Oestrus ovis Larvaları ile Enfekte İvesi Koyunlarda Lipit Peroksidasyonun, Toplam Antioksidan Kapasitesinin ve Bazı Hematolojik Parametrelerin Değerlendirilmesi

Year 2023, Volume: 16 Issue: 2, 80 - 84, 31.12.2023

Duygu Neval Sayın İpek , Polat İpek , Aynur Şimşek , Akın Koçhan , Ömer Faruk Katanalp

https://doi.org/10.47027/duvetfd.1264334

Abstract

Oestrosis, koyun ve keçilerin burun boşluğu ve frontal sinuslarına yerleşip gelişen Oestrus ovis sineğinin birinci, ikinci ve üçüncü dönem larvalarının neden olduğu bir burun miyazdır. Bu çalışmanın amacı Oestrus ovis larvaları ile doğal enfeste koyunlarda oestrosisin lipid peroksidasyon, total antioksidan kapasite ve bazı hematolojik parametreler üzerine olan etkisini araştırmaktır. Bu çalışma kırk koyun üzerinde yürütüldü. Oestrosisin moleküler tanısı için koyunların her iki burun deliğinden steril pamuklu swaplar ile mukus numuneleri alındı. Lipid perokidasyonu ve total antioksidan kapasite seviyeleri ile total lökosit sayısı, eritrosit sayısı, hematokrit değer ve hemoglobin konsantrasyonunun belirlenmesi için koyunların vena jugularislerinden kan örnekleri alındı. 40 swap örneğinden 35’inin semi-nested PCR yöntemi kullanılarak oestrosis pozitif olduğu tespit edildi. Enfeste olduğu tespit edilen hayvanlar burun akıntısının şiddetine göre iki gruba ayrıldı (grup 1: hafif burun akıntısı olanlar; grup 2: şiddetli burun akıntısı olanlar). Grup 1 ve 2’de serum lipid peroksidasyonu artışının kontrol grubuna göre istatiksel olarak önemli olduğu belirlendi (p˂0,05). Grup 1 ve grup 2 kontrol grubu ile kıyaslandığında total antioksidan kapasitede azalma görülmesine rağmen, kontrol grubu ile aralarındaki farkın istatiksel olarak önemli olmadığı belirlendi (p>0,05). Hematolojik parametrelerde gruplar arasında önemli farklılık bulunmadı (p>0,05). Sonuç olarak, koyunlarda Oestrus ovis larvalarının neden olduğu doku hasarı, lipid peroksidasyonun artışına neden olabileceği kanısına varıldı.

Keywords

Hematolojik, Lipid Peroksidasyon, Oestrosis, Total Antioksidan Kapasite

Evaluation of Lipid Peroxidation, Total Antioxidant Capacity and Some Hematological Parameters in Awessi Sheep with Oestrosis

Abstract

Oestrosis, a nasal myiasis of sheep and goats, is caused by the larvae of Oestrus ovis, which develop from first to third stage larva in the nasal cavity and frontal sinuses of sheep and goats. The aim of this study was to investigate the effect of oestrosis on lipid peroxidation, total antioxidant capacity and some hematological parameters in sheep naturally infested with Oestrus ovis larvae. The study was carried out on forty sheep. For molecular diagnosis of oestrosis, mucus samples were collected from both the nasal meatuses of sheep with sterile cotton swabs. Blood samples were collected from vena jugularis of sheep for determination of lipid peroxidation and total antioksidant capacity levels, and white blood cell count, red blood cell count, packed cell volume and hemoglobin concentration. Diagnosis of oestrosis was made by semi-nested PCR. The 35 out of 40 swabs samples were found positive for oestriosis. The infested animals were divided into two groups according to severity of nasal discharge (group1: with less nasal discharge; group 2: with severe nasal discharge). It was determined that the elevation in serum lipid peroxidation levels established a significant difference between the control group and groups 1, 2 (p˂0,05). Althought decreased were seen in total antioxidant capacity, there were no significant differences between the control group, group 1 and 2 (p>0,05). Significant differences were not determined in hematological parameters between groups (p>0,05). In conclusion, Oestrus ovis larvae can cause tissue damage which result to increasing in lipid peroxidation in sheep.

