Beta Glucosidase Recognition By Imprinted Polyacrylamide Hydrogels

Year 2019, Volume: 2 Issue: 1, 17 - 23, 20.07.2019

Mervecan Sevildik Ayşe Dinçer , Tülin Aydemir

Abstract

In the present work, selective adsorption of
β-glucosidase using imprinted polyacrylamide hydrogels were studied. For this
purpose imprinted hydrogels were prepared using β-glucosidase as a template
molecule, acrylamide (AAm) as a monomer, N,N’- methylenebisacrylamide (MBAA) as
a crosslinker, ammonium persulphate (APS) and N,N,N’,N’-tetramethylethylene-diamine
(TEMED) as initiators. β-Glucosidase imprinted hydrogel was washed with a
solution of sodium dodecyl sulfate (SDS) and acetic acid to remove the template
molecule. Non-imprinted hydrogel was also prepared without using β-glucosidase
template. The adsorption and recognition performance of hydrogels towards
β-glucosidase was
discussed through adsorption isotherms and adsorption kinetics. In batch
template rebinding experiments, imprinted hydrogels displayed quite high
template binding capacity than non-imprinted hydrogels. The theoretical maximum
adsorption capacity (Qmax) was determined by the Langmuir model, which turned
out to be 7.2 mg/g  and 4.6 mg/g  for imprinted and non-imprinted
hydrogels respectively. A pseudo-second-order model was suitable to interpret
kinetic data.  

Keywords

β-Glucosidase, polyacrylamide, hydrogel

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