Toxic and in vitro anti-acetylcholinesterase and anti-carboxylesterase effects of various plant extracts on Aphis gossypii Glover, 1877 (Hemiptera: Aphididae)

Year 2019, Volume: 43 Issue: 3, 339 - 346, 01.09.2019

Selçuk Ulusoy , Okan Özgür Yasin Nazım Alpkent

https://doi.org/10.16970/entoted.506727

Cited By: 4

Abstract

The significance of discovering new active substances that are environment friendly when compared to pesticides, agriculturally sustainable, plant-based, and in the status of GRAS (generally regarded as safe) has been increasing every day. For this purpose, leaves of Daphne odora L., Dieffenbachia amoena L., Eucalyptus camaldulensis L., Ficus carica L., Lantana camara L., Matricaria chamomilla L., Mentha pulegium L. and Nerium oleander L. were collected from Adana in 2018. Toxic and in vitro anti-acetylcholinesterase (AChE) and anti-carboxylesterase (CE) activities of aqueous leaf extracts of these species on the important polyphagous pest, Aphis gossypii Glover, 1877 (Hemiptera: Aphididae), were determined after 24 and 72 h. The fastest and greatest toxic effect was obtained with 20% F. carica extract giving 75.6% mortality. This was followed by N. oleander with 71.6% and D. odora with 62.1% mortality. Aphis gossypii in vitro anti-AcHe and anti-CE activities were highest at 10% concentration of the plant extracts and inhibition levels were 51.8-82.5% with F. carica extract, 40.9-54.9% with D. odora extract and 40.2-82.5% with E. camaldulensis extract. In conclusion, D. odora, E. camaldulensis, F. carica and N. oleander extracts gave promising results for future studies on the discovery of potential xenobiotics against A. gossypii and for pest control.

Keywords

Anti-acetylcholinesterase, anti-carboxylesterase, Aphis gossypii, plant extract

Bazı bitki ekstraktlarının Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) üzerine toksik in vitro anti-asetilkolinesteraz ve anti-karboksil esteraz enzim etkisi

Abstract

Pestisitlerle karşılaştırıldığında çevre dostu, tarımsal olarak sürdürülebilir, bitki orjinli ve GRAS statüsünde yeni aktif maddelerin keşfedilmesinin önemi her geçen gün artmaktadır. Bu amaçla, Adana ilinden 2018 yılında Daphne odora L., Dieffenbachia amoena L., Eucalyptus camaldulensis L., Ficus carica L., Lantana camara L., Matricaria chamomilla L., Mentha pulegium L. ve Nerium oleander L. bitkileri toplanmıştır. Bu bitkilere ait yaprakların sulu ekstraktlarının tarımsal alanlarda önemli bir polifag zararlı olan Aphis gossypii Glover, 1877 (Hemiptera: Aphididae) üzerinde toksik, in vitro anti-asetilkolinesteraz (AChE) ve anti-karboksilesteraz (CE) enzim aktiviteleri incelenmiştir. En hızlı ve en yüksek toksik etki %75.6 ölüm oranı ile 3. günde F. carica bitkisinin %20 konsantrasyonunda gözlemlenmiştir. Bunu %71.6 ölüm oranıyla N. oleander, %62.1 ölüm oranıyla D. odora bitkisinde gözlenmiştir. Aphis gossypii in vitro anti-AcHe ve anti-CE aktiviteleri en yüksek test edilen bitki ekstraktlarının %10 konsantrasyonunda görülürken, engelleme seviyeleri sırasıyla F. carica için %51.8-82.5, D. odora için %40.9-54.9, E. camaldulensis için %40.2-82.5 olarak belirlenmiştir. Sonuç olarak, D. odora, E. camaldulensis, F. carica ve N. oleander bitki ekstraktları A. gossypii mücadelesinde ve zararlılarla mücadelede potansiyel ksenobiyotiklerin keşfinde yapılacak sonraki çalışmalar için ümit var sonuçlar ortaya koymaktadır.