Keywords

Hematological, Lipid Peroxidation, Oestrosis, Total Antioxidant Capacity

References

• 1. Abo-Shehada MN, Batainah T, Abuharfeil N, Torgerson PR (2003). Oestrus ovis Larval Myiasis Among Goats in Northern Jordan. Prev Vet Med. 59(1-2): 13-19.
• 2. Alcaide M, Reina D, Sánchez J, Frontera E, Navarrete I. (2003). Seasonal Variations in The Larval Burden Distribution of Oestrus ovis in Sheep in The Southwest of Spain. Vet Parasitol. 118(3-4): 235-241.
• 3. Dorchies P, Bergeaud JP, Tabouret G, Duranton C, Prevot F, Jacquiet P. (2000). Prevalence and Larval Burden of Oestrus ovis (Linné 1761) in Sheep And Goats in Northern Mediterranean Region of France. Vet Parasitol. 88(3-4):269-273.
• 4. Papadopoulos E, Chaligiannis I, Morgan ER. (2010). Epidemio-logy of Oestrus ovis L.(Diptera: Oestridae) Larvae in Sheep and Goats in Greece. Small Rum Res. 89(1):51-56.
• 5. Uslu U, Dik B. (2006). Prevalence and Intensity of Oestrus ovis in Akkaraman Sheep in the Konya Region of Turkey. Med Vet Entomol. 20(3): 347-349.
• 6. Özdal N, Tanritanir P, Ilhan F, Değer S. (2016). The Prevalence of Ovine Oestrosis (Oestrus ovis Linnaeus, 1761, Diptera: Oestri-dae) and Risk Factors in Eastern Turkey. Veterinarski Ar-hiv. 86(3):323-333.
• 7. Scala A, Solinas G, Citterio CV, Kramer LH, Genchi C. (2001). Sheep Oestrosis (Oestrus ovis Linné 1761, Diptera: Oestridae) in Sardinia, Italy. Vet Parasitol. 102(1-2):133-141.
• 8. Alcaide M, Reina D, Frontera E, Navarrete I. (2005). Epidemio-logy of Oestrus ovis (Linneo, 1761) Infestation in Goats in Spain. Vet Parasitol. 130(3-4): 277-284.
• 9. Mozaffari AA, Shojaeepour S, Ghahremani Ghareh Cheshmeh S. (2013). High Mortality Rate Due to False Gid in a Sheep Herd. ISRN Veterinary Science. 2013:650358 10. Blood DC, Radostits OM, Arundel JH, Gay CC. (1989). Veterinary Medicine: a Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses. 7th ed. Bailliere Tindall, London.
• 11. Dorchies P, Duranton C, Jacquiet P. (1998). Pathophysiology of Oestrus ovis Infection in Sheep and Goats: a Review. Vet Rec. 142(18): 487-489.
• 12. Angulo-Valadez CE, Reyes-Becerril MC, Romero GM, Cepeda-Palacios R, López-Aguilar DR, Zenteno T, Ascencio F. (2011). An-tioxidant Enzymes in Erythrocytes from Goats seropositive to the Sheep Nose Bot Fly (Oestrus ovis L. Diptera: Oestridae) Infection. Vet Parasitol. 183(1-2): 140-145.
• 13. Tabouret G, Bret-Bennis L, Dorchies P, Jacquiet P. (2003). Serine Protease Activity in Excretory-Secretory Products of Oestrus ovis (Diptera: Oestridae) Larvae. Vet Parasitol. 114:305–314.
• 14.Attia MM, Soliman SM, Salaeh NMK. (2019). Advanced and Ra-pid Serodiagnosis of Oestrosis (Oestrus ovis; Diptera: Oestridae) in Sheep Using İndirect and Dot-ELISA. Jordan J Biol Sci. 12(3):275–281.
• 15.Sandeman RM. (1996). Immune Responses to Mosquitoes and Flies. In: Wikel SK (ed) The Immunology of Host-Ectoparasitic Arthropod Relationship. CAB International, Wallingford. pp. 175–203.
• 16.Erenel G, Erbaş D, Akıcıoğlu A. (1992). Serbest Radikaller ve An-tioksidan Sistemler. Gazi Tıp Derg. 3(4): 243-250.
• 17.Wolff SP, Garner A, Dean RT. (1986). Free Radicals, Lipids and Protein Degradation. Trends Biochem Sci. 11(1): 27-31.
