Abstract
This research was conducted to determine the effects of green tea (Camellia sinensis L.) Seeds powder as a trout feed supplement on the immune response and disease resistance against Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). For this purpose, the fish were fed with tea (Camellia sinensis L.) seeds powder supplemented diet. Three different fish groups (negative control, positive control and trial group) were formed, and each group was two replicated. Rainbow trout (Oncorhynchus mykiss) fingerling (10 ±0,12g) obtains from research center were placed tanks (10 fish in each tank). Negative control group was established with fish normal diets were fed and not given the bacteria. For the positive control group were used in rainbow trout (Oncorhynchus mykiss) fingerling normal diets were fed and given the bacteria. Trial group was established with rainbow trout (Oncorhynchus mykiss) fingerling were fed with green tea (Camellia sinensis L.) seeds powder supplement diet and given the bacteria. Because of Aeromonas hydrophila symptoms of disease weren’t seen in trout (Oncorhynchus mykiss) fingerlings which were fed with green tea (Camellia sinensis L.) seeds powder supplement diet during ten days. While mortality was not seen innegative control group, was seen in positive control group as 40%. Results from this study confirmed the potential use of green tea (Camellia sinensis l.) seeds as a 10% feed additive for prevention of disease against Aeromonas hydrophila infection in rainbow trout (Oncorhynchus mykiss) fingerling culture
References
- TUİK, Türkiye İstatistik Kurumu, “Su Ürünleri Üretim Miktarı’’, 2014.
- Çelikkale, S, Türkiye Balıkçılığında Sektörel Yapı ve Politikalar, Eğitiminin 10. Yılında Su Ürünleri Sempozyumu, s 13, Ege Üniversitesi Su Ürünleri Fakültesi, 12-14 Kasım, İzmir,1991.
- Tokşen, E, Ege Bölgesinde Yetiştiriciliği Yapılan Çipura ve Levrek Balıklarının Solungaçlarında Görülen Metazoan Parazitler ve Tedavisi, Doktora Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, İzmir, 1999.
- Timur, M. ve Timur, G, Balık Hastalıkları Kitabı, TC. İstanbul Üniversitesi Yayınları, Rektörlük Yayın No: 4426, Su Ürünleri Yayın No: 5, 238, İstanbul, 2003.
- Ateşoğlu, A, Balık Hastalıkları Teşhis Laboratuarının Kuruluşu ve Özellikleri, Veteriner Kontrol ve Araştırma Enstitüsü Müdürlüğü Dergisi, 20-34; 1996.
- Anderson, D.P, In vitro immunization of fish spleen sections and NBT, phagocytic, PFC and antibody assays for monitoring the immune response. (J.S., Stolen, T.C., Fletcher, D.P., Anderson, S.L., Kaattari, and A.F., Rowley, Eds.), Techniques in Fish Immunology, Volume 2, SOS Publications, Fair Haven, N.J. 113-136; 1992.
- FAO, Antibiotics residue in aquaculture products. The state of world fsheries and aquaculture 2002, Rome, Italy, pp. 74-82; 2002.
- Gatlin, D.M.III, Nutrition and fish health. (J E. Halver and R. W. Hardy Eds.) Fish Nutrition, Academic Press, San Diego, CA, USA. 671-702; 2002.
- Sakai, M, Current research status of fish immunostimulants. Aquaculture, 172: 63-92; 1999.
- Sarder, M.R, Thompson, K.D, Penman, D.J, Mcandrew, B.J, Immune responses of Nile tilapia (Oreochromis niloticus L.) clones: I. Non-specific responses. Development and Comparative Immunology, 25: 37-46; 2001.
- Le Breton, A.D, Farming and health management: Prevention and policy measure, Options Meditarraneennes, 86: 207-220; 2009.
