Development of methodology for resistance screening of chickpea genotypes collected in Turkey to the root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae)

Year 2020, Volume: 44 Issue: 1, 81 - 89, 01.03.2020

Tohid Behmand , Ece Börteçine Kasapoğlu Uludamar , Jens Berger İbrahim Halil Elekcıoglu

https://doi.org/10.16970/entoted.621304

Cited By: 5

Abstract

The root lesion nematode, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) are considered economically important plant parasitic nematodes affecting chickpea [Cicer arietinum L. (Fabales: Fabaceae)] production. A major strategy to develop resistance to root lesion nematodes in chickpea is to assess and exploit their natural variation. Therefore, nine accessions of wild Cicer reticulatum Ladiz. (Fabales: Fabaceae), Cicer echinospermum P.H.Davis (Fabales: Fabaceae) and domesticate C. arietinum were assessed for resistance to P. thorneiaccording to multiplication rate of the nematode. This study was conducted during 2014-2015 to detect the suitable initial inoculum density (150, 225 and 300) per plant duration of experiment (16 and 20 weeks) for resistance test to P. thornei in chickpea cultivars. There was no significant difference between growing times 16 and 20 weeks and between the initial inoculum density of 225 and 300 nematodes. The only significant difference was observed at a low initial inoculum density of 150 nematodes in all tested cultivars. Therefore, the initial inoculum density of 225 and the growing time of 16 weeks were selected to access of chickpea genotypes for resistance study to P. thornei.

Keywords

Chickpea, optimization, Pratylenchus thornei, resistance, screening method

Supporting Institution

GRDC

Project Number

CSP00185

Thanks

We thank Dr. Abdullah Kahraman (Department of Field Crops, Agriculture Faculty, Harran University) for his help and provision of chickpea seeds.

Türkiye'den toplanan nohut genotiplerinin kök yara nematoduna, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) karşı taranması

Abstract

Kök yara nematodu, Pratylenchus thornei Sher & Allen, 1953 (Tylenchida: Pratylenchidae) dünyada nohut [Cicer arietinum L. (Fabales: Fabaceae)] üretimini etkileyen, ekonomik açıdan önemli bitki paraziti nematod türü olarak kabul edilmektedir. Nohutta kök yara nematodlarına karşı direnç geliştirmenin temel stratejisi, doğal çeşitliliklerini değerlendirmek ve kullanmaktır. Bundan dolayı P. thornei'ye karşı dayanıklılık çalışmalarında, üreme oranı dikkate alınarak, dokuz adet yabani Cicer reticulatum Ladiz. (Fabales: Fabaceae), Cicer echinospermum P.H.Davis(Fabales: Fabaceae) ve yerli C. arietinum türleri değerlendirilmiştir. Bu çalışma, nohut çeşitlerinin P. thornei'ye karşı dayanıklılık denemeleri için, uygun hasat süresi (16 ve 20 hafta) ve bitki başına başlangıç inokulum yoğunluğunu (150, 225 ve 300) belirlemek için 2014-2015 yılları arasında yürütülmüştür. Hasat zamanı 16 ve 20 hafta ile 225 ve 300 başlangıç inokulum yoğunluğu arasında anlamlı bir fark olmadığı, buna karşın bitki başına 150 inokulum yoğunluğunun istatistiki olarak daha düşük etki gösterdiği saptanmıştır. Bu nedenle, nohut çeşitlerinde P. thornei'ye karşı dayanıklılık çalışmaları için en uygun deneme parametrelerinin, 225 başlangıç inokulum yoğunluğu ve 16 hafta hasat zamanı olduğu belirlenmiştir.

Keywords

Nohut, optimizasyon, Pratylenchus thornei, dayanıklılık, tarama yöntemi

Project Number

CSP00185

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