Bazı Pamuk Çeşitlerinde iPBS Analiziyle Genetik Varyasyonun Ortaya Çıkarılması

Year 2021, Issue: 21, 67 - 73, 31.01.2021

Ahmet Metin Kumlay , Barış Eren , Serap Demirel , Fatih Demirel , Bünyamin Yıldırım

https://doi.org/10.31590/ejosat.809479

Cited By: 2

Abstract

Bu çalışmada, 10 adet pamuk (Gossypium hirsutum L.) çeşidinin moleküler karakterizasyonu yapılmıştır. DNA temelli 6 adet iPBS markörleri kullanılmış olup, toplamda 36 adet polimorfik bant görülmüş ve ortalama polimorfizm oranı %77.65 olarak saptanmıştır. Markörlerin ortalama polimorfizm değeri (PIC) de 0.27, ortalama gen çeşitliliği (H) değerleri 0.34, ortalama Dice benzerlik katsayı değeri 0.37, korelasyon katsayı değeri (r) de 0.9026 olarak hesaplanmıştır. Genotipler arasındaki genetik ilişki, NTSYS-pc yazılımı kullanılarak belirlenmiş ve 0.0456 ile 0.8387 arasında değiştiği saptanmıştır. İlk üç eigen değerleri toplamı, toplam varyasyonun %71.93’ünü açıklamıştır. Genotiplerin moleküler varyasyon gösterdiği belirlenmiş olup, genetik çeşitlilik sonuçlarının gelecekteki pamuk ıslah çalışmalarının planlanmasında kullanılabileceği sonucuna varılmıştır.

Keywords

Gossypium hirsutum L, Polimorfizm, Islah, iPBS

Supporting Institution

Iğdır Üniversitesi

Project Number

2018-FBE-A05

Reveal of Genetic Variation by iPBS Analysis in Some Cotton Varieties

Abstract

In current study, molecular characterization of 10 cotton (Gossypium hirsutum L.) varieties is performed. There are 6 DNA-based iPBS markers, a total of 36 polymorphic bands were detected and the mean of polymorphism rate was 77.65%. The mean gene diversity (H) of the markers was 0.34 and the mean polymorphism value (PIC) was 0.27. The Dice similarity coefficient value and correlation coefficient was calculated as 0.337 and 0.9026, respectively. Genetic diversity among cotton genotypes using NTSYS-pc software changed 0.0456 and 0.8387. Sum of the first three Eigen value explained 71.93% of the total variation. The cotton genotypes used in this study showed highly molecular variation. It was concluded that results of genetic diversity could be used in the selection planning in future cotton breeding studies.

Keywords

Gossypium hirsutum L., polymorphism, breeding, iPBS

Project Number

2018-FBE-A05

References

• Ashraf, J., Malik, W., Iqbal, M. Z., ALI, K. A., Qayyum, A., Noor, E., ... & Ahmad, M. Q. (2016). Comparative analysis of genetic diversity among bt cotton genotypes using est-ssr, issr and morphological markers. Journal Of Agricultural Science And Technology. 18(2) 517-531.
• FAOSTAT, 2018. http://www.fao.org/faostat/en/#home. (Erişim tarihi: 04/10/2020).
• Fryxell, P.A., (1992). A Revised Taxonomic Interpretation of Gossypium L. (Malvaceae). Rheedea, 2, 108-165.
• Beasley, J. O. (1942). Meiotic chromosome behavior in species, species hybrids, haploids, and induced polyploids of Gossypium. Genetics, 27(1), 25.
• Dice, L.R., (1945). Measures of The Amount of Ecologic Association Between Species. Ecology, 26, 297–302.
• Doyle, J.J., Doyle, J.E., (1990). Isolation of Plant DNA From Fresh Plant Tissue. Focus, 12,13-15.
• Kalendar, R., Antonius, K., Smýkal, P., Schulman, A.H., (2010). IPBS: A Universal Method for DNA Fingerprinting and Retrotransposon Isolation. Theoretical and Applied Genetics, 121(8), 1419-1430.
• Klaedtke, S.M., Caproni, L., Klauck, J., de la Grandville, P., Dutartre, M., Stassart, P.M., Chable, V., Negri, V., Raggi, L., (2017).
• Short-Term Local Adaptation of Historical Common Bean (Phaseolus vulgaris L.) Varieties And İmplications for In Situ Management of Bean Diversity. International Journal of Molecular Sciences, 18(3), 493.
• Liu, K., & Muse, S. V. (2005). PowerMarker: an integrated analysis environment for genetic marker analysis. Bioinformatics, 21(9), 2128-2129.
• Mohammadi, S.A., Prasanna, B.M., (2003). Analysis of Genetic Diversity in Crop Plants Salient Statistical Tools and Considerations. Crop Science, 43(4), 1235-1248.
• Noormohammadi, Z., Hasheminejad-Ahangarani, Y. F., Sheidai, M., Ghasemzadeh-Baraki, S., & Alishah, O. (2013).
• Genetic diversity analysis in Opal cotton hybrids based on SSR, ISSR, and RAPD markers. Genetics and molecular research: GMR, 12(1), 256-269.
• Percival ,A.E., Wendel, J.F., Stewart, J.M., (1999). Taxonomy and germplasm resources. In: Smith CW, Cothren JT, editors. Cotton: Origin, History, Technology, and Production. New York, NY, USA: John Wiley and Sons Inc., pp. 33-63.
• Rohlf, J.F., (2000). NTSYS-pc: Numerical Taxonomy and Multivariate Analysis System. Exeter Software, Setauket, New York.
• Tan, A. (1998). Current status of plant genetic resources conservation in Turkey. In International Symposium on In Situ Conservation of Plant Genetic Diversity, Antalya (Turkey), 4-8 Nov 1996. Central Research Institute for Field Crops.
• Wendel, J. F., Brubaker, C. L., & Percival, A. E. (1992). Genetic diversity in Gossypium hirsutum and the origin of upland cotton. American Journal of Botany, 79(11), 1291-1310.
• Wendel, J.F., Crohn, R.C., (2003). Polyploidy and the evolutionary history of cotton. Advances in Agronomy 78: 139-186.
• Yu, J.Z., Fang, D.D., Kohel, R.J., Ulloa, M., Hinze, L.L., Percy, R.G., Zhang, J., Chee, P., Scheffler, B.E., Jones, D.C., (2012).
• Development of core set of SSR markers for the characterization of Gossypium germplasm. Euphytica. 187(2), 203-213.