Keywords

Anti-asetilkolinestreaz, anti-karboksilesteraz, Aphis gossypii, bitki ekstraktı

References

• Bass, C. & L. M. Field, 2011. Gene amplification and insecticide resistance. Pest Management Science, 67 (8): 886-890.
• Bayhan, S. O., E. Bayhan & M. R. Ulusoy, 2006. Impact of neem and extracts of some plants on development and fecundity of Aphis gossypii Glover (Homoptera: Aphididae). Bulgarian Journal of Agricultural Science, 12: 781-787.
• Birgücü, A. K., S. Satar & İ. Karaca, 2015. Effects of some plant extracts on Aphis gossypii Glover (Hemiptera: Aphididae) and Bemisia tabaci (Gennadius) Takahashi (Hemiptera: Aleyrodidae). Asian Journal of Agriculture and Food Sciences, 3 (2): 149-154.
• Bradford, M. M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytic Biochemistry, 72: 248-254.
• Dadel, E. & S. Saleh, 2017. The impact of terpenoids isolated from seeds of Peganum harmala and leaves Nerium oleander plants against cotton insect Aphis gossypii. International Journal of Chemical Technical Research, 10 (2): 775-777.
• Field, L. M., R. L. Blackman, C. Tyler-Smith & A. L. Devonshire, 1999. Relationship between amount of esterase and gene copy number in insecticide resistant Myzus persicae (Sulzer). Biochemical Journal, 339 (3): 737-742.
• Gershenzon, J. & R. Croteau, 1991. “Herbivores. Their Interactions with Secondary Plant Metabolites: The Chemical Participants, 165-219”. In: Terpenoids (Eds. G. A. Rosenthal & M. R. Berenbaum). Academic Press (Vol. 1), New York, 452 pp.
• Ghada, E. A., E. Manal, E. M. Amal & E. M. Hala, 2017. Application of tomato leaves extract as pesticide against Aphis gossypii Glover (Hemiptera: Aphididae). International Journal of Advanced Research, 5 (4): 286-290.
• Ghosh, S. K., 2015. Integrated field management of aphids (Myzus persicae Sulz. and Aphis gossypii Glov. Together) on potato (Solanum tuberosum L.) using bio-pesticides. International Journal of Science Environment and Technology, 4 (3): 682-689.
• Godfrey, L. D., K. J. Fuson & J. P. Wood, 1997. “Physiological and yield responses of cotton to mid-season cotton aphid infestations in California, 1048-1051”. Proceedings of the Beltwide Cotton Conferences (6-10 January 1997, New Orleans, Louisiana, Tennessee, USA), National Cotton Council of America, Memphis, 1671 pp.
• Gottlieb, O. R., 1990. Phytochemicals: differentiation and function. Phytochemistry, 29 (6): 1715-1724.
• Hansson, D., M. J. Morra, V. Borek & S. D. Eigenbrode, 2012. Green peach aphid [Myzus persicae (Sulzer) (Hemiptera: Aphididae)] control using Brassicaceae ethyl ester oil sprays. Journal of Applied Entomology, 12: 1-10.
• Hemingway, J. & S. H. Karunaratne, 1998. Mosquito carboxylesterases: a review of the molecular biology and biochemistry of a major insecticide resistance mechanism. Medical and Veterinary Entomology, 12 (1): 1-12.
• Henderson, C. F & E. W. Tilton, 1955. Tests with acaricides against brown wheat mite. Journal of Economic Entomology, 48: 157-161.
• Hollingworth, R. M & K. Dong, 2008. “The Biochemical and Molecular Genetic Basis of Resistance to Pesticides in Arthropods, 5-31”. In: Global Pesticide Resistance in Arthropods (Eds. M. E Whalon, D. Mota-Sanchez & R. M. Hollingworth). Wallingford, UK, CABI, 208 pp.
• Houghton, P. J., Y. Ren & M. J. Howes, 2006. Acetylcholinesterase inhibitors from plants and fungi. National Product Reports, 23 (2): 181-199.
• Junqing, Q., Z. Hongyong & L. Qiyuan, 2011. The properties and kinetics of enzymatic reaction in the process of enzymatic extraction of fish oil. Journal of Food Science and Technology, 48 (3): 280-284.
• Korayem, A. M., S. A. Hasabo & H. H. Ameen, 1993. Effects and mode of action of some plant extracts on certain plant parasitic nematodes. Anzeiger für Schädlingskunde, Pflanzenschutz, Umweltschutz, 66 (2): 32-36.
• Nauen, R., 2007. Insecticide resistance in disease vectors of public health importance. Pest Management Science, 63: 628-633.
• Nauen, R., N. Stumpf & A. Elbert, 2002. Toxicological and mechanistic studies on neonicotinoid cross-resistance in Q-type Bemisia tabaci (Hemiptera: Aleyrodidae). Pest Management Science. 58: 868-875.
• Özger, Ş., D. Pohl & İ. Karaca, 2013. The use of neem extracts as bioinsecticide. Turkish Journal of Biological Control, 4 (2): 165-178.
