Lantanit Metallerle İşaretlenmiş Kolin Klorür Enkapsüle PLGA Nanopartikülleri

Year 2023, Issue: 50, 36 - 46, 30.04.2023

Burcu Uçar

https://doi.org/10.31590/ejosat.1197269

Abstract

Burada, kolin klorür kontrollü salınımını sağlamak için PLGA nanoparçacıklarına yüklenmiştir. Bunların kapsülleme verimleri (EE) ve yükleme kapasiteleri (LC) belirlenmiştir. Ayrıca üretilen nanopartiküller Zetasizer, FT-IR, SEM ve XRD ile karakterize edilmiştir. Nanopartiküllerin yüzeyleri, üç farklı görüntüleme tekniği ile tanı ve tedavi kapasitesini genişletmek için üç farklı doğal lantanit metali Europium, Gadolinium ve Lutetium ile işaretlenerek işlevselleştirilmiştir. Nanopartiküllerin metal işaretlemesi SEM-EDX analizi ile doğrulanmıştır. Kolin klorür yüklü nanoparçacıkların EE’si %62.5-%88.9 arasında değişmiştir. Kolin klorür yüklü nanoparçacıkların LC’si %34.9 ile %61.4 arasında değişmiştir. Serbest nanopartiküllerin Zetasizer analizinden elde edilen ortalama boyut dağılımı 261.0 ± 7.598 nm idi. Kapsüllenmiş nanoparçacıkların Z-ortalama boyutu da 257.5 ila 270 nm arasındaydı. Oldukça negatif zeta potansiyeli, örneğin, serbest NP’ler için -17.85 ± 0.165 mV, nanoparçacıkların yeterince kararlı olduğunu göstermiştir. Metal işaretli NP’lerin element haritalaması, işaretleme prosedürünü doğrulamıştır. Terapötik kolin klorürür yüklü farklı metal işaretli nanoparçacıkların elde edilmesi ile aslında ortak hedefe yönelik ve farklı görüntüleme ve dedeksiyon teknikleri ile limitasyonları ortadan kaldırma beceresine sahip teranostik ajanlar üretilmiştir.

Keywords

Kolin klorür, PLGA, Lantanitler

Choline Chloride Encapsulated PLGA Nanoparticles Labelled with Lanthanide Metals

Abstract

Herein, choline chloride was loaded into PLGA nanoparticles to ensure its controlled release. Encapsulation efficiencies (EE) and loading capacities (LC) of them were determined. Also, the produced nanoparticles were analyzed with Zetasizer, FT-IR, SEM, and XRD. The surfaces of the nanoparticles were functionalized by labeling with three different natural lanthanide metals Europium, Gadolinium, and Lutetium to expand diagnostic and therapeutic capabilities with three different imaging tecniques. Metal labeling of the nanoparticles was confirmed with SEM-EDX analysis. EE of the choline chloride nanoparticles were ranging between 62.5%-88.9%. LC of the choline chloride nanoparticles varied from 34.9 to 61.4. The mean size distribution obtained from the Zetasizer analysis of the free nanoparticles was 261.0 ± 7.598 nm. The Z-average size of the encapsulated nanoparticles also varied from 257.5 to 270 nm. The quite negative zeta potential, for example, -17.85 ± 0.165 mV for free NPs showed that the nanoparticles were sufficiently stable. The elemental mapping of the metal labeled NPs verified the labeling procedure. By obtaining therapeutic choline chloride-loaded nanoparticles with different metal labels, theranostic agents with common target and the ability to eliminate limitations with different imaging and detection techniques have been produced.

Keywords

Choline chloride, PLGA, Lanthanides

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