In Vitro Antimicrobial Activity Screening of Leucoagaricus leucothites and Determination of the Ethanol Extract Composition By Gas Chromatography/Mass Spectrometry

Year 2020, Volume: 8 Issue: 2, 1250 - 1257, 30.04.2020

Kerem Canlı , İlgaz Akata , Ali Yetgin , Atakan Benek Ergin Murat Altuner

https://doi.org/10.29130/dubited.642707

Cited By: 1

Abstract

Macrofungi are good food resources, which have not only medicinal properties but are nutritive as well. For centuries they have been used for preventing several diseases including cancer, hypercholesterolemia and hypertension. They are also known to present antimicrobial activity, thus the aim of the present study is to put forward antimicrobial activity of ethanol extract of Leucoagaricus leucothites (Vittad.) Wasser 1977, a macro fungi and in addition to determine the chemical composition by Gas Chromatography-Mass Spectrometry. Nineteen bacteria strains and one yeast strain are used in antimicrobial screening. According to the data obtained from the study L. leucothites presented both antibacterial and antifungal activity against the bacteria and yeast strains used at different concentrations with different levels. Although there are some previous studies, it can be proposed that this study is the first detailed screening report regarding the antibacterial and antifungal potential of L. leucothites and the compounds found in L. leucothites ethanol extract.

Keywords

Leucoagaricus leucothites, Mushroom, Antimicrobial activity, Chemical composition, Disk diffusion method, GC-MS, GC-MS

Leucoagaricus leucothites’in In Vitro Antimikrobiyal Aktivite Taraması ve Etanol Ekstrakt Kompozisyonunun Gaz Kromatografisi/Kütle Spektrometresi ile Belirlenmesi

Abstract

Makro mantarlar sadece tıbbi özelliklere sahip olmayan, aynı zamanda da besleyici olan iyi besin kaynaklarıdır. Yüzyıllar boyunca kanser, hiperkolesterolemi ve hipertansiyon gibi çeşitli hastalıkları önlemek için kullanılmışlardır. Ayrıca makro mantarların antimikrobiyal aktivite gösterdikleri de bilinmektedir, bu nedenle bu çalışmanın ana amacı, bir makro mantar olan Leucoagaricus leucothites (Vittad.) Wasser 1977’nin etanol ekstraktının antimikrobiyal aktivitesini ortaya koymak ve ayrıca Gaz Kromatografisi-Kütle Spektrometresi ile kimyasal bileşimin belirlenmesini sağlamaktır. Antimikrobiyal taramada on dokuz bakteri, bir adet maya suşu kullanılmıştır. Bu çalışmadan elde edilen verilere göre L. leucothites farklı konsantrasyonlarda kullanılan tüm mikroorganizmalara karşı farklı seviyelerde hem antibakteriyel hem de antifungal aktivite ortaya koymuştur. Her ne kadar daha önce yapılmış birkaç çalışma bulunsa da; bu çalışmanın, L. leucothites'in antibakteriyel ve antifungal potansiyeli ve L. leucothites etanol ekstraktında bulunan bileşikler ile ilgili ilk ayrıntılı tarama raporu olduğu öne sürülebilir.

Keywords

Leucoagaricus leucothites, Mantar, Antimikrobiyal aktivite, Kimyasal bileşim, Disk difüzyon yöntemi, GC-MS, GC-MS

