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The Distribution of Vascular Endothelial Growth Factor and its Receptors in Avian Proventriculus

Year 2017, Volume: 10 Issue: 2, 122 - 129, 25.12.2017

Hakan Sağsöz Berna Güney Saruhan , Mehmet Erdem Akbalık Uğur Topaloğlu , Muzaffer Aydın Ketani

Abstract

In this study, to gain a better understanding of the physiological roles of vascular endothelial growth factor (VEGF) and its receptors (flt1/fms, flk1/KDR), in the avian proventriculus were examined cellular localizations by immunohistochemistry. For this study, 10 avian (5 males and 5 females) were used. Tissue samples from proventriculus were fixed in 10% formol-alcohol for routine histological processing. Positive immunoreaction for VEGF and its receptors was determined in the luminal and gland epithelial cells, stromal, smooth muscle cells and endothelial and smooth muscle cells of blood vessels in the avian proventriculus. However, it was determined that VEGF and its receptors showed a very strong cytoplasmic expression in glandular epithelial cells. As a result, it is thought that VEGF and its receptors may play a critical role in the proliferation, differentiation, apoptosis and angiogenesis of non-endothelial cells in the avian proventriculus.

Keywords

chicken flt1/fms proventriculus VEGF

References

  • 1. Nasrin M, Siddiqi MNH, Masum MA, Wares MA. (2012). Gross and Histological Studies of Digestive Tract of Broilers during Postnatal Growth and Development. J Bangladesh Agril Univ. 10 (1): 69-77.
  • 2. De Verdal H, Mignon-Grasteau S, Jeulin C, et al. (2010). Digestive Tract Measurements and Histological Adaptation in Broiler Lines Divergently Selected for Digestive Efficiency. Poultry Sci. 89: 1955-1961.
  • 3. Liman N, Alan E, Kücükbayram G. (2010). The Differences between the Localizations of MUC1, MUC5AC, MUC6 and Osteopontin in Quail Proventriculus and Gizzard may be a Reflection of Functional Differences of Stomach Parts. J Anat. 217: 57-66.
  • 4. Tanyolaç A. (1993). Özel Histoloji. R Demir (editör). Palme Yayıncılık, Ankara.
  • 5. Erdogan S, Sagsoz H, Akbalik ME. (2012). Anatomical and Histological Structure of the Tongue and Histochemical Characteristics of the Lingual Salivary Glands in the Chukar Partridge (Alectoris chukar, Gray 1830). Brit Poultry Sci. 53: 307-315.
  • 6. Erdogan S, Iwasaki S. (2014). Function-related Morphological Characteristics and Specialized Structures of the Avian Tongue. Ann Anat. 196: 75-87.
  • 7. Sagsoz H, Erdogan S, Akbalik ME. (2013). Histomorphological Structure of the Palate and Histochemical Profiles of the Salivary Palatine Glands in the Chukar partridge (Alectoris chukar, Gray 1830). Acta Zool-Stockholm. 94: 382-391.
  • 8. Ferrara N, Gerber HP, Le Couter J. (2003). The Biology of VEGF and its Receptors. Nat Med. 9: 669-676.
  • 9. Takahashi RI, Tanaka S, Kitadai Y et al. (2003). Expression of Vascular Endothelial Growth Factor and Angiogenesis in Gastrointestinal Stromal Tumor of the Stomach. Oncology-Basel. 64 (3): 266-274.
  • 10. Zhang HI, Wu J, Meng L, Shou CC. (2002). Expression of Vascular Endothelial Growth Factor and its Receptors KDR and Flt-1 in Gastric Cancer Cells. World J Gastroentero. 8 (6): 994-998.
  • 11. Raica MI, Mogoantă L, Cîmpean AM et al. (2008). Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Intestinal Type Gastric Carcinoma. Rom J Morphol Embryo. 49 (1): 37-42.
  • 12. Yamamoto S, Yasui W, Kitadai Y et al. (1998). Expression of Vascular Endothelial Growth Factor in Human Gastric Carcinomas. Pathol Int. 48: 499-506.
  • 13. Xiaoxiang LI, Zhenwei JI, Yunlei MA et al. (2012). Expression of Hypoxia-Inducible Factor-1α, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-2 in Sacral Chordomas. Oncol Lett. 3 (6): 1268-1274.
  • 14. Aitkenhead M, Christ B, Eichmann A et al. (1998). Paracrine and Autocrine Regulation of Vascular Endothelial Growth Factor during Tissue Differentiation in the Quail. Dev Dynam. 212: 1-13.
  • 15. Lee S, Chen TT, Barber CL et al. (2007). Autocrine VEGF Signaling is Required for Vascular Homeostasis. Cell. 130: 691-703.
  • 16. Nakajima Y, Mironov V, Yamagishi T et al. (1997). Expression of Smooth Muscle Alpha-Actin in Mesenchymal Cells during Formation of Avian Endocardial Cushion Tissue: A Role for Transforming Growth Factor Beta3. Dev Dynam. 209: 296-309.
  • 17. Robert B, Zhao X, Abrahamson DR. (2000). Coexpression of Neuropilin-1, Flk1, and VEGF (164) in Developing and Mature Mouse Kidney Glomeruli. American Journal of Physiology. Am J Physiol-Renal. 279: 275-282.
  • 18. Villegas G, Lange-Sperandio B, Tufro A. (2005). Autocrine and Paracrine Functions of Vascular Endothelial Growth Factor (VEGF) in Renal Tubular Epithelial Cells. Kidney Int. 67: 449-457.
  • 19. Fan X, Krieg S, Kuo CJ et al. (2008). VEGF Blockade Inhibits Angiogenesis and Reepithelialization of Endometrium. FASEB J. 22: 3571-3580.
  • 20. Sagsoz H, Saruhan BG. (2011). The expression of vascular endothelial growth factor and its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bovine uterus during the sexual cycle and their correlation with serum sex steroids. Theriogenology. 75 (9): 1720-1734.
  • 21. Li W, Keller GA. (2000). VEGF Nuclear Accumulation Correlates with Phenotypical Changes in Endothelial Cells. J Cell Sci. 113: 1525-1534.
  • 22. Makanya AN, Hlushchuk R, Baum O et al. (2007). Microvascular Endowment in the Developing Chicken Embryo Lung. Am J Physiol- Lung C. 292: 1136-1146.
  • 23. Tae K, El-Naggar AK, Yoo E et al. (2000). Expression of Vascular Endothelial Growth Factor and Microvessel Density in Head and Neck Tumorigenesis. Clin Cancer Res. 6: 2821-2828.

Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü ve Reseptörlerinin Dağılımı

Year 2017, Volume: 10 Issue: 2, 122 - 129, 25.12.2017

Hakan Sağsöz Berna Güney Saruhan , Mehmet Erdem Akbalık Uğur Topaloğlu , Muzaffer Aydın Ketani

Abstract

 Bu çalışmada kanatlı proventrikuluslarında
vasküler endotelyal büyüme faktörü (VEGF) ve reseptörlerinin (flt1/fms,
flk1/KDR) fizyolojik rollerini daha iyi anlamak için, immünohistokimya
kullanılarak hücresel lokalizasyonları incelendi. Çalışmanın materyalini 10
adet (5 erkek ve 5 dişi) tavuk oluşturdu. Proventrikulustan elde edilen doku
örnekleri rutin histolojik işlemler için % 10 formol-alkolde tespit edildi.
Kanatlı proventrikuluslarında luminal ve bez epitel hücreleri, stromal ve düz
kas hücreleri ile damarların endotel ve düz kas hücrelerinin VEGF ve
reseptörleri için pozitif immunreaksiyon gösterdiği belirlendi. Bununla
birlikte VEGF ve reseptörlerinin bez epitel hücrelerinde daha güçlü sitoplazmik
bir ekspresyon gösterdiği saptandı. Sonuç olarak VEGF ve reseptörlerinin kanatlı
proventrikulusundaki non-endotelyal hücrelerde çoğalma, farklılaşma, apopitozis
ve anjiogenezisin sağlanmasında kritik bir rolünün olabileceği düşünülmüştür.

