Investigation of Dynamic Thiol Disulfide Homeostasis in Acute Respiratory Failure Patients in Intensive Care Unit

Year 2021, Volume: 2 Issue: 2, 164 - 175, 29.06.2021

Zeynep Apaydın Hamit Yıldız , Fatma Samin Belgin Alaşehirli

Abstract

Background/aim:Thiols are antioxidant agents which belong to mercaptan group consisting of sulfur and carbon containing sulfhydryl (-SH). Thiol levels, in the case of elevated oxidative stress, are lowered by their use in neutralization of reactive oxygen molecules.
Materials and methods:It was aimed to investigate thiol and disulphide blood levels or thiol/disulphide ratio as a marker of total oxidant status which plays a role in the pathogenesis of inflammatory diseases that may cause respiratory failure.
Results:The study included 98 patients (58 males, 40 females) who have partial oxygen pressure (PaO2)<60 mmHg or partial carbon dioxide pressure (PaCO2)>45 mmHg in arterial blood in the intensive care unit and 98 healthy volunteers. Total thiol and native thiol levels were measured by spectrophotometric method. Total thiol (270 ± 99.81), native thiol (203.90 ± 103.41) and disulphide (33.10±12.42) levels of the patient group were significantly lower (p <0.001) than total thiol (423.62 ± 70.3), native thiol (307.13 ± 57.73) and disulphide (58.24±27.21) of the control group levels. There is no significant difference between native thiol / total thiol, disulphide/ total thiol and disulphide /native thiol ratios.
Conclusion:Thiol and disulphide blood levels or thiol / disulphide ratio can guide us as a prognostic test in acute respiratory failure patients.

Keywords

Native thiol, Oxidative stress, Respiratory failure, Thiol/disulphide homeostasis, Total thiol

Project Number

TF.YLT.18.24

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