Ratlarda Karbon Tetraklorür ile Oluşturulan Kronik Karaciğer Hasarı Üzerine Kabak Çekirdeği Yağının Etkisi ve Kaspaz Aktivitesi ile Hepatik Apoptozisin Belirlenmesi

Year 2020, Volume: 13 Issue: 1, 24 - 32, 30.06.2020

Ayhan Atasever , Elife Sayar , Görkem Ekebaş , Meryem Şentürk , Meryem Eren

Abstract

Karbon tetraklorür (CCl4) ile kronik karaciğer hasarı oluşturulan ratlarda kabak çekirdeği yağının (KÇY) lezyonlar üzerine koruyucu etkisi, hücresel apoptozda kaspaz 3 aktivasyonu, serum Alanin Amino Transferaz (ALT) aktivitesi, trigliserit, total protein, albümin, total kolesterol düzeyleri ile karaciğer malondialdehit (MDA) ve total antioksidan kapasitesi (TAK) değerlendirildi. Çalışmada 48 adet rat, 12 hayvanlı 4 gruba ayrıldı. Grup I kontrol; Grup II ise CCl4 çalışma grubu olarak belirlendi. Grup III’e 4 ml/kg KÇY, 12 hafta gavajla verildi. Grup IV’e 4 hafta gavaj şeklinde KÇY verildi, takiben 8 hafta her gün 4 ml/kg KÇY ve intraperitoneal 0,2 ml/kg CCl4, haftada iki kere uygulandı. Grup III’de normal karaciğer dokusu gözlenirken; Grup II’de hepatositlerde yoğun makro ve mikroveziküler yağlanma, parankimde hücre infiltrasyonları, bağ doku artışına bağlı pseudolobulasyon formları görüldü. Grup IV’de oluşan lezyonların histopatolojisi Grup II ile benzerdi. Kontrol gruplarına göre çalışma gruplarındaki kaspaz 3 aktivitesi arttı. Grup II’de artan serum ALT aktivitesi (P<0,001), Grup IV’de değişiklik göstermedi. Grup II’de serum total protein ve albümin düzeyleri istatistiki önemde düşerken (P<0,001), Grup IV’de bu değerlerde sayısal azaldı. Grup IV’de yüksek olan serum total kolestrol düzeyleri KÇY’dan etkilenmedi. Grup II’de serum trigliserit düzeyleri önemli düzeyde artarken (P<0,001), Grup IV’de sayısal olarak düştü. Grup IV’de KÇY uygulanması, MDA değerlerini önemli düzeyde düşürürken (P<0,001), Total antioksidan kapasitesi yönünden ise gruplar arasında fark saptanamadı (P>0,05). Sonuç olarak; CCl4 ile birlikte KÇY verilen Grup IV’de karaciğer hasarı üzerinde bitkinin antioksidan özelliklerinin biyokimyasal veriler ve histopatolojik lezyonlar üzerine iyileştirici yönden anlamlı bir etkisi gözlenmedi.

Keywords

Histopatoloji, İmmunhistokimya, Karbon tetraklorür, Kabak çekirdeği yağı, Rat

Supporting Institution

Erciyes Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından desteklenmiş ve yüksek lisans tezinden üretilmiştir.

Project Number

TYL-2018-8449

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