References

• [1] F. Watanabe, Y. Yabuta, T. Bito, and F. Teng, “Vitamin B12-Containing plant food sources for vegetarians,” Nutrients, vol. 6, no. 5, pp. 1861-1873, 2014.
• [2] G. Cardwell, J. Bornman, A. James, and L. Black, “A review of mushrooms as a potential source of dietary vitamin D,” Nutrients, vol. 10, no. 10, pp. 1498, 2018.
• [3] J. Falandysz, “Selenium in Edible Mushrooms,” J. Environ. Sci. Heal. C, vol. 26, no. 3, pp. 256-299, 2008.
• [4] M. Bonatti, P. Karnopp, H. M. Soares, and S. A. Furlan, “Evaluation of Pleurotus ostreatus and Pleurotus sajor-caju nutritional characteristics when cultivated in different lignocellulosic wastes,” Food Chem., vol. 88, no. 3, pp. 425-428, 2004.
• [5] D. Agrahar-Murugkar and G. Subbulakshmi, “Nutritional value of edible wild mushrooms collected from the Khasi Hills of Meghalaya,” Food Chem., vol. 89, no. 4, pp. 599-603, 2005.
• [6] L. M. Cheung and P. C. Cheung, “Mushroom extracts with antioxidant activity against lipid peroxidation,” Food Chem., vol. 89, no. 3, pp. 403-409, 2005.
• [7] A. Imtiaj and T. S. Lee, “Screening of antibacterial and antifungal activities from Korean wild mushrooms,” World J Agric. Sci., vol. 3, no. 3, pp. 316-321, 2007.
• [8] J. Clardy and C. Walsh, “Lessons from natural molecules,” Nature, vol. 432, no. 7019, pp. 829-837, 2004.
• [9] D. Webster, P. Taschereau, R.J. Belland, C. Sand, and R. P. Rennie, “Antifungal activity of medicinal plant extracts; preliminary screening studies,” J. Ethnopharmacol., vol. 115, no. 1, pp. 140-146, 2008.
• [10] K. Canli, E. M. Altuner, I. Akata, Y. Turkmen, and U. Uzek, “In vitro antimicrobial Screening of Lycoperdon lividum and determination of the ethanol extract composition by gas chromatography/mass spectrometry,” Bangladesh J. Pharmacol., vol. 11, no. 2, pp. 389-394, 2016.
• [11] K. Canli, I. Akata, and E. M. Altuner, “In vitro antimicrobial activity screening of Xylaria hypoxylon,” Afr. J. Tradit. Complement. Altern. Med., vol. 13, no. 4, pp. 42-46, 2016.
• [12] WHO. (2019, Aug 15). Antibiotic resistance [Online]. Available: https://www.who.int/news-room/fact-sheets/detail/antibiotic-resistance.
• [13] B. Paudel, H. D. Bhattarai, J. S. Lee, S. G. Hong, H. W. Shin, and J. H. Yim, “Antibacterial potential of antarctic lichens against human pathogenic gram‐positive bacteria,” Phytother. Res., vol. 22, no. 9, pp. 1269-1271, 2008.
• [14] E. M. Altuner, K. Canli, and I. Akata, “Antimicrobial screening of Calliergonella cuspidata, Dicranum polysetum and Hypnum cupressiforme,” J. Pure Appl. Microbio., vol. 8, no. 1, pp. 539-545, 2014.
• [15] N. Bala, E. A. Aitken, N. Fechner, A. Cusack, and K. J. Steadman, “Evaluation of antibacterial activity of Australian basidiomycetous macrofungi using a high-throughput 96-well Plate assay,” Pharm. Biol., vol. 49, no. 5, pp. 492-500, 2011.
• [16] B. Dülger, F. Şen, and F. Gücin, “Antimicrobial activity of the macrofungi Russula delica Fr.,” Turkish J. Biol., vol. 23, no. 1, pp. 127-134, 1999.
• [17] N. Gunde-Cimerman, “Medicinal value of the genus Pleurotus (Fr.) P. Karst.(Agaricales Sl, Basidiomycetes),” Int. J. Med. Mushrooms, vol. 1, no. 1, pp. 69-80, 1999.
• [18] V. E. C. Ooi, “Medicinally important Fungi,” in Science and Cultivation of Edible Fungi, V. Griensven, Ed., Rotterdam: Balkema, 2010, pp. 41-51.
• [19] S. P. Wasser and A. L. Weis, “Medicinal properties of substances occurring in higher basidiomycetes mushrooms: current perspectives,” Int. J. Med. Mushrooms, vol. 1, no. 1, pp. 31-62, 1999.
• [20] S. P. Wasser and A. L. Weis, “Therapeutic effects of substances occurring in higher basidiomycetes mushrooms: a modern perspective,” Crit. Rev. Immunol., vol. 19, no. 1, pp. 65-96, 1999.
