Determination Of In Vitro Antimicrobial Activities Of Some Moss

Year 2021, Volume: 9 Issue: 3 - Additional Issue, 474 - 481, 29.05.2021

Atakan Benek , Merve Şenturan , Özcan Şimşek , Kerem Canlı , Ergin Murat Altuner

https://doi.org/10.29130/dubited.872689

Abstract

Mosses have been known for many years to be effective against diseases and they are successfully used in areas such as treating wounds and fighting infections. In this study, ethyl alcohol extracts of Plagiomnium elatum (Bruch & Schimp.) T.J.Kop., Rhynchostegium megapolitanum (Blandow. ex F. Weber & D. Mohr) Schimp, Tortella bambergeri (Schimp.) Broth and Tortella tortuosa (Hedw.) Limpr were tested using disk diffusion method on a total of 18 microorganisms, 8 gram positive, 9 gram negative bacteria and 1 yeast, and their antimicrobial activities were determined. As a result of the disk diffusion test, it was demonstrated that all moss samples had antimicrobial activity against several strains, but they had significant antimicrobial activities especially on gram positive Staphylococcus aureus ATCC 25923 and gram negative Escherichia coli ATCC 25922.

Keywords

Plagiomnium elatum, Rhynchostegium megapolitanum, Tortella bambergeri, Tortella tortuosa, Antimicrobial activity, Disk diffusion

Bazı Karayosunu Türlerinin İn Vitro Antimikrobiyal Aktivitelerinin Belirlenmesi

Abstract

Karayosunlarının hastalıklara karşı etkisi olduğu çok uzun yıllardan beri bilinmektedir ve yaraların tedavi edilmesi ve enfeksiyonlar ile mücadele gibi alanlarda etkin olarak kullanılmaktadırlar. Bu çalışmada Plagiomnium elatum, Rhynchostegium megapolitanum, Tortella fasciculata ve Tortella tortuosa karayosunu örneklerinin etil alkol ekstraktları disk difüzyon yöntemi kullanılarak, 8 gram pozitif, 9 gram negatif bakteri ve 1 maya olmak üzere, toplam 18 mikroorganizma üzerinde test edilerek antimikrobiyal aktiviteleri belirlenmiştir. Disk difüzyon testi sonucunda tüm karayosunu örneklerinin birkaç mikroorganizmaya karşı antimikrobiyal aktiviteye sahip olduğu ve özellikle gram pozitif olan Staphylococcus aureus ATCC 25923 ve gram negatif olan Escherichia coli ATCC 25922 üzerinde belirgin bir antimikrobiyal aktivitesinin olduğu ortaya konmuştur.

Keywords

Plagiomnium elatum,, Rhynchostegium megapolitanum, Tortella bambergeri, Tortella tortuosa, Antimikrobiyal aktivite, Disk difüzyon