• Ozgur, A. F & E. Sekeroglu, 1986. Population development of Bemisia tabaci (Homoptera: Aleyrodidae) on various cotton cultivars in Cukurova, Turkey. Agriculture Ecosystems and Environment, 17: 83-88.
• Pinto, E. S., E. M. Barros, J. B. Torres & R. C. S. Neves, 2013. The control and protection of cotton plants using natural insecticides against the colonization by Aphis gossypii Glover (Hemiptera: Aphididae). Acta Scientiarum Agronomy, 35 (2): 169-174.
• Raffa, K. F. & T. M. Priester, 1985. Synergists as research tools and control agents in agriculture. Journal of Agricultural Entomology, 2: 27-45.
• Rattan, R. S., 2010. Mechanism of action of insecticidal secondary metabolites of plant origin. Crop Protection, 29 (9): 913-920.
• Scharf, M. B., M. Baumann & D. V. Berkowitz, 2003. The effects of sodium oxybate on clinical symptoms and sleep patterns in patients with fibromyalgia. Journal of Rheumatology, 30: 1070-1074.
• Schmutterer, H., 1988. Potential of azadirachtin containing pesticides for integrated pest control in developing and industrialized countries. Journal of Insect PhysioIogy, 34: 713-719.
• Scott, I. M., H. Jensen, J. G. Scott, M. B. Isman, J. T. Arnason & B. J. R. Philogène, 2003. Botanical insecticides for controlling agricultural pests: piperamides and the colorado potato beetle Leptinotarsa decemlineata Say (Coleoptera: Chrysomelidae). Archives of Insect Biochemistry and Physiology, 54: 212-225.
• Senthil, N. S., C. M., Young, S. H. Yul, P. C. Hoon, K. Kalaivani & K. J. Duk, 2008. Effect of azadirachtin on acetylcholinesterase (AChE) activity and histology of the brown planthopper Nilaparvata lugens (Stål). Ecotoxicology and Environmental Safety, 70 (2): 244-250.
• Shafeek, A., R. P. Jaya Prasanthi, G. H. Reddy, C. S. Chetty & G. R. Reddy, 2004. Alterations in acetylcholinesterase and electrical activity in the nervous system of cockroach exposed to the neem derivative, azadirachtin. Ecotoxicology and Environmental Safety, 59 (2): 205-208.
• Singh, A., Kataria, R., & Kumar, D., 2012. Repellence property of traditional plant leaf extracts against Aphis gossypii Glover and Phenacoccus solenopsis Tinsley. African Journal of Agricultural Research, 7 (11): 1623-1628.
• Singh, D. & A. Singh, 2005. The toxicity of four native Indian plants: Effect on AChE and acid/alkaline phosphatase level in fish Channa marulius. Chemosphere, 60 (1): 135-140.
• Sparks, T. C. & R. Nauen, 2015. IRAC: Mode of action classification and insecticide resistance management. Pesticide Biochemistry and Physiology, 121: 122-128.
• Stumpf, N. & R. Nauen, 2001. Cross-resistance, inheritance and biochemistry of METI-acaricide resistance in Tetranychus urticae (Acari: Tetranychidae). Journal of Economic Entomology, 94: 1577-1583.
• Tomizawa, M. & J. E. Casida, 2005. Neonicotinoid insecticide toxicology: Mechanisms of selective action. Annual Review of Pharmacology and Toxicology, 45: 247-268.
• Völlinger, M., 1987. “The possible development of resistance against neem seed kernel extract and deltamethrin in Plutella xylostella, 543-554”. In: Natural Pesticides from the Neem Tree (Azadirachta indica A. Juss) and Other Tropical Plants, Proceedings of Third International Neem Conference, German Agency for Technical Cooperation (GTZ), (10-15 July 1986, Eschborn, Germany), 703 pp.
• Wink, M. & O. Schimmer, 1999. “Modes of Action of Defensive Secondary Metabolites, 17-133”. In: Functions of Plant Secondary Metabolites and Their Exploitation in Biotechnology (Ed. M. Wink). Annual Plant Reviews No. 3. Sheffield Academic Press, Sheffield, UK, 370 pp.
• Yankova, V., D. Markova, G. Velichkov & N. Velkov, 2014. Plant oils for control of cotton aphid (Aphis gossypii Glov.) in greenhouse cucumbers. Turkish Journal of Agricultural and Natural Sciences, Special Issue: 2 (2014): 1565-1568.
• Zibaee, I., A. R. Bandani, J. J. Sendi, R. Talaei-Hassanlouei & B. Kouchaki, 2010. Effects of Bacillus thurengiensis var. kurstaki, and medicinal plants (Artemisia annua L.) and (Lavandula stoechas L.) extracts on digestive enzymes and Lactate dehydrogenase of Hyphantria cunea Drury (Lepidoptera: Arctiidae). Invertebrate Survival Journal, 7: 251- 261.