- Bricknell, I, Dalmo, R.A, The use of immunostimulants in fish larval aquaculture, Fish and Shellfish Immunology, 19: 457-472; 2005. doi: 10.1016/j.fsi.2005.03.008
- Cho, S.H, Lee, S.M, Park, B.H, Ji, S.C, Lee, J, Bae J. Oh, S.Y, Effect of dietary inclusion of various sources of green tea on growth, body composition and blood chemistry of the juvenile olive flounder, Paralichthys olivaceus, Fish Physiology and Biochemistry, 2006.
- Abdel- Tawwab, M, Ahmad, H.M, Seden, M.E, Sakr, S.F.M, Use of Green Tea, (Camellia sinensis L.), in Practical Diet for Growth and Protection of Nile Tilapia, (Oreochromis niloticus L.), Against Aeromonas hydrophila Infection. Journal of The World Aquaculture Society. Vol.41, No.S2; 2010.
- Suzuki, K, Misaka, N, Sakai, D.K, Efficacy of green tea extract on removal of the ectoparasitic flagellate Ichthyobodo necator from chum salmon, Oncorhynchus keta, and masu salmon, O. masou, Aquaculture Volume 259, Issues 1–4, 8 September Pages 17–27; 2006.
- Cooper, R, Morre, D.J, Morre, D.M, Medicinal benefits of green tea: Part I. Review of noncancer health benefits. Journal of Alternate Complementary Medicine 11, 521-528; 2005.
- Matthews, C.M, Steep your genes in health: Drink tea. Proceedings (Baylor University Medical Centre) 23, 142-144; 2010.
- Xia, E.Q, Deng, G.F, Guo, Y.J, Li, H.B, Biological activities of polyphenols from grapes - Review. International Journal of Molecular Sciences 11, 622-646; 2010.
- Toda, M, Okubo, S, Ohnishi, R. Shimamura, T, Antibacterial and bactericidal activities of Japanese green tea. Japanese Journal of Bacteriology 44, 669-672; 1989.
- Gadang, V.P, Hettiarachchy, N.S, Johnson, M.G, Owens, C.M, Evaluation of antibacterial activity of whey protein isolate coating incorporated with nisin, grape seed extract, malic acid, and EDTA on a turkey frankfurter system. Journal of Food Science 73, 389-394; 2008.
- Chan, E.W.C, Soh, E.Y, Tie, P.P, Law, Y.P, Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. Pharmacognosy Research 3, 266-272; 2011.
- Perumalla, A.V.S, Hettiarachchy, N.S, Green tea and grape seed extracts - Potential applications in food safety and quality. Food Research International 44, 827-839; 2011.
- Çiftci, C, Çay Bitkisi (Camellia sinensis) Tohumunun Balik Sağliği Alaninda Kullaniminin Araştirilmasi, Yüksek Lisans Tezi, Recep Tayyip Erdoğan Üniversitesi Fen Bilimleri Enstitüsü, 2013.
- Miyazaki, T, A histological study of the response to challenge with vibriosis in ayu, Plecoglossus altivelis Temminck and Schlegel, vaccinated by immersion and injection with Vibrio anguillarum bacterin, Journal of Fish Diseases Volume 10, Issue 6, pages 445–452; 1987.
- Korun, J. and Timur, G, Marine Vibrios Associated with Diseased Sea Bass (Dicentrarchus labrax) in Turkey, Journal of FisheriesSciences.com, 2(1): 66-76; 2008.doi:10.3153/jfscom.2008007
- -
- Svobodová, Z, Sudová, E, Nepejchalová, L, Červinka, S, Vykusová, B, Modrá, H. Kolářová, J, Effects of oxytetracycline containing feed on pond ecosystem and health of carp (Cyprinus carpio L.).Acta Vet Brno 75: 571-577; 2006.
Çay Bitkisi (Camellia sinensis) Tohumunun Gökkuşağı Alabalıklarında (Oncorhynchus mykiss) Aeromonas hydrophila Enfeksiyonuna Karşı Kullanımının Araştırılması
Abstract
Bu çalışmada, kültürü yapılan gökkuşağı alabalığının (Oncorhynchus mykiss) fingerling boydaki bireyleri üzerinde Aeromonas hydrophila’nın patojenik etkilerinin ortadan kaldırılması amaçlanmıştır. Bu amaç doğrultusunda alabalık yemine çay bitkisi (Camellia sinensis) tohumu ilave edilmiş ve hastalık etkeni olan Aeromonas hydrophila bakterisi yavrulara enfekte edilerek sonuçları gözlenmiştir.