Keywords

flt1/fms proventrikulus tavuk VEGF

References

  • 1. Nasrin M, Siddiqi MNH, Masum MA, Wares MA. (2012). Gross and Histological Studies of Digestive Tract of Broilers during Postnatal Growth and Development. J Bangladesh Agril Univ. 10 (1): 69-77.
  • 2. De Verdal H, Mignon-Grasteau S, Jeulin C, et al. (2010). Digestive Tract Measurements and Histological Adaptation in Broiler Lines Divergently Selected for Digestive Efficiency. Poultry Sci. 89: 1955-1961.
  • 3. Liman N, Alan E, Kücükbayram G. (2010). The Differences between the Localizations of MUC1, MUC5AC, MUC6 and Osteopontin in Quail Proventriculus and Gizzard may be a Reflection of Functional Differences of Stomach Parts. J Anat. 217: 57-66.
  • 4. Tanyolaç A. (1993). Özel Histoloji. R Demir (editör). Palme Yayıncılık, Ankara.
  • 5. Erdogan S, Sagsoz H, Akbalik ME. (2012). Anatomical and Histological Structure of the Tongue and Histochemical Characteristics of the Lingual Salivary Glands in the Chukar Partridge (Alectoris chukar, Gray 1830). Brit Poultry Sci. 53: 307-315.
  • 6. Erdogan S, Iwasaki S. (2014). Function-related Morphological Characteristics and Specialized Structures of the Avian Tongue. Ann Anat. 196: 75-87.
  • 7. Sagsoz H, Erdogan S, Akbalik ME. (2013). Histomorphological Structure of the Palate and Histochemical Profiles of the Salivary Palatine Glands in the Chukar partridge (Alectoris chukar, Gray 1830). Acta Zool-Stockholm. 94: 382-391.
  • 8. Ferrara N, Gerber HP, Le Couter J. (2003). The Biology of VEGF and its Receptors. Nat Med. 9: 669-676.
  • 9. Takahashi RI, Tanaka S, Kitadai Y et al. (2003). Expression of Vascular Endothelial Growth Factor and Angiogenesis in Gastrointestinal Stromal Tumor of the Stomach. Oncology-Basel. 64 (3): 266-274.
  • 10. Zhang HI, Wu J, Meng L, Shou CC. (2002). Expression of Vascular Endothelial Growth Factor and its Receptors KDR and Flt-1 in Gastric Cancer Cells. World J Gastroentero. 8 (6): 994-998.
  • 11. Raica MI, Mogoantă L, Cîmpean AM et al. (2008). Immunohistochemical Expression of Vascular Endothelial Growth Factor (VEGF) in Intestinal Type Gastric Carcinoma. Rom J Morphol Embryo. 49 (1): 37-42.
  • 12. Yamamoto S, Yasui W, Kitadai Y et al. (1998). Expression of Vascular Endothelial Growth Factor in Human Gastric Carcinomas. Pathol Int. 48: 499-506.
  • 13. Xiaoxiang LI, Zhenwei JI, Yunlei MA et al. (2012). Expression of Hypoxia-Inducible Factor-1α, Vascular Endothelial Growth Factor and Matrix Metalloproteinase-2 in Sacral Chordomas. Oncol Lett. 3 (6): 1268-1274.
  • 14. Aitkenhead M, Christ B, Eichmann A et al. (1998). Paracrine and Autocrine Regulation of Vascular Endothelial Growth Factor during Tissue Differentiation in the Quail. Dev Dynam. 212: 1-13.
  • 15. Lee S, Chen TT, Barber CL et al. (2007). Autocrine VEGF Signaling is Required for Vascular Homeostasis. Cell. 130: 691-703.
  • 16. Nakajima Y, Mironov V, Yamagishi T et al. (1997). Expression of Smooth Muscle Alpha-Actin in Mesenchymal Cells during Formation of Avian Endocardial Cushion Tissue: A Role for Transforming Growth Factor Beta3. Dev Dynam. 209: 296-309.
  • 17. Robert B, Zhao X, Abrahamson DR. (2000). Coexpression of Neuropilin-1, Flk1, and VEGF (164) in Developing and Mature Mouse Kidney Glomeruli. American Journal of Physiology. Am J Physiol-Renal. 279: 275-282.
  • 18. Villegas G, Lange-Sperandio B, Tufro A. (2005). Autocrine and Paracrine Functions of Vascular Endothelial Growth Factor (VEGF) in Renal Tubular Epithelial Cells. Kidney Int. 67: 449-457.
  • 19. Fan X, Krieg S, Kuo CJ et al. (2008). VEGF Blockade Inhibits Angiogenesis and Reepithelialization of Endometrium. FASEB J. 22: 3571-3580.
  • 20. Sagsoz H, Saruhan BG. (2011). The expression of vascular endothelial growth factor and its receptors (flt1/fms, flk1/KDR, flt4) and vascular endothelial growth inhibitor in the bovine uterus during the sexual cycle and their correlation with serum sex steroids. Theriogenology. 75 (9): 1720-1734.
  • 21. Li W, Keller GA. (2000). VEGF Nuclear Accumulation Correlates with Phenotypical Changes in Endothelial Cells. J Cell Sci. 113: 1525-1534.
  • 22. Makanya AN, Hlushchuk R, Baum O et al. (2007). Microvascular Endowment in the Developing Chicken Embryo Lung. Am J Physiol- Lung C. 292: 1136-1146.
  • 23. Tae K, El-Naggar AK, Yoo E et al. (2000). Expression of Vascular Endothelial Growth Factor and Microvessel Density in Head and Neck Tumorigenesis. Clin Cancer Res. 6: 2821-2828.
There are 23 citations in total.

