Synthesis Optimization and Inhibition Effects of Bismuth-BAL Chelate on Escherichia coli, Streptococcus pyogenes, and Activated Sludge

Year 2019, Volume: 21 Issue: 62, 499 - 508, 21.05.2019

Börte Köse Mutlu , Türker Türken , Selin Okatan Gamze Durmaz Gülsüm Melike Ürper Bayram Serkan Güçlü Süleyman Övez , İsmail Koyuncu

https://doi.org/10.21205/deufmd.2019216215

Abstract

With the development of technology
and engineering, different highly qualified products can be manufactured and
used with a wide range. This highly qualified micro- and nano-technological
materials can be easily covered with bacteria colonies and biofilms.
Researchers have tried to synthesize different anti-bacterial chemicals to
solve this problem. The formation of biofilm on the surface and in the pores,
which is caused by the extracellular polymeric substances (EPS) and soluble
microbial products (SMP), has long been identified as a problem for engineering
applications. The total polysaccharides and proteins secreted by microorganisms
can be decreased when exposed to the synthesized bismuth-BAL chelate (BisBAL).
Our proposal is to inhibit the EPS and SMP from Escherichia coli and Streptococcus
pyogenes. The anti-bacterial properties of synthesized chemicals that have
several combinations of bismuth metal with lipophilic thiol compound were
examined to get the optimum inhibitor. The application strategies and stability
of BisBAL were also studied. The chelate can be used as a compound of membranes
used in membrane bioreactors, which will be an obligation of advanced
wastewater treatment with the aims of the protection of natural water sources’
quality and supply the water demand, to prevent the biofouling. 

Keywords

Anti-bacterial Product, Bismuth, Minimum Inhibition Concentration, Mixed Culture, Pure Bacteria Culture

Bizmut-BAL Şelatı Sentezinin Optimizasyonu ve Escherichia coli, Streptococcus pyogenes ve Aktif Çamur Üzerindeki İnhibisyon Etkisi

Abstract

Teknoloji
ve mühendisliğin gelişmesi ile beraber yüksek kalitede pek çok ürünün üretimi
ve geniş kullanım alanlarında yeralması mümkün olmaktadır. Bu yüksek kalitedeki
mikro- ve nano-teknolojik malzemeler bakteri kolonileri ve biyofilm tabakaları
tarafından kolayca sarılmaktadırlar. Araştırmacılar, bu probleme çözüm
bulabilmek adına pek çok farklı antibakteriyel kimyasal sentezlemişlerdir. Uzun
bir süredir, hücredışı polimerik maddeler (EPS) ve çözünen mikrobiyal ürünler
(SMP) sebebiyle porlarda ve yüzeylerde gerçekleşen biyofilm oluşumu mühendislik
uygulamalarının bir problemi olarak gösterilmektedir. Mikroorganizmalar
sentezlenen bizmut-BAL şelatı (Bis-BAL) na maruz kaldığında toplam
polisakkaritleri ve proteinleri daha az salgılarlar. Bu çalışmada Escherichia coli ve Streptococcus pyogenes tarafından salgılanacak EPS ve SMP’nin
engellenmesi öngörülmüştür. Bizmut metali ve lipofilik tiyolün çeşitli
konsantrasyonlarının kombinasyonlar halinde denenmesi ile sentezlenen
kimyasallar arasından en yüksek inhibisyon etkisini gönderenin belirlenmesi
amaçlanmıştır. BisBAL’ın uygulanmasıda izlenebilecek stratejiler ve stabilitesi
de çalışılmıştır. İlgili şelat membran biyoreaktörlerde kullanılacak olan
membranların bünyesine ilave edilebilir ve böylelikle doğal su kaynaklarının
kalitesinin korunarak su ihtiyacının karşılanmasında tercih edilen membran
teknolojisinde biyotıkanmanın önüne geçilebilir.

Keywords

Antibakteriyel Ürün, Bizmut, Minimum İnhibisyon Konsantrasyonu, Karışık Kültür; Saf Bakteri Kültürü

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