Üst Solunum Sistemi Problemli Kedilerde Feline Herpesvirus 1 Enfeksiyonun PCR Yöntemi ile Araştırılması
Year 2021,
Volume: 14 Issue: 2, 142 - 144, 31.12.2021
Aynur Şimşek
,
Nazan Baksi
Abstract
Bu çalışmada, kedilerin üst solunum yolu enfeksiyonunda Feline herpesvirus 1 (FHV-1)’in rolünün belirlenmesi amaçlandı. Çalışma kapsamında 45 hasta kedinin klinik muayaneleri yapılarak FHV-1 enfeksiyonu yönünden değerlendirildi. FHV-1 enfeksiyonunun klinik bulgularını (hapşırma, gözyaşı ve burun akıntısı, salivasyon) gösterip aşı ve herhangi bir tedavi uygulanmamış 20 kedi çalışmaya dahil edildi. Kedilerden alınan orofarengeal ve konjunktival swap örneklerinde polymerase chain reaction (PCR) yöntemi ile FHV-1 varlığı araştırıldı. PCR analizlerinde kedilerin 13’ünün (%65) FHV-1 yönünden pozitif olduğu tespit edildi. Sonuç olarak, Diyarbakır bölgesindeki kedilerin üst solunum yolu enfeksiyonunda FHV-1’in önemli rol aldığı ortaya konuldu.
References
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Year 2021,
Volume: 14 Issue: 2, 142 - 144, 31.12.2021
Aynur Şimşek
,
Nazan Baksi
References
- 1. Sykes JE. (2014). Pediatric Feline Upper Respiratory Disease. Vet Clin Small Anim. 44: 331-342.
- 2. Albay MK. (2018). Kedi Gribi: Kedilerin Üst Solunum Sistemi Hastalık Kompleksi Turkiye Klinikleri J Vet Sci Intern Med-Special Topics. 4(1): 29-34.
- 3. Sykes JE, Allen JL, Studdert VP, Browning GF. (2001). Detection of Feline Calicivirus, Feline Herpesvirus 1 and Chlamydia Psittaci Mucosal Swabs by Multiplex RT-PCR/PCR. Vet Microbiol. 81: 95-108.
- 4. Yıldırım Y, Küçük A. (2018). Felide Herpes Virus-1 Enfeksiyonu. Etlik Vet Mikrobiyol Derg. 29(1): 76-81.
- 5. Gaskel R, Dawson S, Radford A, Thiry E. (2007). Feline herpesvirus. Vet Res. 38: 337-354.
- 6. Henzel A, Brum MCS, Lautert C, Martins M, Lovato LT, Weiblen R. (2012). Isolation and Identification of Feline Calicivirus and Feline Herpesvirus in Southern Brazil. Braz J Microbiol. 43(2): 560-568.
- 7. Albay MK. (2018). Kedi Gribi: Kedilerin Üst Solunum Sistemi Hastalık Kompleksi. Turkiye Klinikleri J Vet Sci Intern Med-Special Topics. 4(1): 29-34.
- 8. Bilge Dağalp S, Doğan F, Farzani TA, Babaoğlu AR, Acar Kırmızı G, Çabalar M. (2019). Solunum sistemi problemli kedilerde Feline Herpesvirus 1 (FHV-1) ve Feline Calicivirus varlığının moleküler olarak araştırılması. Eurasian J Vet Sci. 35(3): 131-138.
- 9. Küçük A, Yıldırım Y. (2018). Felide Herpesvirus–1 Enfeksiyonu. Etlik Vet Mikrobiyol Derg. 29(1): 76-81.
- 10. Kang BT, Park HM. (2008). Prevalence of Feline Herpesvirus 1, Feline Calicivirus and Chlamydophila Felis in Clinically Normal Cats at a Korean Animal Shelter. Journal of Veterinary Science. 9(2): 207-209.
- 11. Gaskell RM, Radford A, Dawson S. (2012). Feline Respiratory Disease. In: Infectious Disease of Dog and Cats. Green CE (ed.). 4th ed. pp. 151-162. Saunders Elsevier, Missouri, USA.
