The relationship between carbonic anhydrase-III expression and oxidative stress in brown adipose tissue

Year 2018, Volume: 45 Issue: 4, 361 - 368, 13.12.2018

Cemil Kahraman , Ahmet Alver Ayşe Şentürk , İmran İnce Akça

https://doi.org/10.5798/dicletip.497874

Cited By: 2

Abstract

Objective:
High-fat foods increase adipose tissue size, and induce obesity. Although
carbonic anhydrase III is abundantly found in brown adipose tissue, its
function is not fully defined. In this study, we investigated the relationship
between carbonic anhydrase III enzyme mRNA expression and malondialdehyde,
oxidative stress marker, in brown adipose tissue of rats that were fed high-fat
diets. In addition, we investigated potential effect of N-acetylcysteine as an
antioxidant in this relationship.

Methods: In
our study three experimental groups were formed and each contained 6 rats
(control, obese, and antioxidant groups). The experimental groups were fed for
a duration of 85 days with high fat diets. In these groups, carbonic anhydrase
III mRNA expression, total carbonic anhydrase hydratase activitie, and
malondialdehyde levels were measured in brown adipose tissues dissected from
rat scapula regions.

Results:
According to our findings, carbonic anhydrase III mRNA expression was higher in
the obese group than in the control group (p = 0.004), and malondialdehyde
levels were lower in the obese group than in the control group (p = 0.03). It
was observed that carbonic anhydrase III mRNA expression was higher in the
antioxidant group than in the control group (p = 0.006), and malondialdehyde
levels were lower in the antioxidant group than in the control group (p =
0.006). In addition, in the obese group carbonic anhydrase III mRNA expression
was higher than in the antioxidant group (p=0.01).

Conclusion:
In brown adipose tissue of rats that were fed high-fat diets, this study showed
that the carbonic anhydrase III mRNA expression increased and the
malondialdehyde level decreased.

Keywords

Carbonic anhydrase III, brown adipose tissue, obesity, N-acetylcysteine

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