Determination of Antioxidant Effect of Walnut (Juglans regia L.) on Lung and Muscle Tissue

Year 2019, Volume: 2 Issue: 1, 29 - 39, 30.04.2019

Bedia Bati , İsmail Çelik , Abdullah Turan

Abstract

This study was to
investigate the antioxidant role of Juglans
regia L. (walnut) against ethanol-induced oxidative stress in lung and
muscle tissues. Female Albino
36 rats were divided into six groups and treated as follows: I (control), II
(20% ethanol), III (10%walnut), IV (20% ethanol + 10%walnut), V (5% walnut) and
VI (20% ethanol + 5% walnut). The
rats were sacrificed after 50 days of administration and the tissues
were analyzed after isolation. According to the results, it was found that the
increased malondialdehyde (MDA) content owing to the alcohol-induced oxidative
stress in both tissues decreased in the walnut treated tissues. In addition alcohol
and alcohol + walnut treatment of nutritional supplemented rats changed catalase
(CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST) and superoxide
dismutase (SOD) content and antioxidant defence system (ADS) components
compared to control rats. As a
result, ethanol has been shown to cause fluctuations in ADS components as a
result of oxidative stress in rats and it can be said that walnut has a
curative effect on the this complications caused by oxidative stress.

Keywords

Juglans regia L., Antioxidant defense system, Malondialdehyde

References

• Abdollahi, M., Ranjbar, A., Shadnia, S., Nikfar, S., Rezaiee, A., 2004. Pesticides and oxidative stress: a review. Medical Science Monitor, 10(6), 41-47.
• Aebi, H., 1974, Catalase, ‘‘In Methods of Enzymatic Analysis’’. In H. U. Bergemeyer (Ed.), Academic Press: New York 673-684.
• Aykac, G., Uysal, M., Yalcin, A. S., Koçak-Toker, N., Sivas, A., Öz, H., 1985. The effect of chronic ethanol ingestion on hepatic lipid peroxide, glutathione, glutathione peroxidase and glutathione transferase in rats. Toxicology, 36, 71–76.
• Bagnyukova, T.V., Vasylkiv, O.Y., Storey KB, Lushchak VI., 2005. Catalase inhibition by amino triazole induces oxidative stress in goldfish brain. Brain Research, 1052(2), 180-6.
• Bati, B., Celik, I., Dogan, A., 2015. Determination of hepatoprotective and antioxidant role of walnuts against ethanol-induced oxidative stress in rats. Cell Biochemistry and Biophysics, 71(2), 1191-1198.
• Beutler, E., Dubon, O.B., Kelly, M., 1963. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine, 61, 882–888.
• Bhor, V.M., Raghuram, N., Sivakami, S., 2004. Oxidative damage and altered antioxidant enzyme activities in the small intestine of streptozotocin-induced diabetic rats. The International Journal of Biochemistry & Cell Biology, 36(1), 89-97.
• Carlberg, I., Mannervik, B., 1975. Purification and characterization of the flavoenzyme glutathione reductase from rat live. Journal of Biological Chemistry, 250, 5475–5480.
• Celik, I., Temur, A., Isık, I., 2015. Hepatoprotective role and antioxidant capacity of pomegranate (Punica granatum L.). Cell Biochem Biophys, 71, 1191–1198.
• Christaki. E., Bonos, E., Giannenas, I., Florou-Paneri, P., 2012. Aromatic plants as a source of bioactive compounds. Agriculture, 2, 228-243.
• Chu, Y., Sun, J., Wu, X., Liu, R.H., 2002. Antioxidant and antiproliferative activity of common vegetables. Journal of Agricultural and Food Chemistry 50, 6910-6916.
• Cynamon, H.A., Isenberg, J.N., Nguyen, C.H., 1985. Erythrocyte malondialdehyde release in vitro: a functional measure of vitamin E status. Clinica Chimica Acta, 151(2), 169-76.
• Dogan, A., Celik, I., 2012. Hepatoprotective and antioxidant activities of grape seeds against ethanol-induced oxidative stress in rats. British Journal of Nutrition, 107, 45–51.
