IN VITRO PROPAGATION OF ENDEMIC, ENDANGERED PLANT SPECIES AUBRIETA OLYMPICA BOISS.

Year 2016, Issue: 037, 1 - 8, 30.12.2017

Betül Akın , İsmail Kocaçalışkan , Gürcan Güleryüz

Abstract

Turkey
has a rich biological diversity due to its geographical feature. Uludağ
mountain is one of the Important Plant Areas of Turkey. Thus, native plant
species are threatened due to various environmental factors and human damage.
The micropropagation possibility for Aubrieta
olympica Uludağ endemic threatened plant species, were investigated in
this study. The effect of different concentrations of agar and different levels
of pH were evaluated on shoot development. During the proliferation stage, the
best development occured at 0.6% agar and pH 5.7-5.8. Shoot tips and internode
explants were the best source for the highest shoot induction of A.olympica species. The best results for
shoot formation were obtained with 0.5 ppm Kinetin and plant growth
regulators free Murashige and Skoog medium. Elongated shoots were
rooted in the Murashige and Skoog medium supplemented with different concentrations
of indole-3-butyric acid, indole-3-acetic acid, and also a medium without plant
growth regulators.  However,  A.olympica shoots spontaneously rooted on plant growth
regulators free medium. Shoots which developed roots were transferred to
greenhouse, successfully. The results of this study could be utilized for
conservation purposes of endemic and endangered plant species. 

Keywords

Micropropagation, Uludağ, Plant growth regulators

ENDEMİK, NESLİ TEHLİKE ALTINDA OLAN AUBRIETA OLYMPICA BOISS. BİTKİ TÜRÜNÜN IN VITRO ORTAMDA ÇOĞALTILMASI

Abstract

Türkiye
coğrafik özelliklerinden dolayı zengin bir biyolojik çeşitliliğe sahiptir.
Çalışma alanımız olan Uludağ, Türkiye’nin Önemli Bitki Alanlarından birisidir. Bununla
birlikte, doğal bitki türlerimizin nesilleri, çeşitli çevresel ve insan
faktörlerinden dolayı tehlike altındadır. Çalışmamızda, Uludağ endemiği olan ve
nesli tehlike altında olan Aubrieta
olympica türünün doku kültürü şartlarında çoğaltılma imkanı
araştırılmıştır. Farklı agar konsantrasyonları ve farklı pH değerlerinin sürgün
gelişimi üzerine etkileri değerlendirilmiştir. Çoğaltma aşaması süresince en
iyi sürgün gelişimi % 0,6 agar ve pH 5,7-5,8’de meydana gelmiştir. A.
olympica bitkisinde sürgün rejenerasyonu için en iyi eksplant
kaynağının sürgün ucu ve internod olduğu tespit edilmiştir. En iyi sürgün
rejenerasyonu 0,5 ppm Kinetin ve büyüme düzenleyici içermeyen içeren Murashige
and Skoog besin ortamlarında elde edilmiştir. Yeterli uzunluğa ulaşmış
sürgünler farklı konsantrasyonlarda indol-3-bütirik asit, indole-3-asetik asit
ve büyüme düzenleyici içermeyen besin ortamında köklendirilmiştir. Bunun
yanında, A.olympica sürgünleri
bitki büyüme düzenleyici içermeyen besin ortamında kendiliğinden
köklenmiştir. Köklenen sürgünlerin sera şartlarına başarılı bir şekilde
adaptasyonu sağlanmıştır. Bu çalışma sonuçları, diğer endemik ve nesli
tükenmekte olan bitkilerde yapılacak olan koruma çalışmalarına temel
oluşturacaktır. 

Keywords

Çoğaltma, Uludağ, Bitki büyüme düzenleyiciler

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