Details

Journal Section Research
Authors

Hakan Sağsöz This is me

Berna Güney Saruhan

Mehmet Erdem Akbalık This is me

Uğur Topaloğlu

Muzaffer Aydın Ketani This is me

Publication Date December 25, 2017
Acceptance Date December 13, 2017
Published in Issue Year 2017 Volume: 10 Issue: 2

Cite

APA Sağsöz, H., Güney Saruhan, B., Akbalık, M. E., Topaloğlu, U., et al. (2017). Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü ve Reseptörlerinin Dağılımı. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 10(2), 122-129.
AMA Sağsöz H, Güney Saruhan B, Akbalık ME, Topaloğlu U, Ketani MA. Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü ve Reseptörlerinin Dağılımı. Dicle Üniv Vet Fak Derg. December 2017;10(2):122-129.
Chicago Sağsöz, Hakan, Berna Güney Saruhan, Mehmet Erdem Akbalık, Uğur Topaloğlu, and Muzaffer Aydın Ketani. “Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü Ve Reseptörlerinin Dağılımı”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 10, no. 2 (December 2017): 122-29.
EndNote Sağsöz H, Güney Saruhan B, Akbalık ME, Topaloğlu U, Ketani MA (December 1, 2017) Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü ve Reseptörlerinin Dağılımı. Dicle Üniversitesi Veteriner Fakültesi Dergisi 10 2 122–129.
IEEE H. Sağsöz, B. Güney Saruhan, M. E. Akbalık, U. Topaloğlu, and M. A. Ketani, “Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü ve Reseptörlerinin Dağılımı”, Dicle Üniv Vet Fak Derg, vol. 10, no. 2, pp. 122–129, 2017.
ISNAD Sağsöz, Hakan et al. “Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü Ve Reseptörlerinin Dağılımı”. Dicle Üniversitesi Veteriner Fakültesi Dergisi 10/2 (December 2017), 122-129.
JAMA Sağsöz H, Güney Saruhan B, Akbalık ME, Topaloğlu U, Ketani MA. Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü ve Reseptörlerinin Dağılımı. Dicle Üniv Vet Fak Derg. 2017;10:122–129.
MLA Sağsöz, Hakan et al. “Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü Ve Reseptörlerinin Dağılımı”. Dicle Üniversitesi Veteriner Fakültesi Dergisi, vol. 10, no. 2, 2017, pp. 122-9.
Vancouver Sağsöz H, Güney Saruhan B, Akbalık ME, Topaloğlu U, Ketani MA. Kanatlı Proventrikulusunda Vasküler Endotel Büyüme Faktörü ve Reseptörlerinin Dağılımı. Dicle Üniv Vet Fak Derg. 2017;10(2):122-9.
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