Pre- and post-exercise ADAMTS-4 and ADAMTS-5 Levels in Concur Horses

Year 2020, Volume: 13 Issue: 2, 99 - 103, 31.12.2020

Sinan Kandır , Cenk Er , Serdar Karakurt

https://doi.org/10.47027/duvetfd.738477

Abstract

A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteinase family play an important role in many physiological and physiopathological processes such as the maintenance of locomotor system health in sport horses. In this study, we aimed to determine the changes of ADAMTS-4 and ADAMTS-5 levels in concour horses before and after exercise.
The Oldenburg and Selle Français horse-breed types which are healthy, 6-15 years old, around 650-750 kg, and distinct genders were used (n=10). Following the physical examinations, the horses were subjected to 50 minutes of regular exercise program. Blood samples were collected into anticoagulant-free tubes in order to determine ADAMTS-4 and ADAMTS-5 mRNA expression and ELISA levels before and after exercise.
There were no differences were observed statistically on ADAMTS-4, neither mRNA expression in spite of 25% downregulated, nor at the ELISA levels. On the other hand, ADAMTS-5 mRNA expression was upregulated 3.88 fold (p<0.01), as well ELISA level significantly increased as 1.2 fold (p<0.01).

Keywords

ADAMTS, Aggrecanase, Equine Exercise, Metalloproteinase

Supporting Institution

Çukurova Üniversitesi BAP

Project Number

CUBAP-TSA-2017-9288

Thanks

This work was supported by the Cukurova University Scientific Research Project Unit (grant number CUBAP-TSA-2017-9288). The part of this project was presented in FEPS2017 Congress in Vienna and abstracted in Acta Physiologica vol.221;S713, p:195 by the support of CUBAP [TED-2017-9445].

Konkur Atlarında Egzersiz Öncesi ve Sonrası ADAMTS-4 ve ADAMTS-5 Düzeyleri

Abstract

A disintegrin-like and metalloproteinase with thrombospondin motifs (ADAMTS) proteinaz ailesi, spor atlarında lokomotor sistem sağlığının korunması gibi birçok fizyolojik ve fizyopatolojik olayda önemli rol oynamaktadır. Bu çalışmada, yarış atlarında egzersiz öncesi ve sonrasındaki ADAMTS-4 ve ADAMTS-5 düzeylerinin belirlenmesi amaçlanmıştır.
Bu amaçla, sağlıklı, 6-15 yaşları arasında, 650-750 kg ağırlığında, farklı cinsiyetlerden, Oldenburg ve Selle Français ırkı yarış atları (n=10) kullanıldı. Atların fiziksel muayeneleri ardından, 50 dakikalık standart egzersiz programı uygulandı. Egzersiz öncesi ve sonrasında ADAMTS-4 ve ADAMTS-5 mRNA ekspresyonlarını ve ELİSA düzeyleri ölçülmek üzere antikoagulansız tüplere kan örnekleri alındı.
ADAMTS-4 mRNA ekspresyonu %25 oranında azalmasına rağmen istatistiki olarak anlamlı bulunmadı ve ELİSA düzeyinde de değişim gözlenmedi. ADAMTS-5 mRNA ekspresyonu 3.88 kat arttı (p<0.01) ve ELİSA düzeyi de anlamlı olarak 1.2 kat arttı (p<0.01).
Sonuç olarak, serum ADAMTS-5 düzeylerindeki artışın, atlarda eklem hastalıklarının da potansiyel biyolojik belirteçlerinden biri olabileceği ve etkilerini netleştirmek için klinik bulgularla desteklenen daha detaylı araştırmaların yapılması gerektiği kanısına varıldı.

Keywords

ADAMTS, Agrekanaz, At Egzersiz, Metalloproteinaz

Project Number

CUBAP-TSA-2017-9288

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