Determining phosphine resistance in rust red flour beetle, Tribolium castaneum (Herbst.) (Coleoptera : Tenebrionidae) populations from Turkey

Year 2015, Volume: 39 Issue: 2, 129 - 136, 10.02.2015

Erhan Koçak , David Schlipalius Ramandeep Kaur Andrew Tuck Paul Ebert Pat Collins Abdullah Yılmaz

https://doi.org/10.16970/ted.17464

Cited By: 20

Abstract

Fumigation with phosphine gas is the primary method of controlling stored grain pests. In Turkey, phosphine has been used extensively since the 1950’s. Even though high levels of phosphine resistance have been detected in several key stored products pests across the world, it has never been studied in Turkey despite this long history of phosphine use. High-level phosphine resistance has been detected and genetically characterised previously in the rust red flour beetle, Tribolium castaneum in other countries. Since this pest is also a common problem in stored grain environment in Turkey, the current study was undertaken for the first time, to investigate the distribution and strength of phosphine resistance in T.castaneum. Four strains of T. castaneum were tested through bioassays for determining the weak and strong phosphine resistance phenotypes on the basis of the response of adults to discriminating phosphine concentrations of 0.03 mg/L and 0.25 mg/L, for 20 hour exposures respectively. Phenotype testing showed all strains exhibited some level of phosphine resistance with a maximum level of 196 fold. Sequencing and genetic testing of seven field-collected strains showed that all of them carried a strong resistance allele in at the rph2 locus similar to the one previously reported. Our results show that strong resistance to phosphine is common in Turkish strains of T. castaneum.

Keywords

Wheat, phosphine resistance, Tribolium castaneum, molecular diagnostic, dihydrolipoamide dehydrogenase, dld

Türkiye Un Biti Tribolium castaneum (Herbst.) popülasyonlarında fosfin direncinin belirlenmesi

Abstract

Depolanmış hububattaki zararlılarla mücadeledeki ana yöntem fosfin gazı ile fumigasyondur. Fosfin gazı Türkiye’de 1950’li yıllardan beri yoğun olarak kullanılmaktadır. Fosfinin uzun yıllardan beri depolanmış hububatta kullanımına ve dünyada da pek çok ülkede yüksek seviyelerde fosfin direncinin belirlenmiş olmasına rağmen Türkiye’de bugüne kadar bu konuda herhangi bir çalışma yapılmamıştır. Yüksek seviyelerde fosfin direnci, un biti Tribolium castaneum’da belirlenerek direnç yapısı genetik olarak tanımlanmıştır. Bu zararlı Türkiye’de depolanmış buğdayda yaygın bir zararlı konumunda olduğundan, bu ilk çalışma fosfin direncinin T. castaneum’daki durumunu ve ülkedeki dağılımını ortaya koymak üzere yürütülmüştür. Dört popülasyon, zayıf ve kuvvetli direnç için sırasıyla ayırıcı fosfin konsantrasyonları olan 0.03mg/L ve 0.25mg/L dozları ile 20 saat süreyle uygulama temeline dayanan bioassaylerle test edilmişlerdir. Bu testlerde popülasyonların tümünde direnç olduğu ve direnç oranının 196 kata kadar ulaştığı belirlenmiştir. Yapılan genetik çalışmalar ve sekans, tüm popülasyonların kuvvetli direnç allellerine sahip olduklarını ve bu popülasyonların daha önce literatürde rapor edilenlere benzer olarak rph2 lokusundaki direnç allelini taşıdıklarını göstermiştir. Çalışma sonuçlarımız, T. castaneum’un Türkiye popülasyonlarında fosfine karşı güçlü bir direncin yaygın olduğunu göstermektedir.