• Erukainure, O.L., Onifade, O.F., Odjobo, B.O., Olasehinde, T.A., Adesioye, T.A., Tugbobo-Amisu, A.O., Okonrokwo, G.I., 2017. Ethanol extract of Tetrapleura tetraptera fruit peels: Chemical characterization, and antioxidant potentials against free radicals and lipid peroxidation in hepatic tissues. Journal of Taibah University for Science.
• Erukainure, O.L., Ebuehi, O.A.T., Adeboyejo, F.O., Aliyu, M., Elemo, G.N., 2014. Modulatory effect of fibre-enriched cake on alloxaninduced diabetic toxicity in rat brain tissues. Toxicology Reports, 1, 445–449.
• Fukuda, T., Ito, H., Yoshida, T., 2004. Effect of the walnut polyphenol fraction on oxidative stress in type 2 diabetes mice. BioFactors, 21(1–4), 251–253.
• Gallagher, E.P., Buetler, T.M., Stapleton, P.L., Wang, C., Stahl. D.L., Eaton, D.L., 1995. The effects of diquat and ciprofibrate on mRNA expression and catalytic activities of hepatic xenobiotic metabolizing and antioxidant enzymes in rat liver. Toxicology and Applied Pharmacology, 134(1), 81-91.
• Gutteridge, J.M.C., 1993. Free radicals in disease processes: a compilation of cause and consequence. Free radical research communications, 19, 141-158.
• Halliwell, B., Gutteridge, J.M., 1995. The definition and measurement of antioxidants in biological systems. Free Radical Biology and Medicine, 18(1), 125-126.
• Halliwell. B., Gutteridge, J.M.C., Cross, C.E., 1992. Free radicals, antioxidants, and human disease: where are we now? Journal of Laboratory and Clinical Medicine, 119, 598-620.
• Halvorsen, B.L., Holte, K., Myhrstad, M.C., Barikmo, I., Hvattum, E., Remberg, S.F., Wold, A.B., Haffner, K., Baugerød, H., Andersen, L.F., Moskaug, Ø., Jacobs, D.R. Jr., Blomhoff, R., 2002. A Systematic screening of total antioxidants in dietary plants. Journal of Nutrition, 132, 461–471.
• Jain, S.K., McVie, R., Duett, J., Herbst J.J., 1989. Erythrocyte membrane lipid peroxidation and glycolylated hemoglobin in diabetes. Diabetes, 38, 1539–1543.
• Jiang, W.D., Hu, K., Zhang, J.X., Liu, Y., Jiang. J., Wu, P., Zhao, J., Kuang, S.Y., Tang, L., Tang, W.N., Zhang, Y.A., Zhou, X.Q., Feng, L., 2015. Soyabean glycinin depresses intestinal growth and function in juvenile Jian carp (Cyprinus carpio var Jian): protective effects of glutamine. British Journal of Nutrition, 114(10), 1569-1583.
• Lopez-Alarc ona, C., Denicola, A., 2013. Evaluating the antioxidant capacity of natural products: a review on chemical and cellular-based assays. Analytica Chimica Acta, 763, 1-10.
• Mannervik, B., Guthenberg, C., 1981. Glutathione S-transferase (Human Plasenta). Methods in Enzymology 77, 231–235.
• Maulik, N., McFadden, D., Otani, H., 2013. Thirunavukkarasu M, Parinandi NL. Antioxidants in longevity and medicine. Oxidative Medicine and Cellular Longevity, 2013, 1-3. Article ID 820679, http://dx.doi.org/10.1155/2013/820679.
• McCord, J.M., Fridovich, I., 1969. Superoxide dismutase, An enzymatic function for erythrocuprein (hemocuprein). Journal of Biological Chemistry, 244, 6049–6053.
• Meister, A., Anderson, M.E., 1983. Glutathione. Annual Review of Biochemistry, 52(1), 711-60.
• Mosoni, L., Balage, M., Vazeille, E., Combaret, L., Morand, C., Zagol-Ikapitte, I., Dardevet, D., 2010 Antioxidant supplementation had positive effects in old rat muscle, but through better oxidative status in other organs. Nutrition, 26(11), 1157-1162.
• Oboh, G., Rocha, J.B.T., 2007. Antioxidant in Foods: A New Challenge for Food Processors: Leading Edge Antioxidant Research, Nova Science Publishers Inc., New York, US, 35-64.
• Paglia, D.E., Valentine, W.N., 1967. Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. Journal of Laboratory and Clinical Medicine, 70, 158-169.
• Persson, T., Popescu, B.O., Cedazo-Minguez, A., 2014 Oxidative stress in Alzheimer's disease: why did antioxidant therapy fail. Oxidative Medicine and Cellular
• Longevity, 2014, 1-11 Article ID 427318, http://dx.doi.org/10.1155/2014/427318.
