Organik-inorganik hibrit nano çiçeklerin çemen (Trigonella foenum-graecum L.) tohum ekstresi kullanılarak sentezi ve anti-mikrobiyal özelliklerinin araştırılması

Year 2019, Volume: 36 Issue: 2, 159 - 167, 10.12.2019

Cevahir Altınkaynak , Nilay Ildız Ayşe Baldemir , Nalan Özdemir , Vedat Yılmaz İsmail Öçsoy

https://doi.org/10.16882/derim.2019.549151

Cited By: 26

Abstract

Bu çalışmada organik-inorganik hibrit nano çiçek
sentezi için organik bileşen olarak çemen (Trigonella
foenum-graecum L.) otu tohum
ekstresi ve inorganik bileşen olarak Cu²⁺ iyonları kullanılarak
yeşil bir üretim metodu raporlanmıştır. Çemen tohum ekstresi kullanılarak
sentezlenmiş organik-inorganik hibrit nano çiçekler (TF-Cu²⁺ hNF); taramalı elektron mikroskobu (SEM), Enerji Dağılımlı Spektrometre (EDX),
X-ışını kırınımı (XRD) ve Kızılötesi spektroskopisi (FT-IR) yöntemleri
kullanılarak karakterize edilmiştir. TF-Cu²⁺ hNF’lerin elektron
mikroskop görüntüsünde morfolojisi oldukça düzgün dağılımlı ve küresel formda
olmak üzere yaklaşık 18 μm boyutunda
gözlenmiştir. TF-Cu²⁺
hNF’ler; Pseudomonas aeruginosa and Haemophilus influenza
dışında Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus,
Bacillus cereus, Salmonella typhi ve Escherichia coli türlerine
karşı 1-10 µg ml^-1 aralığında ve kullanılan antibiyotikler
ile karşılaştırıldığında yüksek düzeyde
anti-mikrobiyal özellik göstermiştir. Ancak hem TF-Cu²⁺ hNF’ler hemde serbest TF ekstresi, Candida
albicans ve Candida glabrata türlerine karşı antifungal aktivite
göstermemiştir. Yapılan bu çalışma
çemen ekstresi içeren organik-inorganik hibrit nano çiçeklerin test edilen
patojen suşlar ile gelişen mikrobiyal enfeksiyonlar için terapötik bir ajan
olarak kullanılabileceğini ve ilaç direncinin üstesinden gelme potansiyeline
sahip olduğunu ortaya koymaktadır.

Keywords

Biyomateryal, Organik - inorganik hibrid nano çiçekler, Bakır, Anti-mikrobiyal aktivite

Synthesis of organic-inorganic hybrid nanoflowers using Trigonella foenum-graecum seed extract and investigation of their anti-microbial activity

Abstract

Herein
we report a green method for the synthesis of organic-inorganic hybrid
nanoflowers using a Trigonella
foenum-graecum L. (TF) (Fenugreek seeds) extracts as an organic part and
copper ions acting as an inorganic part. The organic-inorganic hybrid
nanoflowers using TF seed extract (TF-Cu²⁺ hNFs) were characterized
by SEM, XRD, EDX and FTIR. The morphology of the TF-Cu²⁺ hNFs was
quite spherical and monodisperse with ∼18μm size. The TF-Cu²⁺
hNF exhibited the effective anti-bacterial activity against Enterococcus
faecium, Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus,
Salmonella typhi and Escherichia coli at 1-10 µg ml^-1
concentrations except against Pseudomonas aeruginosa and Haemophilus
influenza. However, both TF-Cu²⁺ hNFs and free TF extracts
showed any antifungal activities against Candida albicans or Candida
glabrata. The study revealed that TF-Cu²⁺ hNFs could be used as
a therapeutic agent for microbial infections and has the potential to overcome
drug resistance.

Keywords

Biomaterials, Organic-inorganic hybrid nanoflowers, Copper, Anti- microbial activity

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