Experimental hypothyroidism increases oxidative stress and apoptosis in ovary of rats

Year 2023, Volume: 2 Issue: 2, 21 - 29, 25.10.2023

Nevra Aydemir Celep , Semin Gedikli , Seçil Nazife Parlak , Elvin Aliyev

Abstract

This study aimed to investigate Hypothyroidism's effects in experimental hypothyroidism-induced female rats. Materials and Methods: To induce Hypothyroidism in rats, 0.05% 6-propyl-2-thiouracil (PTU) was given orally to the Hypothyroid (HT) and L-thyroxine (LT) groups with their drinking water for eight weeks. In the LT group, 0.8 µg/100 g L-thyroxine was provided by subcutaneous for ten days of the experiment. Results: In the histopathological evaluation, Hypothyroidism induced severe dilation in vessels atretic and irregular follicles and damaged the germinal epithelium. These pathologies were decreased in the LT group. In the biochemical analysis, SOD activity, GSH activity, and MDA level were lower in the HT group than Control and LT groups. In the relative protein analysis, Caspase-3, mTOR, P2X7R, NfkB, IL-1β, and TNF-α levels were lower in the Control and LT groups, but the relative protein expression of Caspase-3, mTOR, P2X7R, NfkB, IL-1β, and TNF-α was found as higher in the HT group. The protein expression of Bcl-2 level was higher in the Control and LT groups. Conclusion: The results showed that Hypothyroidism causes ovary tissue injuries —the histopathological and biochemical changes induced by Hypothyroidism in the experimental rat model were ameliorated with L-thyroxine treatment.

Keywords

Hypothyroidism, ovary, oxidative stress, rat

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