Isolation and identification of a fungal pathogen, Beauveria bassiana (Bals.-Criv.) Vuill. (Ascomycota: Hypocreales) from the Hatay yellow strain of silkworm, Bombyx mori L., 1758 (Lepidoptera: Bombycidae) in Türkiye

Year 2023, Volume: 47 Issue: 2, 189 - 197, 20.07.2023

Dönüş Gençer , Başak Ulaşlı , Feza Can Cengiz , İsmail Demir

https://doi.org/10.16970/entoted.1218790

Cited By: 1

Abstract

The Hatay yellow strain of silkworm, Bombyx mori L., 1758 (Lepidoptera: Bombycidae) which produces cocoons with an extraordinary yellow color range, is one of the most important endemic and endangered cultural assets in Türkiye. In this study, intense fungal infection and many deaths were detected in the breeding trays in the Hatay yellow breed production facility. An entomopathogenic fungus was isolated from Hatay yellow strain cadavers collected in 2020. According to the morphological and molecular analysis results of the isolate, which was brought into pure culture, it was identified as Beauveria bassiana (Bals.-Criv.) Vuill. (Ascomycota: Hypocreales), the isolate HS1. Phylogenetic analysis results showed that the HS1 strain was very similar (99 %) to the isolates of B. bassiana KJ6 (Iran) and ARSEF 300 (Europe). The concentration-response test using 1 × 104-8 conidia/ml concentrations produced LC50 values of the new strain of 1.2 × 103 and 0.6 × 106 conidia/ml within 7 days against the larvae of Hatay yellow strain and hybrid strain of silkworm, respectively. The results indicated that the virulence of the B. bassiana HS1 strain to the Hatay yellow strain was much more severe and that the Hatay yellow strain had to fight it to survive.

Keywords

Beauveria bassiana, Bombyx mori, entomopathogenic fungi, Hatay yellow strain, silkworm

Supporting Institution

Hatay Mustafa Kemal University, Scientific Research Unit, Hatay Mustafa Kemal University, Türkiye

Project Number

Grant Project No: 21.GAP.001

Thanks

This study was supported by Hatay Mustafa Kemal University, Scientific Research Unit, Hatay Mustafa Kemal University, Türkiye, Grant Project No: 21.GAP.001

Türkiye’den Hatay sarısı ipekböceği ırkı, Bombyx mori L., 1758 (Lepidoptera: Bombycidae)’den bir fungal patojeni olan Beauveria bassiana (Bals.-Criv.) Vuill. (Ascomycota: Hypocreales)’nın izolasyonu ve tanımlanması

Abstract

Olağanüstü sarı renk dağılımına sahip kozalar üreten Hatay sarı ipekböceği ırkı olan Bombyx mori L., 1758 (Lepidoptera: Bombycidae), Türkiye'nin en önemli endemik ve tehlike altındaki kültür varlıklarından biridir. Bu çalışmada, Hatay sarı ırkı üretim tesisinde, yetiştirme tepsilerinde yoğun fungal enfeksiyonu ve birçok ölüm tespit edilmiştir. Entomopatojen bir fungus 2020 yılında toplanan Hatay sarısı ırkı kadavralarından izole edilmiştir. Saf kültür haline getirilen izolat morfolojik ve moleküler analiz sonuçlarına göre Beauveria bassiana (Bals.-Criv.) Vuill. (Ascomycota: Hypocreales) türüne ait HS1 izolatı olarak tanımlanmıştır. Filogenetik analiz sonuçları, HS1 izolatının B. bassiana KJ6 (İran) ve ARSEF 300 (Avrupa) izolatlarına çok benzer (%99) olduğunu göstermiştir. Konsantrasyon-doz testi 1 × 104-8 konidia/ml konsantrasyonları kullanılarak yapılmış ve yeni izolatın 7 günlük LC50 değerleri Hatay sarısı ırkı ve hibrid ırk ipekböceği larvalarına karşı sırasıyla 1.2 × 103 ve 0.6 × 106 konidia/ml olarak belirlenmiştir. Sonuçlar, B. bassiana HS1 izolatının Hatay sarı ırkına verdiği hasarın çok daha şiddetli olduğunu ve Hatay sarı ırkının hayatta kalabilmek için onunla mücadele etmek zorunda kaldığını göstermiştir.

Keywords

Beauveria bassiana, Bombyx mori, entomopatojen fungus, Hatay sarı ırkı, ipekböceği

Project Number

Grant Project No: 21.GAP.001

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