• [21] B. Dulger, T. B. Suerdem, D. Yesilyurt, N. Hacioglu, and A. Camdeviren, “Evaluation of antimicrobial activity of the macrofungus Phellinus torulosus,” J. Biol. Sci., vol. 5, no. 4, pp. 436-439, 2005.
• [22] E. M. Altuner and K. Canli, “In vitro antimicrobial screening of Hypnum andoi AJE Sm,” Kastamonu Üniv. Orman Fak. Derg., vol. 12, no. 1, pp. 97-101, 2012.
• [23] E. M. Altuner, “Bazı karayosunu türlerinin antimikrobiyal aktivitesinin belirlenmesi”, Doktora tezi, Ankara Üniversitesi, Ankara, Türkiye, 2008.
• [24] K. A. Hammer, C. F. Carson, and T. V. Riley, “Antimicrobial activity of essential oils and other plant extracts,” J. Appl. Microbiol., vol. 86, no. 6, pp. 985-990, 1999.
• [25] E. M. Altuner, I. Akata, and K. Canli, “In vitro antimicrobial activity screening of Bovista nigrescens Pers,” Kastamonu Üniv. Orman Fak. Derg., vol. 12, no. 1, pp. 90-96, 2012.
• [26] K. Canli, A. Yetgin, I. Akata, and E. M. Altuner, “In vitro antimicrobial screening of Aquilaria agallocha roots,” Afr. J. Tradit. Complement. Altern. Med., vol. 13, no. 5, pp. 178-181, 2016.
• [27] J. M. Andrews, “BSAC standardized sisc susceptibility testing method (Version 6),” J. Antimicrob. Chemother., vol. 60, no. 1, pp. 20-41, 2007.
• [28] A. M. Mahasneh and A. A. El-Oqlah, “Antimicrobial activity of extracts of herbal plants used in the traditional medicine of Jordan,” J. Ethnopharmacol., vol. 64, no. 3, pp. 271-276, 1999.
• [29] S. Silici and A. N. Koc, “Comparative study of in vitro methods to analyse the antifungal activity of propolis against yeasts isolated from patients with superficial mycoses,” Lett. Appl. Microbiol., vol. 43, no. 3, pp. 318-324, 2006.
• [30] M. A.Hossain and A. Rahman, “Chemical composition of bioactive compounds by gc-ms screening and anti-fungal aroperties of the crude extracts of cabbage samples,” Asian J. Biotechnol., vol. 3, pp. 68-76, 2011.
• [31] K. Canli, A. Yetgin, I. Akata, and E. M. Altuner, “Antimicrobial activity and chemical composition screening of Anacyclus pyrethrum Root,” Indian J. Pharm. Educ., vol. 51, pp. 244-248, 2017.
• [32] K. Canli, A. Yetgin, I. Akata, and E. M. Altuner, “Antimicrobial activity and chemical composition screening of Epilobium montanum Root,” Indian J. Pharm. Educ., vol. 51, pp. 239-243, 2017.
• [33] Core R Team. (2019, Aug 15). R: A language and environment for statistical computing [Online]. Available: https://www.R-project.org/.
• [34] C. S. Kalaivani, S. S. Sathish, N. Janakiraman, and M. Johnson, “Gc-ms studies on Andrographis paniculata (Burm. f.) Wall. Ex Nees-a medicinally important plant,” Indian Int. J. Med. Arom. Plants, vol. 2, no.1, pp. 69-74, 2012.
• [35] M. M. Rahman, S. H. Ahmad, M. T. M. Mohamed, and M. Z. Ab Rahman, “Antimicrobial compounds from leaf extracts of Jatropha curcas, Psidium guajava, and Andrographis paniculata,” Sci. World J., 2014, Art no. 635240.
• [36] C. B. Huang, B. George, and J. L. Ebersole, “Antimicrobial activity of n-6, n-7 and n-9 Fatty acids and their esters for oral microorganisms,” Arch. Oral. Biol., vol. 55, no. 8, pp. 555-560, 2010.
• [37] C. S. Lunde, S. R. Hartouni, J. W. Janc, M. Mammen, P. P. Humphrey, and B. M. Benton, “Telavancin disrupts the dunctional integrity of the bacterial membrane through targeted interaction with the cell wall precursor lipid II,” Antimicrob. Agents Chemother., vol. 53, no. 8, pp. 3375-3383, 2009.
• [38] B. Aslim and S. Ozturk, “Phenolic composition and antimicrobial and antioxidant activities of Leucoagaricus leucothites (Vittad.) Wasser,” J. Med. Food, vol. 14, no. 11, pp. 1419-1424, 2011.
• [39] M. Sevindik, A. Rasul, G. Hussain, H. Anwar, M. K. Zahoor, I. Sarfraz, K S. Kamran, H. Akgul, I. Akata, and Z. Selamoglu, “Determination of anti-oxidative, anti-microbial activity and heavy metal contents of Leucoagaricus leucothites,” Pak. J. Pharm. Sci., vol. 31, no. 5, pp. 2163-2168, 2018.