The Effects of Various Dental Restorative Materials on Neuroblastoma Cells

Year 2023, Volume: 2 Issue: 2, 14 - 20, 25.10.2023

Pınar Bayram , Mustafa Duzyol , Esra Düzyol , Selina Aksak Karameşe

Abstract

Introduction: Our aim was to investigate the effects of restorative materials such as composite, compomer and glass ionomer, which are frequently used in dentistry, on SH-SY5Y neuroblastoma cells by evaluating oxidative stress parameters, pro- and anti-inflammatory cytokines and apoptosis markers.
Materials and Method: Equa Forte, Dyract AP, Tokuyama Estelite P Quick, Omnichroma, Filtek Z250, SureFil SDR flow restorative materials were used in our study. SH-SY5Y neuroblastoma cells were cultured with restorative materials. Immunohistochemical staining was performed on the experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT levels. One-way ANOVA analysis was used in the statistical evaluation of the obtained results (p<0.05).
Results: In the light of the obtained data, it was observed that the dental filling materials were effective in increasing TGF-beta, IL-10, LPO and CAT levels, and decreasing TNF-alpha, IL-1-beta and IL-6 levels. Histological micrographs also supported the issues. When the H-score levels in the Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups.
Conclusion: Our study shows that biocompatibility cannot be explained by looking at a single cause. Biocompatibility varies with material content, residual monomer amount and solubility. Although all experimental groups have cytotoxic effects, the least effect is seen in the glass ionomer group.

Keywords

Dental filling materials, SHSY5Y, neuroblastoma, inflammation, apoptosis

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