Keywords

Buğday, fosfin direnci, Tribolium castaneum, moleküler tanımlama, dihydrolipoamide dehydrogenase, dld

References

• Abbott, W.S., 1925. A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2): 265–267.
• Anonymous, 2014. (Web page: http://www.agrosanita.com.tr/teknikbilgiler). (Date accessed: June 25, 2014).
• Ahmad, A., M. Ahmed, Nourullah, G.M. Ali, M. Abbas & S. Arif, 2013. Monitoring of resistance against phosphine in stored grain insect pests in Sindh. Middle-East Journal of Scientific Research, 16 (11): 1501-1507.
• Ansell, M.R., 1992. The Mode of Inheritance to Phosphine in Two Species of Stored Products Beetles. Ph.D Dessertation, University of Reading, Reading, UK..
• Attia, F.I. & H.G. Greening, 1981. Survey of resistance to phosphine in Coleopterous pests of grains and stored products in New South Wales. General and Applied Entomology. 4: 98-101.
• Benhalima, H., M.Q. Chaudhry, K. A. Mills & N.R. Price. 2004. Phosphine resistance in stored-product insects collected from various grain storage facilities in Morocco. Journal of Stored Product Research, 40: 241–249.
• Bell, C.H., 2000. Fumigation in the 21st century. Crop Protection. 19: 563–569.
• Champ, B.R. & C.E. Dyte, 1976. Report on the FAO Global Survey of Pesticide Susceptibility of Stored Grain Pests. FAO Plant Protection and Production Services, No. 5, FAO Rome, 297 p.
• Chen, Z., D. Schlipalius, G. Opit, B. Subramanyam & T. W. Phillips, 2015. Diagnostic molecular markers for phosphine resistance in U.S. populations of Tribolium castaneum and Rhyzopertha dominica. PloS ONE (in press, accepted Feb 2015).
• Collins P.J., G.J. Daglish, M. Bengston, T.M. Lambkin & H. Pavic, 2002. Genetics of resistance to phosphine in
• Rhyzopertha dominica (Coleoptera : Bostrichidae). Journal of Economic Entomology, 95(4): 862-869.
• Daglish, G.J. & P.J. Collins, 1999. “Improving the relevance of assays for phosphine resistance, 584–593”. In: Proceedings of the Seventh International Working Conference on Stored-product Protection (Eds.: J. Zuxun, L. Quan, L. Yongsheng, T. Xianchang & G. Lianghua), (14–19 October 1998, Beijing, China). Sichuan Publishing House of Science & Technology, Chengdu, China.
• Daglish G.J., P.J. Collins, H. Pavic & R.A. Kopittke, 2002. Effects of time and concentration on mortality of phosphine-resistant Sitophilus oryzae (L) fumigated with phosphine. Pest Management Science, 58(10): 1015- 1021.
• FAO Method No. 16, 1975. Recommended methods for the detection and measurement of resistance of agricultural pests to pesticides. Tentative method for adults of somemajor pest species of stored cereals, with methyl bromide and phosphine. Plant Protection Bulletin FAO, 23 (1):12–25.
• Heseltine, H. K. & R.H. Thompson, 1957. The use of aluminium phosphide tablets for the fumigation of grain. Milling, 129: 676-783.
• Jagadeesan, R., P.J. Collins, G.J. Daglish, P.R. Ebert & D.I. Schlipalius, 2012. Phosphine resistance in the Rust red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae): Inheritance, gene interactions and fitness costs. PLoS ONE, 7(2): e31582.
• Jagadeesan, R., A. Fotheringham, P.R. Ebert & D.I. Schlipalius, 2013. Rapid genome wide mapping of phosphine resistance loci by a simple regional averaging analysis in the red flour beetle, Tribolium castaneum. BMC Genomics, 14:650.
• Jittanun, C. & W. Chongrattanameteekul, 2014. Phosphine resistance in Thai local strains of Tribolium castaneum (Herbst) and their response to synthetic pheromone. Kasetsart Journal (Natural Science), 48: 9–16.
• Kaur, R., S. Mohankumar, R. Jagadeesan, G. J. Daglish, M. K. Nayak, H. R. Naik, S. Ramasamy, C. Subramanian, P. R. Ebert & D. I. Schlipalius, 2015. Phosphine resistance in India is characterised by a dihydrolipoamide dehydrogenase variant that is otherwise unobserved in eukaryotes. Heredity (in press- accepted Feb 2015)
