Effects of 24-epibrassinolide on root-knot nematode, Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) in tomatoes

Year 2021, Volume: 45 Issue: 1, 13 - 22, 01.03.2021

Çiğdem Gözel

https://doi.org/10.16970/entoted.808416

Cited By: 2

Abstract

In this study conducted in 2020, three concentrations (1, 5 and 10 µM) of 24-epibrassinolide were applied to seedlings of Lycopersicon esculentum Mill. (Solanales: Solanaceae) cv. H2274, which is susceptible to root-knot nematodes, by immersion, spray and irrigation, and its effects against root-knot nematode Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) were investigated. One-thousand second stage juveniles of M. incognita, collected from cucumber roots in a greenhouse located in Çardak, Çanakkale were inoculated on the roots of the plants. After eight weeks, stem fresh weight, stem dry weight, root diameter and longest root length values, in addition to the stem length and stem diameter measured at the beginning and end of the experiment, were recorded. 24-Epibrassinolide, applied by an immersion method, gave similar or better results than the control even in the presence of nematodes. Distilled water plus nematode application showed the highest gall index whereas 5 µM 24-epibrassinolide plus nematode application gave the lowest gall index. The lowest number of egg mass was also obtained from the same concentration of 24-epibrassinolide applied by immersion. As a result, 24-epibrassinolide showed a beneficial effect in terms of reducing the damage caused by the nematodes in tomato plants, depending on the concentration and application method.

Keywords

Biotic stress, brassinosteroids, resistance, root-knot nematode, tomato

Supporting Institution

TÜBİTAK

Project Number

119O660

Thanks

Financial support for this research was provided by TUBİTAK with 119O660 Project number. The author also would like to thank Prof. Dr. Mehmet Mendeş from Çanakkale Onsekiz Mart University, Faculty of Agriculture Department of Animal Science for his valuable help with the statistical analysis.

24-Epibrassinolidin domateslerde kök-ur nematodu, Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae) üzerine etkileri

Abstract

2020 yılında yürütülen bu çalışmada nematoda duyarlı olan Lycopersicon esculentum Mill. (Solanales: Solanaceae) H2274 çeşidinin fidelerine daldırma, spreyleme ve sulama şeklinde 24-epibrassinolidin üç konsantrasyonu (1, 5 ve 10 µM) uygulanmış ve kök-ur nematodu, Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Tylenchida: Meloidogynidae)’a karşı etkileri araştırılmıştır. Meloidogyne incognita’nın Çanakkale, Çardak’ta bir seradan alınan hıyar köklerinden elde edilen ikinci dönem larvaları (J2) 1000 J2/saksı olacak şekilde bitkilerin köklerine verilmiştir. Sekiz hafta sonunda, denemenin başlangıcında ve sonunda ölçülen gövde boyu ve gövde çapına ilave olarak gövde yaş ve kuru ağırlıkları ile kök çapı ve en uzun kök uzunluğu değerleri kaydedilmiştir. Daldırma şeklinde verilen 24-epibrassinolidin nematod varlığında dahi kontrol bitkilerine yakın veya daha iyi sonuçlara neden olduğu belirlenmiştir. Saf su+nematod uygulaması en yüksek gal indeksini, 5 µM 24-epibrassinolid+nematod uygulaması ise en düşük gal indeksini göstermiştir. Aynı konsantrasyondaki 24-epibrassinolid daldırma şeklinde uygulandığında da en az yumurta paketi sayısı tespit edilmiştir. Sonuç olarak, 24-epibrassinolid nematodun zararlarını azaltma yönünden konsantrasyona ve uygulama yöntemine bağlı olarak destekleyici bir etki göstermiş olup nematodların domates bitkileri üzerindeki etkilerini hafifletmiştir.

Keywords

Biyotik stres, brassinosteroidler, dayanıklılık, kök-ur nematodu, domates

Project Number

119O660

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