Antibiotic Resistance of Some Probiotic Strains in Aquaculture

Year 2017, Volume: 3 Issue: 1, 22 - 26, 30.04.2017

Dilek Özcan Şükrü Önalan

Abstract

The term “probiotic” was firstly used to denominate microorganisms that have effects on other microorganisms. Antibiotics are used extensively because diseases are the most important factor limiting production and trade in aquaculture. Intensive use of antibiotics causes resistance to antibiotics, adverse effects on human health and many damages in the ecosystem.  In aquaculture, research on the use of probiotics to stabilize the bacterial populations in the water, reduce pathological bacterial load and improve water quality is increasing and the use of probiotics is widespread. Probiotics are single or mixed live microorganism cultures or metabolites there of which promote the development of the intestinal microflora of the host organisms, cause recovery and rapid growth in the oral cavity, gastrointestinal system, upper respiratory tract. On the other hand, probiotics help to detoxify potentially harmful compounds in foods and feed them by feeding them with amylases and proteases, digesting potentially inaccessible elements in the diet, stimulating the production of vitamins and the congestive immune system. For these reasons it is important to identify antibiotic resistant strains and use them in aquaculture. In this study, 13 antibiotics (Kanamycin-K30, Penicillin-P10, Ampicillin-AM10, Erythromycin-E15, Clindamycin-DA2, Rifampin-RA5, Methicillin-ME5, Enrofloxacin-ENR5, Florfenicol-FFC30, Ciprofloxacin-CIP5, Nitrofurantoin-F300, Methicillin-ME5, Trimethoprim/ Sulphamethoxzole-SXT25) and 4 probiotic bacteria (Lactobacillus rhamnosus, Lactobacillus casei, Lactobacillus fermentum, Lactobacillus paracasei) were used. As a consequence; the probiotic bacteria were determinated that L. rhamnosus was resistant to antibiotics of K30, P10, AM10, E15, DA2 and RA5; was intermediate T30 and then it was sensitive EN5, FFC30, CIP5, F300, ME5 and SXT25. L. fermentum was resistant to K30, AM10, DA2, T30, ME5 and RA5İ; was intermediate E15 and CIP5; and then it is sensitive to P10, ENR5, FFC30, F300 and SXT25. L. paracasei tested was resistant to K30, P10, AM10, E15, DA2 and RA5; was intermediate T30; and then it was sensitive to ENR5, FFC30, CIP5, F300, ME5 and SXT25. L. casei in probiotic bacteria used in the aquaculture was found resistant to antibiotics of K30, P10, AM10, E15, DA2, T30 and RA5 were sensitive, was intermediate ME5; it was sensitive to ENR5, FFC30, CIP5, F300 and SXT25.

Keywords

Probiotics, Antibiotic resistance, Fish Diseases, Aquaculture

References

• BALCÁZAR, J. L., DE BLAS, I., RUIZ-ZARZUELA, I. CUNNINGHAM, D., DANIEL VENDRELL, D. and MÚZQUIZ, J.L. "The role of probiotics in aquaculture." Veterinary microbiology 114.3 (2006): 173-186. BALTA, F., BALTA, Z. D., ÖZGÜMÜŞ, O. B., & ÇAĞIRGAN, H. (2016). Doğu Karadeniz Bölgesi’ndeki Gökkuşağı Alabalığı (Oncorhynchus mykiss) Çiftliklerinde Yersinia ruckeri’nin Portörlük Yönünden Tetkiki ve Antimikrobiyal Direncin Tespiti. Journal of Anatolian Environmental&Animal Sciences. Year: 1, No: 3, 2016 (72-76). CANNON, J. P., LEE, T. A., BOLANOS, J. T., and DANZIGER, L. H. "Pathogenic relevance of Lactobacillus: a retrospective review of over 200 cases." Eur. J. Clin. Microbiol. Infect. Dis. 24, 31–40. doi: 10.1007/s10096-004-1253-y (2005). CLSI, (2003). Performance standards for antimicrobial disk susceptibility test; 8th ed., Approved standard M2-A8., CLSI, Wayne, PA, USA. CORDERO, H., ESTEBAN, M. A., and CUESTA A. "Use of probiotic bacteria against bacterial and viral infections in shellfish and fish aquaculture." Sustainable aquaculture techniques, Murcia, Spain (2014). FAO. The State of World Fisheries and Aquaculture. Rome, Italy: FAO Fisheries and Aquaculture Department (2014). FLEGEL, T. W. Detection of major penaeid shrimp viruses in Asia, a historical perspective with emphasis on Thailand. Aquaculture, 258: 1–33 (2006). GATESOUPE, F. J. "The use of probiotics in aquaculture." Aquaculture 180.1 (1999): 147-165. IRIANTO, A., and AUSTIN, B. "Probiotics in aquaculture." Journal of fish diseases 25.11 (2002): 633-642. LILLY, D. M., and STILLWELL, H. R. "Probiotics: growth-promoting factors produced by microorganisms." Science 147.3659 (1965): 747-748. NCCLS (National Committee for Clinical Laboratory Standards). "Methods for antimicrobial disk susceptibility testing of bacteria isolated from aquatic animals." Approved report M42-R. National Committee for Clinical Laboratory Standards, Wayne, Pa. (2003). NIKOSKELAINEN, S., A.C., OUWEHAND, G. BYLUND, SEPPO SALMINEN, S. and MATTI LILIUS, E. "Immune enhancement in rainbow trout (Oncorhynchus mykiss) by potential probiotic bacteria (Lactobacillus rhamnosus)." Fish & shellfish immunology 15.5 (2003): 443-452. PANİGRAHİ, A., & AZAD, I. S. (2007). Microbial intervention for better fish health in aquaculture: the Indian scenario. Fish physiology and biochemistry, 33(4), 429-440. PILLAY, T. V. R. and KUTTY, M. N. Aquaculture: Principles and Practices. Oxford, UK: Wiley-Blackwell, 2005. QI, Z., et al. "Probiotics in aquaculture of China—current state, problems and prospect." Aquaculture 290.1 (2009): 15-21. QI, Z., ZHANG, X. H., BOON, N., & BOSSİER, P. (2009). Probiotics in aquaculture of China—current state, problems and prospect. Aquaculture, 290(1), 15-21. RINGØ, E., SCHILLINGER, U., and HOLZAPFEL W. "Antimicrobial activity of lactic acid bacteria isolated from aquatic animals and the use of lactic acid bacteria in aquaculture." Biology of growing animals 2 (2005): 418-453. ROSSI, F., DI RENZO, T, PREZIUSO, M., ZOTTA, T. and IACUMIN, L. "Survey of antibiotic resistance traits in strains of Lactobacillus casei/ paracasei/ rhamnosus." Ann Microbiol. (2015): 65:1763–1769. SHAO, Y., ZHANG, W., GUO, H., PAN, L., ZHANG, H. and SUN, T. "Comparative studies on antibiotic resistance in Lactobacillus casei and Lactobacillus plantarum." Food Control 50 (2015): 250-258. SHARMA, P., TOMAR, S.K., GOSWAMİ, P., SANGWAN, V. and SINGH, R. "Antibiotic resistance among commercially available probiotics." Food Research International 57 (2014) 176–195. TURGUT, E., DEVELI, N. and USTAOĞLU TIRIL, S. "Su ürünleri yetiştiriciliğinde probiyotiklerin kullanımı." Gaziosmanpaşa Üniversitesi Ziraat Fakültesi Dergisi (2007):2. VASEEHARAN, B. A. R. P., & RAMASAMY, P. (2003). Control of pathogenic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Letters in applied microbiology, 36(2), 83-87.