• Mills, K.A., 1983. Resistance to the fumigant hydrogen phosphide m some stored-product species associated with repeated inadequate treatments. Mitteilungen der Deutschen Gesellschaft fur Allgemeine und Angewandte Entomologie, 4: 98-101.
• Mills, K.A., E.J. Donahaye, S. Navarro & J.G. Leesch, 2001. Phosphine Resistance: Where to Now? 2001. 128 Proc Int Conf on Controlled Atmospheres and Fumigation in Stored Products, Fresno, California.
• Nisa, S. N., I. Bhattacharya, A.G. Tuck, D. Schlipalius & P.R. Ebert, 2011. Mechanisms of Phosphine Toxicity.
• Journal of Toxicology, 1-9, Article ID 494168.
• Opit, G., T. Phillips, M. Aikins, & M. Hasan, 2012. Phosphine resistance in Tribolium castaneum and Rhyzopertha dominica from stored wheat in Oklahoma. Journal of Economic Entomology, 105: 1107-1114.
• Payne, R.W., 2004. GenStat for Windows Release 9. VSN International Oxford, UK; 2004.
• Pimentel, M., L. Faroni, M. Totola & R. Guedes, 2007. Phosphine resistance, respiration rate and fitness consequences in stored-product insects. Pest Management Science, 63: 876-881.
• Rahim, M. & Z. Sulaiman, 1999. Survey of resistance of stored grain pests to phosphine in Malaysia. Proceedings of International Conference on Plant Protection in the Tropics, 5: 226–229.
• Rahim, M., M.E. Faridah & M. Rasali, 2004. Current status of phosphine resistance in stored grain insects in Malaysia. Journal of Tropical Agriculture and Food Science, 32(1): 101–107.
• Rajendran, S., 1999. “Phosphine resistance in stored grain insect pests in India, 635-641”. In: Proceedings of the Seventh International Working Conference on Stored-product Protection (Eds.: J. Zuxun, L. Quan, L. Yongsheng, T. Xianchang & G. Lianghua), (14–19 October 1998, Beijing, China). Sichuan Publishing House of Science & Technology, Chengdu, China.
• Sartori, M.R., I.A. Pacheco & R.M. Vilar, 1990. “Resistance to phosphine in stored grain insects in Brazil, 1041-1050”. In: 5th International Working Conference on Stored Product Protection (Eds.: F. Fleurrat-Lessard & P. Ducom), INRA/SDPV, Bordeaux.
• Schlipalius, D.I., J. Waldron, B.J. Carroll, P.J. Collins & P.R. Ebert, 2001. A DNA fingerprinting procedure for ultra high-throughput genetic analysis of insects. Insect Molecular Biology, 10(6): 579–585.
• Schlipalius D.I., N. Valmas, A.G. Tuck, R. Jagadeesan, L.Ma, R. Kaur, A. Goldinger, C. Anderson, J. Kuang, S. Zuryn, Y.S. Mau, Q. Cheng, P.J. Collins, M.K. Nayak, H.J. Schirra, M.A. Hilliard & P.R. Ebert, 2012. A core metabolic enzyme mediates resistance to phosphine gas. Science, 338 (6108): 807-810.
• Taylor, R.W.D., 1986. “Response of feld strains of some insect pests of stored products, 132-140”. In: Proc. GASGA Seminar on Fumigation Technology in Developing Countries, Tropical Development Research Institute, London.
• Taylor, R.W.D. & D. Halliday, 1986. “The geographical spread of resistance to phosphine by coleopterous pests of stored products, 607–613”. In: Proceedings of the Crop Protection Conference, Pest and Diseases (17–20 November 1986) Brighton, Metropole, England.
• Zeng, L., 1999. “Development and countermeasures of phosphine resistance in stored grain insects in Guangdong, China, 642–647”. In: Proceedings of the Seventh International Working Conference on Stored-product Protection (Eds.: J. Zuxun, L. Quan, L. Yongsheng, T. Xianchang & G. Lianghua), (14–19 October 1998, Beijing, China). Sichuan Publishing House of Science & Technology, Chengdu, China.
• Zettler J.L., W.R. Halliday & F.H. Arthur, 1989. Phosphine resistance in insects infesting stored peanuts in South Eastern United States. Journal of Economic Entomology, 82(6): 1508-1511
• Zettler, J.L. & F.H. Arthur, 2000. Chemical control of stored product insects with fumigants and residual treatments. Crop Protection, 19: 577-582.