Safranal'ın Servikal Kanseri C-4 I Hücre Hattı Üzerindeki Antiproliferatif Etkisinin Değerlendirilmesi

Year 2023, , 117 - 128, 31.10.2023

Souandaou Athoumanı Alı , Fatma Gül Özcan , Omur Karabulut-bulan

https://doi.org/10.59312/ebshealth.1367528

Abstract

Amaç: Safranal, Crocus sativus’un aroma özelliğinden sorumlu bioaktif bir bileşendir. Safranalın antiinflamatuar etkisi dahil olmak üzere farmakolojik özelliklerinin yanı sıra antioksidan aktivitesi birçok çalışmada gösterilmiştir. Çalışmamızda, safranalın serviks karsınoma kökenli C-4 I hücre hattı üzerindeki sitotoksik etkilerini tespit etmek amaçlanmıştır.
Yöntem: Safranalın C-4 I hücre hattı üzerindeki sitotoksik etkisini belirlemek için hücreler belirli sürelerde (2-72 saat) ve konsantrasyonlarda (25-800μM) inkübe edildi. İnkübasyon süresi bitiminde hücrelerin viabilitesi ve safranal antiproliferatif etkisi sırasıyla Mitokondriyal Dehidrojenaz ve Laktat dehidrojenaz enzimlerinin aktiviteleri ile belirlendi. Bununla birlikte, inkübasyon süresince meydana gelen morfolojik değişiklikler Faz kontrast ve ışık mikroskopları aracılığıyla Giemsa boyama kullanılarak gözlemlendi.
Bulgular: elde edilen sonuçlara göre, safranal ile tedavi edilen hücreler kontrol grubu ile karşılaştırıldığında hücrelerin canlılık yüzdesi doza ve inkübasyon süresine bağlı olarak azaldı ve safranal C-4 I hücrelerinin çoğalmasını anlamlı bir şekilde inhibe etti (p<0.05). Nükleer yoğunlaşma, apoptotik ve piknotik hücreler gibi bazı morfolojik değişiklikler ışık mikroskobu altında Giemsa boyama ile incelendi.
Sonuçlar: Elde edilen bulgulara dayanarak safranalın serviks kanseri kökenli C-4 I hücre hatlarına karşı antiproliferatif bir etkiye sahip olduğu söylenebilmektedir.
Anahtar Kelimeler: C-4 I, Safranal, Serviks karsinoma, Sitotoksisite, Antiproliferatif

Keywords

C-4 I, Safranal, Serviks karsinoma, Sitotoksisite, Antiproliferatif

Evaluation of the Antiproliferative Effect of Safranal in C-4 I Cervical Cancer Cell Line

Abstract

Aim: Safranal is a bioactive compound responsible for the aroma propriety of Crocus sativus. Many studies have shown the antioxidant activity of safranal besides some pharmacological properties, including its anti-inflammatory effect. Our study aimed on identifying the cytotoxic effects of safranal on the C-4 I cell line of cervical cancer.
Methods: The cytotoxic effect of safranal on the C-4 I cell line was determined after incubating the cells for certain durations (2 to 72 hours) and concentrations (25 to 800 µM). After incubation, cell viability and anti-proliferation effect of safranal were determined respectively by Mitochondrial Dehydrogenase and Lactate dehydrogenase enzymes activities assays. Additionally, morphological changes occurring during incubation in cells were examined with inverted and optical microscope using Giemsa staining.
Results: According to the results, compared to Control group, the % viability of treated cells was decreased depending on concentration and the incubation time, and safranal significantly inhibited the growth of C-4 I cells (p<0.05). Some morphological changes such as nuclear condensation, and apoptotic and pyknotic cells were examined under light microscopy with Giemsa staining.
Conclusion: In conclusion, based on these results safranal has an antiproliferative effect against cervical cancer C-4 I cell lines.

Keywords

C-4 I, Safranal, Cervical cancer, Cytotoxicity, Antiproliferative

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