References

• [1] B. Dülger, A. Gönüz, “Antimicrobial Activity of Certain Plants Used in Turkhish Traditional Medicine,” Asian Journal of Plant Sciences, vol.3, no.4, pp.104-107, 2004.
• [2] A. E. Erdoğan, A. Everest, “Antimikrobiyal Ajan Olarak Bitki Bileşenleri,” Türk Bilimsel Derlemeler Dergisi, c.6, s.2, ss.27-32, 2013.
• [3] E. Faydaoğlu, M. Sürücüoğlu, “Tıbbi ve aromatik bitkilerin antimikrobiyal, antioksidanaktiviteleri ve kullanım olanakları,” Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, c.6, s.2, ss.233-265, 2013.
• [4] S. Ursavaş, R. Söyler, “Quantities and sales data of harvested mosses base on Regional Directorate of Forestry,” Kastamonu Üniversitesi Orman Fakültesi Dergisi, vol.15, no.2, pp. 241-251, 2015.
• [5] E. M. Altuner, B. Çetin, “Antimicrobial activity of Isothecium alopecuroides and potential effect of some climate elements on the activity of this bryophyte sample,” Kastamonu Üniversitesi Orman Fakültesi Dergisi, vol.18, no.2, pp.126-137, 2018.
• [6] S. Ursavaş, (2009) “Karayosunları ve ciğerotlarında tür ve çeşitlilik,” Ankara Üniversitesi Biyoloji Anabilim Dalı, seminer kitabı, ss. 1-45.
• [7] S. Ursavaş, Z. Işın, ‘’New records of Bryum gemmiferum and Atrichum crispum for Turkey,’’ Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, vol. 5, no.153, pp.686-690, 2019.
• [8] A. Erdağ, H. Kürschner, “Türkiye bitkileri listesi, Karayosunları: eklentiler 2016-2017,” Bağbahçe Bilim Dergisi, c.5, s.2, ss.10-16, 2018.
• [9] E. M. Altuner, B. Çetin, B. Çökmüş, “Tortella tortulosa (Hedw.) Limpr. Özütlerinin Antimikrobiyal Aktivitesi,” Kastamonu Üniversitesi Orman Fakültesi Dergisi, c.10, s.2, ss.111-116, 2010.
• [10] K. Canlı, A. Yetgin, I. Akata, E. M. Altuner, “Antimicrobial activity and chemical composition screening of Epilobium montanum root,” Indian Journal of Pharmaceutical Education and Research, vol. 51, no. 3, pp. 239-243, 2017.
• [11] K. Canlı, E. M. Altuner, I. Akata, “Antimicrobial screening of Mnium stellare,” Bangladesh Journal of Pharmacology, vol.10, pp. 321-325, 2015.
• [12] E. M. Altuner, K. Canlı, I. Akata, “In vitro Antimicrobial Screening of Hedwigia ciliata var. leucophaea and Determination of the Ethanol Extract Composition by Gas Chromatography/Mass Spectrometry (GC/MS) ,” Journal of Pure and Applied Microbiology, vol. 4, no. 8, pp.2987-2998, 2014.
• [13] B. Baldaş, E. M. Altuner, “The antimicrobial activity of apple cider vinegar and grape vinegar, which are used as a traditional surface disinfectant for fruits and vegetables,” Communications Faculty of Sciences University of Ankara Series C Biology, vol. 27, no.1, pp.1-10, 2018.
• [14] J. M. Andrews, “BSAC standardized disc susceptibility testing method (version 6) ,” Journal of Antimicrobial Chemotherap, vol.60, pp. 20-41. 2003.
• [15] K. Canlı, A. Yetgin, I. Akata, E. M. Altuner, “In vitro Antimicrobial Activity of Angelica sylvestris Roots,” International Journal of Biological Sciences, vol.1, no.1, pp.1-7, 2016.
• [16] M. E. Bozyel, M. Şenturan, A. Benek, E. Bozyel Merdamert, K. Canlı, E. M. Altuner, “In Vitro Antimicrobial Activity Screening of Heliotropium europaeum Against Wide Range of Microorganisms and Multi Drug Resistant (MDR) Bacteria,” European Journal of Biomedical and Pharmaceutical Sciences, vol.6, no.3, pp.113-117, 2019.
• [17] Core R Team, R: A language and environment for statistical computing. R Foundation for Statistical Computing, https://www.R-project.org/ (last accession: 15.08.2019).
• [18] F. Savaroğlu, S. İlhan, C. Filik, İşcen, “An evaluation of the antimicrobial activity of some Turkish mosses,” Journal of Medicinal Plants Research, vol.5, no.14, pp. 3286-3292, 2011.
• [19] T. M. Karpiński, A. Adamczak, “Antibacterial activity of ethanolic extracts of some moss species” Herba Polonica, vol.63, no.3, pp.11-17, 2017.
• [20] Ö. Ertürk, H. Şahin, E. Ertürk, H. E. Hotaman, B. Koz, Ö. Özdemir, “The antimicrobial and antioxidant activities of extracts obtained from some moss species in Turkey,” Herba Polonica, vol.61, no.4, pp.52-65, 2015.
• [21] K. O. Olasoji, A. M. Makinde, B. A. Akinpelu, O. A. Igbeneghu, “Antimicrobial Activity of Selected Mosses on Obafemi Awolowo University Campus, Ile-Ife, Nigeria,” Notulae Scientia Biologicae, vol.11, no.3, pp.462-466, 2019.