP12.

Thanks

We would like to thank Berk Çelikyürek for his editing contributions.

The effects of mesenchymal stem cells applied during the subacute period in peripheral neuropathy

Abstract

Purpose: The study aims to investigate the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) administered subacute period to neuropathic mice on allodynia and nerve-muscle tissue functions during 24 weeks.
Materials and Methods: Peripheral neuropathy was induced by partial sciatic nerve ligation. Experiments were conducted in Control, Sham, Neuropathic, BM-MSC, and Neuropathic+BM-MSC groups. Allodynia was measured by cold plate test at the 2nd, 6th, and 24th weeks. Electrophysiological and histopathological examinations were performed on isolated nerve-muscle tissues at the end of the 24th week.
Results: Allodynia threshold increased in the Neuropathic+BM-MSC group (7.76±0.33 sec) from the 6th week and continued to increase along 24 weeks compared to the Neuropathic group (4.36±0.21 sec). Action potential (137.9±7.85 mV) and depolarization (0.74±0.01 msec) values of the Neuropathic+BM-MSC group exhibited partial improvement compared to the Neuropathic group (121.5±3.03 mV and 0.81±0.02 msec, respectively) at the 24th week. Muscle tissue's resting membrane potential values increased in the Neuropathic+BM-MSC group compared to the Neuropathic group (-73.4±0.2 and -87.7±0.2 mV, respectively). Histopathological examination of nerve tissue revealed loss of myelinated axons and significant fibrosis in the endoneurium in the Neuropathic group while Schwann cell proliferation and preservation of myelinated axons were observed in the Neuropathic+BM-MSC group. Muscle fiber atrophy, compensatory hypertrophic fibers, and increased central nuclei were seen in the Neuropathic group, while small atrophic muscle fiber groups were identified in the Neuropathic+BM-MSC group.
Conclusion: BM-MSC application in the subacute period is found to reduce allodynia and provide functional recovery in nerve-muscle tissue in experimental peripheral neuropathy.

Keywords

mesenchymal stem cell, peripheric neuropathy, pain

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