Çalışmada öncelikle çay tohumu toz haline getirilmiş ve yemlere ilave edilmiştir. Araştırma merkezinden temin edilen fingerling boydaki (10 ±0,12) alabalıklar 10’lu gruplar halinde 6 deneme tankına yerleştirmiştir. Negatif kontrol olarak normal yemle yemlenmiş ve bakteri verilmemiş bir grup oluşturulmuştur. Pozitif kontrol için normal yem ile beslenen ve bakteri enfekte edilen balıklar kullanılmış, deneme için ise katkılı yemlerle beslenen balıklar ve bakteri enfekte edilen balıklar seçilmiştir. 10 günlük yemlemeden sonra yapılan gözlemlerde yeşil çay tohumu verilen gruplarda Aeromonas hydrophila bakterisinin neden olduğu hastalık belirtileri görülmemiştir. Negatif kontrol grubunda ölüm gözlemlenmezken, pozitif kontrol grubunda %40 oranında mortalite gözlemlenmiştir.
Sonuç olarak; elde edilen verilere göre; yeşil çay tohumunun Aeromonas hydrophila bakterisinin meydana getirdiği patojenik etkileri engellediği gözlemlenmiştir
References
- TUİK, Türkiye İstatistik Kurumu, “Su Ürünleri Üretim Miktarı’’, 2014.
- Çelikkale, S, Türkiye Balıkçılığında Sektörel Yapı ve Politikalar, Eğitiminin 10. Yılında Su Ürünleri Sempozyumu, s 13, Ege Üniversitesi Su Ürünleri Fakültesi, 12-14 Kasım, İzmir,1991.
- Tokşen, E, Ege Bölgesinde Yetiştiriciliği Yapılan Çipura ve Levrek Balıklarının Solungaçlarında Görülen Metazoan Parazitler ve Tedavisi, Doktora Tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, İzmir, 1999.
- Timur, M. ve Timur, G, Balık Hastalıkları Kitabı, TC. İstanbul Üniversitesi Yayınları, Rektörlük Yayın No: 4426, Su Ürünleri Yayın No: 5, 238, İstanbul, 2003.
- Ateşoğlu, A, Balık Hastalıkları Teşhis Laboratuarının Kuruluşu ve Özellikleri, Veteriner Kontrol ve Araştırma Enstitüsü Müdürlüğü Dergisi, 20-34; 1996.
- Anderson, D.P, In vitro immunization of fish spleen sections and NBT, phagocytic, PFC and antibody assays for monitoring the immune response. (J.S., Stolen, T.C., Fletcher, D.P., Anderson, S.L., Kaattari, and A.F., Rowley, Eds.), Techniques in Fish Immunology, Volume 2, SOS Publications, Fair Haven, N.J. 113-136; 1992.
- FAO, Antibiotics residue in aquaculture products. The state of world fsheries and aquaculture 2002, Rome, Italy, pp. 74-82; 2002.
- Gatlin, D.M.III, Nutrition and fish health. (J E. Halver and R. W. Hardy Eds.) Fish Nutrition, Academic Press, San Diego, CA, USA. 671-702; 2002.
- Sakai, M, Current research status of fish immunostimulants. Aquaculture, 172: 63-92; 1999.
- Sarder, M.R, Thompson, K.D, Penman, D.J, Mcandrew, B.J, Immune responses of Nile tilapia (Oreochromis niloticus L.) clones: I. Non-specific responses. Development and Comparative Immunology, 25: 37-46; 2001.
- Le Breton, A.D, Farming and health management: Prevention and policy measure, Options Meditarraneennes, 86: 207-220; 2009.
