Farklı Test Sistemleri ile Somatik Hücrelerde Profenofos Genotoksisitesine Karşı Kuşburnu (Rosa canina L.) Ekstrelerinin Doğal Bir Antigenotoksik Ajan Olarak Kullanılması

Year 2016, Volume: 37 Issue: 1, 30 - 40, 06.04.2016

Caner Kasımoğlu Handan Uysal

https://doi.org/10.17776/csj.10890

Abstract

Bu çalışmada, profenofos insektisitinin genotoksik etkileri, Drosophila melanogaster’de somatik mutasyon
ve rekombinasyon testi (SMART) ve insan periferal lenfosit hücrelerinde mikronükleus (MN) testi ile araştırılmıştır.
Bu maddenin olası genotoksik etkilerinin giderilebilmesi için de kuşburnu (Rosa canina) bitkisine ait su ve etanol
ekstreleri kullanılmıştır.
SMART için D.melanogaster’in kanat preparatları incelendiği zaman, profenofosun artan konsantrasyonuna (0,025,
0,05, 0,075 ve 0,1ppm) bağlı olarak normal kanat fenotipinde mutasyon frekansının arttığı özellikle en yüksek
uygulama grubunda (0,1 ppm) bu artışın pozitif etkili (+) olduğu gözlenmiştir (P < 0,05). Serrat kanat fenotipinde ise
mutasyon frekansındaki artış tüm uygulama gruplarında önemsiz etkili (i) bulunmuştur (P > 0,05). Kuşburnu
bitkisinin su (RCsu) ve etanol (RCeta) ekstreleri, profenofosun en yüksek konsantrasyonu (0,1 ppm) ile birlikte
uygulandığı zaman (profenofos+ RCsu/RCeta) mutasyon frekansındaki artışın azaldığı ve bunun da istatistiksel
olarak önemli olduğu gözlenmiştir (P < 0,05).
Bu çalışmada, farklı konsantrasyonlarda in vitro profenofos uygulaması (0,25, 0,5, 0,75 ve 1ppm) ile insan periferal
lenfosit hücrelerinde mikronükleus frekansının arttığı ve nükleer bölünme indeksinin (NBİ) azaldığı da görülmüştür
(P < 0,05). Ancak profenofos+RCsu ve RCeta uygulamaları ile mikronükleus frekansı düşerken NBİ değeri de kontrol
grubuna yaklaşmıştır (P < 0,05).

Keywords

Drosophila melanogaster, Profenofos, SMART, Mikronükleus, Rosa canina

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