- 12. Küçük A, Sağ N, Çakır C, Acar G, Yıldırım Y, Ataseven VS. (2017). Sağlıklı görünüşlü ve solunum sistemi problemli barınak kedilerinde Feline Herpesvirus Tip 1 (FeHV-1) Enfeksiyonu. Erciyes Üniv Vet Fak Derg. 14(1): 25-30.
- 13. Litster A, Wu CC, Leutenegger CM. (2015). Detection of Feline Upper Respiratory Tract Disease Pathogens Using A Commercially Available Real-Time PCR test. The Veterinary Journal. 206: 149-153.
- 14. Sykes JE, Browning GF, Anderson G, Studdert VP, Smith HV. (1997). Differential Sensitivity of Culture and The Polymerase Chain Reaction for Detection of Feline herpesvirus 1 in Vaccinated and Unvaccinated Cats. Arch Virol. 142: 65-74.
- 15. Walter J, Foley P, Yason C, Vanderstichel R, Muckle A. (2020). Prevalence of Feline Herpesvirus-1, Feline Calicivirus, Chlamydia Felis, and Bordetella Bronchiseptica in a Population of Shelter Cats on Prince Edward Island. Canadian Journal of Veterinary Research, 84(3): 181-188.
- 16. Maggs DJ. (2005). Update on Pathogenesis, Diagnosis, and Treatment of Feline Herpesvirus Type 1. Clin Tech Small Anim Pract. 20: 94-101.
- 17. Fernandez M, Manzanilla EG, Lloret A, León M, Thibault JC. (2017). Prevalence of Feline Herpesvirus-1, Feline Calicivirus, Chlamydophila Felis and Mycoplasma Felis DNA and Associated Risk Factors in Cats in Spain with Upper Respiratory Tract Disease, Conjunctivitis and/or Gingivostomatitis. J Feline Med Surg. 19(4): 461-469.
- 18. Najafi H, Madadgar O, Jamshidi S, Langeroudi AG, Lemraski MD. (2014). Molecular and Clinical Study on Prevalence of Feline Herpesvirus Type 1 and Calicivirus in Correlation with Feline Leukemia and Immunodeficiency Viruses. Vet Res Forum. 5(4): 255-261.
- 19. Helps CR, Lait P, Damhuis A, et al. (2005). Factors Associated with Upper Respiratory Tract Disease Caused by Feline herpesvirus, Feline calicivirus, Chlamydophila felis and Bordotella bronchiseptica in Cats: Experience from 218 European Catteries. Veterinary Record.156: 669-673.
- 20. Bilge Dağalp S, Akça Y. (2004). Detection of Feline Herpesvirus-1 From Domestic Cats with or without Respiratory Symptoms. Indian Vet J. 81(1): 11-15.
- 21. Bilge Dağalp S, Doğan F, Farzani TA, Babaoğlu AR, Acar Kırmızı G, Çabalar M. (2019). Solunum Sistemi Problemli Kedilerde Feline Herpesvirus 1 (Fhv-1) ve Feline Calicivirus Varlığının Moleküler Olarak Araştırılması. Eurasian Vet Sci. 35(3): 131-138.
- 22. Karapınar Z, Dinçer E, Ataseven VS, Karaca M. (2014). Felid Herpesvirus-1 infection in Van Cats with Conjunctivitis. YYU Vet Fak Derg. 25(1): 15-17.
- 23. Bayraktar E, Yılmaz H. (2020). Molecular Detection and Clinical Aspects of Feline Herpesvirus-1, Feline İmmunodeficiency Virus and Feline Leukemia Virus in Cats in Istanbul, Turkey. Pak Vet J. 40(2): 249-252.
- 24. Abayli H, Can Sahna K, Ozbek R, Aslan O, Tonbak S, Bulut H. (2021). Detection and molecular characterisation of feline viruses from swab samples. Acta Veterinaria Hungarica. 69 (2): 194-203.