• Pisoschi, A.M., Pop, A., 2015. The role of antioxidants in the chemistry of oxidative stress: A review. European journal of medicinal chemistry, 97, 55-74.
• Poljsak, B., Suput, D., Milisav, I., 2013. Achieving the balance between ROS and antioxidants: when to use the synthetic antioxidants. Oxidative Medicine and Cellular
• Longevity 2013, 1-11. Article ID 956792, http://dx.doi.org/10.1155/2013/ 956792.
• Poulose, S.M., Miller, M.G., Shukitt-Hale, B., 2014. Role of Walnuts in Maintaining Brain Health with Age. Journal of Nutrition, 144, 561-6.
• Salcedo, C.L., de Mishima, B.A.L., Nazareno, M.A., 2010. Walnuts and almonds as model systems of foods constituted by oxidisable, pro-oxidant and antioxidant factors. Food research international, 43(4), 1187-1197.
• Sies, H., 1985. Oxidative Stress: Introductory Remarks. Academic Press, London, 1985.
• Sies, H., 1991. Oxidative stress: from basic research to clinical application, The American Journal of Medicine. 91, 31-38.
• Smith, G.J., Litwack, G., 1980. Roles of ligandin and the glutathione S-transferases in binding steroid metabolites, carcinogens and other compounds. Reviews in Biochemical Toxicology, 2, 1–47.
• Sonde, V., D’souza, A., Tarapore, R., Pereira, L., Khare, M.P., Sinkar, P., Krishnan, S., Rao, C.V., 2000. Simultaneous administration of diethylphthalate and ethyl alcohol and its toxicity in male Sprague-Dawlwy rats. Toxicology, 147, 23–31.
• Thangthaeng, N., Poulose, S.M., Fisher, D.R., Shukitt-Hale, B., 2018. Walnut extract modulates activation of microglia through alteration in intracellular calcium concentration. Nutrition Research 49. 88-95
• Toda, S., Miyase, T., Arichi, H., Takino, Y., 1985. Natural anti- oxidants III. Antioxidative components isolated from rhizome of Curcuma longa L. Chemical and Pharmaceutical Bulletin, 33 (4), 1725-1728.
• Toda, S., 2011. Polyphenol content and antioxidant effects in herb teas. Chinese Medicine, 2, 29-31.
• Torabian, S., Haddad, E., Rajaram, S., Banta, J., Sabate, J., 2009. Acute effect of nut consumption on plasma total polyphenols, antioxidant capacity and lipid peroxidation. Journal of Human Nutrition and Dietetics, 22, 64-71.
• Turan, A., Çelik, İ., 2016. Antioxidant and hepatoprotective properties of dried fig against oxidative stress and hepatotoxicity in rats. International Journal of Biological Macromolecules, 91, 554–559.
• Van Der Vliet, A., 2015. Antioxidant Defenses in the Lung. Comparative Biology of the Normal Lung, 25, 489-507.
• Venkatachalam, M., Sathe, S.K., 2006. Chemical composition of selected edible nut seeds. Journal of Agricultural and Food Chemistry, 54, 4705–4714.
• Willis, L.M., Bielinski, D.Fç, Fisher, D.R., Matthan, N.R., Joseph, J.A., 2010.Walnut extract inhibits LPSinduced activation of BV-2 microglia via internalization of TLR4: possible involvement of phospholipase D2. Inflammation, 33, 325-33.
• Wu, X., Cao, W., Jia, G., Zhao, H., Chen, X., Wu, C., Liu, G., 2017. New insights into the role of spermine in enhancing the antioxidant capacity of rat spleen and liver under oxidative stress. Animal Nutrition, 3(1), 85-90.
• Xi, M., Hai, C., Tang, H., Chen, M., Fang, K., Liang, X., 2008. Antioxidant and antiglycation properties oftotalsaponins extracted fromtraditional chinese medicine used to treat diabetes mellitus. Phytotherapy Research, 22, 228–237.
• Xu, D.P., Li, Y., Meng, X., Zhou, T., Zhou, Y., Zheng, J., Zhang, J.J., Li, H.B., 2017. Natural antioxidants in foods and medicinal plants: extraction, assessment and resources. International Journal of Molecular Sciences, 18(1), 96.
• Yurt, B., Celik, I., 2011. Hepatoprotective effect and antioxidant role of sun, sulphited-dried apricot (Prunus armeniaca L.) and its kernel against ethanol-induced oxidative stress in rats. Food and Chemical Toxicology, 49(2), 508–513.