Mikrodalga Enerjisinin Buğdayda Çimlenme, Erken Fide Büyümesi ve Mitotik Bölünme Üzerine Etkisi

Year 2019, Volume: 2 Issue: 2, 61 - 68, 30.04.2019

Ayten Eroğlu , Nlüfer Çiriğ Selçuk

Abstract

Bu çalışmada, mikrodalga enerjisinin buğday  (Triticum
aestivum L. var Tir  ve Bezostaja) tohumlarında
çimlenme,  erken fide büyümesi ve  mitotoik bölünme üzerindeki etkisinin araştırılması
amaçlanmıştır. İki kışlık buğday çeşidine ait tohumlar mikrodalga fırında  30, 60 ve 90 saniye süre ile orta dereceli
enerjiye  (460 W) maruz bırakılmıştır.
Kontrol olarak mikrodalga uygulanmamış tohumlar kullanılmıştır. Kontrol ve
mikrodalga uygulanmış tohumlar ortalama 25 ^oC sıcaklık ve 16 saat
aydınlık/8 saat karanlık fotoperiyotta 1 hafta süreyle çimlenme ve fide büyümesi
açısından gözlemlenmiştir. 2 günlük çimlenmiş tohumların kök uçları fikse
edildikten  ve asetik orsein ile
boyandıktan sonra mitotik bölünme fazları incelenmiştir. Mikrodalgaya maruz
bırakılmış tohumların çimlenme yüzdeleri kontrol bitkilerine göre önemli oranda
düşük bulunmuştur. Bu etki, sürenin 
uzamasına bağlı olarak artmaktadır. Fidelerin  kök ve gövde uzunluğu ile kök ve gövde yaş
ağırlıkları üzerinde mikrodalga enerjisinin negatif bir etkisi görülmediği gibi
mitotik bölünme fazlarında ve kromozom yapısında  herhangi bir anormallik gözlenmemiştir.

Keywords

: Buğday, mikrodalga enerjisi, çimlenme, mitotik bölünme

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