Konya Merinosu Koyunlarda Gebelik İlişkili Glikoproteinlerin Gebelikteki Plazma Profili ve Erken Gebelik Tanısında Kullanılabilirliği

Year 2018, Volume: 11 Issue: 2, 77 - 82, 31.12.2018

Uğur Uçar Mehmet Köse , Mehmet Osman Atlı

Abstract

Gebelik ile ilişkili glikoproteinler (PAGs)
koyunları da kapsayan ruminantlarda gebelikte trofoblastik hücrelerden üretilmektedir.
Sunulan bu çalışmada Konya Merinosu koyunlarda erken gebelik ve tüm gebelik
boyunca kan plazmasındaki PAGs konsantrasyonunun belirlenmesi amaçlandı. Toplam
16 baş gebe koyun kullanıldı. Çalışmanın birinci aşamasında (gebelik boyunca
PAGs profilinin izlenmesi) 8 baş koyundan gebeliğin 0, 55, 85, 113 ve 145.
günlerinde kan plazması toplandı. İkinci aşamada (erken gebelikteki PAGs
profilinin belirlenmesi) ise gebeliğin 18, 21, 25, 28 ve 35. günlerinde kan
plazma örnekleri toplandı. Elde edilen plazma örneklerinde PAGs değerleri
ticari (Sheep Pregnancy Associated Glycoproteins) ELISA kiti ile ölçüldü.
Sonuçlar değerlendirildiğinde gebeliğin 0. gününde plazmada bazal seviyede PAGs
olduğu tespit edilmiştir (14,99±1,63 ng/ml). Birinci aşamadaki veriler;
gebeliğin 0. günü ile kıyaslandığında; gebeliğin 55, 85, 113 ve 145.
günlerindeki değerleri arasında istatistikî olarak önemli bir fark tespit
edilmiştir (p<0,05). İkinci aşamada ise gebeliğin 35. gününde plazma PAGs
değerinin 18. gün ile kıyaslandığında önemli ölçüde arttığı tespit edilmiştir
(p<0,05). Elde edilen sonuçlar değerlendiğinde Konya Merinosu koyunlarda
gebelik boyunca plazma PAGs düzeyinin sürekli artan bir profil çizdiği
görülmektedir. Yine bu sonuçlara göre çalışmada kullanılan PAGs kiti ile Konya
Merinosu koyunlarda en erken 35. günde gebelik teşhisi yapılabileceği
söylenebilir.

Keywords

Gebelik, Gebelik Teşhisi, Gebelik ile İlişkili Glikoproteinler, Koyun

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