Deneysel Nörolojik Hastalık Modellemelerinde Anestezi Uygulamaları

Year 2024, Volume: 26 Issue: S1, 30 - 35, 30.06.2024

Ayhan Çetinkaya , Hümeyra Çelik

https://doi.org/10.18678/dtfd.1504037

Abstract

Beynin hangi moleküler mekanizmalar kullanarak nörolojik hastalıkların oluştuğu ve nasıl tedavi edilebileceği kapsamındaki sinir bilim, multidisipliner yaklaşımlar ile erken tanı ve yeni tedavi geliştirmek üzere yüksek bütçeli araştırmaların konusu olarak güncelliğini korumaktadır. Bu amaçla nörolojik in vivo deneysel modellerde uygun modeli doğru metot ve anestezi ile oluşturmak doğru sonuçları elde etmenin ve hayvan refahını sağlamanın en önemli basamaklarıdır. Kemirgenlerde anesteziyi yönetmek adına anesteziklerin fizyolojik özelliklerini tanımak ve risklerine hakim olmak deneysel prosedürleri güçlendirecektir. Enjekte edilebilir anesteziklerden ketamin, ksilazin ve pentobarbital genel anestezi için kısa cerrahi prosedürlerde en sık tercih edilen ajanlardır. İzofluran ve sevofluran sıvı olduklarından bir vaporizer ile verilen inhaler anesteziklerdendir. İnhale anesteziklerin hızlı indüksiyon ve hızlı çekilme gibi önemli avantajları sinirbilim çalışmalarında inhaler anestezikleri öne çıkarmaktadır. Bu derlemede kemirgenlerde sık kullanılan anestezik ajanların özellikleri, kullanım şekilleri ve hangi modelde tercih edildiğinden bahsedilecektir. Bu amaçla epilepsi, Alzeimer hastalığı, iskemi-reperfüzyon hasarı, travmatik beyin hasarı, iskemik inme, deneysel otoimmun ensefalomyelitis, oftalmik cerrahi prosedürler ve yan etkileri gibi hayvan modellerinde uygun anesteziklerin seçimi gözden geçirilecektir.

Keywords

Anestezi, sinirbilim, in vivo modeller

Anesthesia Applications In Experimental Neurological Disease Modeling

Abstract

Neuroscience, which covers the molecular mechanisms employed by the brain to cause neurological diseases and how they can be treated, remains current as the subject of high-budget investigations to develop early diagnosis and new treatment modalities with multidisciplinary approaches. For this purpose, creating the appropriate model with the correct modality and anesthesia in neurological in vivo experimental models is the most important phase to obtain accurate results and ensure animal welfare. To manage anesthesia in rodents, knowing the physiological characteristics of anesthetics and their risks will strengthen experimental procedures. Among the injectable anesthetics, ketamine, xylazine, and pentobarbital are the most frequently preferred agents for general anesthesia in short surgical procedures. Isoflurane and sevoflurane are inhaler anesthetics that are administered through a vaporizer because they are liquids. Important advantages of inhaled anesthetics, such as rapid induction and rapid withdrawal, make inhaled anesthetics stand out in neuroscience studies. In this review, the properties of frequently used anesthetic agents in rodents, their usage methods, and which model they are preferred will be discussed. For this purpose, the selection of appropriate anesthetics in animal models such as epilepsy, Alzheimer's disease, ischemia-reperfusion injury, traumatic brain injury, ischemic stroke, experimental autoimmune encephalomyelitis, and ophthalmic surgical procedures and their side effects will be reviewed.

Keywords

Anesthesia, neuroscience, in vivo models

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