- Bricknell, I, Dalmo, R.A, The use of immunostimulants in fish larval aquaculture, Fish and Shellfish Immunology, 19: 457-472; 2005. doi: 10.1016/j.fsi.2005.03.008
- Cho, S.H, Lee, S.M, Park, B.H, Ji, S.C, Lee, J, Bae J. Oh, S.Y, Effect of dietary inclusion of various sources of green tea on growth, body composition and blood chemistry of the juvenile olive flounder, Paralichthys olivaceus, Fish Physiology and Biochemistry, 2006.
- Abdel- Tawwab, M, Ahmad, H.M, Seden, M.E, Sakr, S.F.M, Use of Green Tea, (Camellia sinensis L.), in Practical Diet for Growth and Protection of Nile Tilapia, (Oreochromis niloticus L.), Against Aeromonas hydrophila Infection. Journal of The World Aquaculture Society. Vol.41, No.S2; 2010.
- Suzuki, K, Misaka, N, Sakai, D.K, Efficacy of green tea extract on removal of the ectoparasitic flagellate Ichthyobodo necator from chum salmon, Oncorhynchus keta, and masu salmon, O. masou, Aquaculture Volume 259, Issues 1–4, 8 September Pages 17–27; 2006.
- Cooper, R, Morre, D.J, Morre, D.M, Medicinal benefits of green tea: Part I. Review of noncancer health benefits. Journal of Alternate Complementary Medicine 11, 521-528; 2005.
- Matthews, C.M, Steep your genes in health: Drink tea. Proceedings (Baylor University Medical Centre) 23, 142-144; 2010.
- Xia, E.Q, Deng, G.F, Guo, Y.J, Li, H.B, Biological activities of polyphenols from grapes - Review. International Journal of Molecular Sciences 11, 622-646; 2010.
- Toda, M, Okubo, S, Ohnishi, R. Shimamura, T, Antibacterial and bactericidal activities of Japanese green tea. Japanese Journal of Bacteriology 44, 669-672; 1989.
- Gadang, V.P, Hettiarachchy, N.S, Johnson, M.G, Owens, C.M, Evaluation of antibacterial activity of whey protein isolate coating incorporated with nisin, grape seed extract, malic acid, and EDTA on a turkey frankfurter system. Journal of Food Science 73, 389-394; 2008.
- Chan, E.W.C, Soh, E.Y, Tie, P.P, Law, Y.P, Antioxidant and antibacterial properties of green, black, and herbal teas of Camellia sinensis. Pharmacognosy Research 3, 266-272; 2011.
- Perumalla, A.V.S, Hettiarachchy, N.S, Green tea and grape seed extracts - Potential applications in food safety and quality. Food Research International 44, 827-839; 2011.
- Çiftci, C, Çay Bitkisi (Camellia sinensis) Tohumunun Balik Sağliği Alaninda Kullaniminin Araştirilmasi, Yüksek Lisans Tezi, Recep Tayyip Erdoğan Üniversitesi Fen Bilimleri Enstitüsü, 2013.
- Miyazaki, T, A histological study of the response to challenge with vibriosis in ayu, Plecoglossus altivelis Temminck and Schlegel, vaccinated by immersion and injection with Vibrio anguillarum bacterin, Journal of Fish Diseases Volume 10, Issue 6, pages 445–452; 1987.
- Korun, J. and Timur, G, Marine Vibrios Associated with Diseased Sea Bass (Dicentrarchus labrax) in Turkey, Journal of FisheriesSciences.com, 2(1): 66-76; 2008.doi:10.3153/jfscom.2008007
- -
- Svobodová, Z, Sudová, E, Nepejchalová, L, Červinka, S, Vykusová, B, Modrá, H. Kolářová, J, Effects of oxytetracycline containing feed on pond ecosystem and health of carp (Cyprinus carpio L.).Acta Vet Brno 75: 571-577; 2006.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Makaleler |
| Authors | |
| Publication Date | August 25, 2015 |
| Submission Date | August 25, 2015 |
| Published in Issue | Year 2015 Volume: 2 Issue: 3 |