• 18. Köse K, Doğan P. (1992). Lipid Peroksidasyonu. Erciyes Tıp Derg. 1: 340-350.
• 19. Wood LG, Gibson PG, Garg ML. (2003). Biomarkers of Lipid Pe-roxidation, Airway Inflammation and Asthma. Eur Respir J. 21(1): 177-186.
• 20.Cross CE. (2003). The Antioxidant Milieu at Asthmatic Respira-tory Tract Surfaces: Commentary on the Article by Schock et al. on page 375. Pediat res. 53(3): 365-368.
• 21. Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. (1993). A Novel Method for Measuring Antioxidant Capacity and Its Application to Monitoring the Antioxidant Status in Pre-mature Neonates. Clin Scien. 84(4): 407-412.
• 22. Motoyama T, Okamoto K, Kukita I, Hamaguchi M, Kinoshita Y, Ogawa H. (2003). Possible Role of Increased Oxidant Stress in Multiple Organ Failure After Systemic Inflammatory Response Syndrome. Crit. Care Med. 31(4): 1048-1052.
• 23. Sayin Ipek DN, Altan S. (2017). Use of Semi-Nested PCR and Rhi-noscopy for the Diagnosis of Oestrosis. Small Rum Res. 150: 76-79.
• 24. Erel O. (2004). A Novel Automated Method to Measure Total Antioxidant Response Against Potent Free Radical Reacti-ons. Clin Biochem Clinical. 37(2): 112-119.
• 25. Gül Y, İssi M, Özer S. (2007). Oestrosis ve Coenurosis’e Bağlı Ola-rak Epileptoit Nöbet Gösteren Bir Koyun Sürüsünde Klinik ve Patolojik Gözlemler. F.Ü. Sağ. Bil. Derg. 21(4): 173-177.
• 26. Dönmez N, Uslu U, Atalay B. (2006). Coenurus cerebralis ve Oestrus ovis ile Enfekte Koyunlarda Bazı Hematolojik Paramet-reler. Vet Bil Derg. 22(3-4): 75-77.
• 27. Rezaei SA, Dalir-Naghadeh B. (2006). Evaluation of Antioxidant Status and Oxidative Stress in Cattle Naturally Infected with Theileria Annulata. Vet parasitol. 142(1-2): 179-186.
• 28. Kiral F, Karagenc T, Pasa S, Yenisey C, Seyrek K. (2005). Dogs with Hepatozoon Canis Respond to the Oxidative Stress by Inc-reased Production of Glutathione and Nitric Oxide. Vet Parasi-tol. 131(1-2): 15-21.
• 29. Aktas MS, Kandemir FM, Kirbas A, Hanedan B, Aydin MA. (2017). Evaluation of Oxidative Stress in Sheep Infected with Psoroptes ovis Using Total Antioxidant Capacity, Total Oxidant Status, and Malondialdehyde Level. J Vet Res. 61(2): 197-201
• 30. Camkerten I, Sahin T, Borazan G, Gokcen A, Erel O, Das A. ( (2009). Evaluation of Blood Oxidant/Antioxidant Balance in Dogs with Sarcoptic Mange. Vet Parasitol. 161(1): 106-109.
• 31. Amanvermez R, Çelik C. (2004). The Superoxide Dismutase in the Hydatid Cyst, Glutathione, Vitamin C, Total Antioxidant and Total Thiol Levels. T Klin J Med Sci. 24: 213-218.
• 32. Jacquiet P, Dorchies P. (2002). Towards a Lower Prevalence of Oestrus ovis Infections in Sheep in a Temperate Climate (South West France). Vet Res. 33(5): 449-453.
• 33. Attia MM, El-Gameel SM, Ismael E. (2020). Evaluation of Tumor Necrosis Factor-Alpha (TNF-Α); Gamma İnterferon (IFN-Γ) Ge-nes and Oxidative Stress in Sheep: immunological Responses In-duced by Oestrus ovis (Diptera: Oestridae) Infestation. J Parasit Dis. 44(2): 332–337.
• 34. Dönmez N, Keskin E. (2007). Coenurus cerebralis ve Oestrus ovis ile Enfekte Koyunlarda Bazı Biyokimyasal Parametreler. Vet Bil Derg. 23(1): 107-109.