Detection and identification of citrus long-horned beetle, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae) a new pest in Antalya Province, Turkey by sequencing of mtCOI region

Year 2017, Volume: 41 Issue: 3, 325 - 331, 08.11.2017

Nurdan Topakcı , Utku Yükselbaba , Hüseyin Gocmen

https://doi.org/10.16970/entoted.320617

Cited By: 5

Abstract

In
2016, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae)
was recorded for the first time on Acer negundo L. in Antalya
Province, Turkey. Genetic identification of adult samples was made based on
approximate alignment of a 729-bp sequence of the mitochondrial cytochrome
oxidase I gene region. The sequence alignment in the BLAST analysis from the
NCBI Genbank showed 99% similarity with A. chinensis. The sequence data
was deposited to NCBI Genbank. A phylogenetic analysis was performed by using
the sequence data belonging to A. chinensis and some species of same
genus obtained from the NCBI. According to the phylogenetic analysis,
populations of A. chinensis from Antalya and China were placed in the
same subgroup and therefore it is postulated that the origin of the Antalya
population is China.   

Keywords

Anoplophora chinensis, DNA barcoding, mtCOI, phylogenetic analysis

Antalya ilinde yeni bir zararlı Turunçgil uzun antenli böceği, Anoplophora chinensis (Forster, 1771) (Coleoptera: Cerambycidae)’in tespiti ve mtCOI gen bölgesi sekansına göre tanımlanması

Abstract

Bu çalışma ile Anoplophora
chinensis (Forster, 1771) (Coleoptera: Cerambycidae) Antalya ilinde ilk kez
2016 yılında Acer negundo L. bitkisi üzerinde tespit edilmiştir.
Bununla birlikte, çalışmada A. chinensis’e ait ergin bireylerden DNA
ekstraksiyonu yapılmıştır. Elde edilen DNA örneklerinin mitokondrial sitokrom
oksidaz I gen bölgesinin amplifikasyonu sonucunda yaklaşık 729 bç sekans
dizilimi elde edilmiştir. Elde edilen veriler, NCBI Genbank’ da BLAST analizine
tabi tutulmuş ve A. chinensis ile %99 benzerlik gösterdiği tespit
edilmiştir. Sekans dizilimi NCBI’a kaydedilmiş, Genbank’ta A. chinensis
ve aynı cinse ait bazı türlerin sekans verilerinden yararlanılarak filogenetik
analiz yapılmıştır. Filogenetik analize göre Antalya A. chinensis
popülasyonu Çin popülasyonu ile aynı alt grupta yer almış ve Antalya
popülasyonunun kökeninin Çin olabileceği ağırlık kazanmıştır.

Keywords

Anoplophora chinensis, DNA barkodlama, mtCOI, filogenetik analiz

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