Pathogenicity of some entomopathogenic fungi on wheat weevil Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae)

Year 2023, Volume: 47 Issue: 3 - Year: 2023 Volume: 47 Issue: 3, 259 - 269, 09.10.2023

Mahmut Alper Altınok , Memiş Kesdek , Şaban Kordalı , Ayşe Usanmaz Bozhüyük , Hacer Handan Altınok

https://doi.org/10.16970/entoted.1222114

Abstract

In the present study, the toxicities of seven entomopathogenic fungal isolates, Beauveria bassiana (ARSEF-4984), Isaria farinosa (ARSEF-3580), Isaria fumosorosea (ARSEF-4501), Lecanicillium muscarium (ARSEF-5128), L. muscarium (ARSEF-972), Lecanicillium lecanii (TR38/11) and L. muscarium (Ve6), were tested against the adults of granary weevil, Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae), under laboratory conditions (25±1°C, 75±5% RH and 14h light:10h dark). Studies were conducted in Atatürk University (Erzurum, Türkiye), in 2018. Fungal isolates were sprayed to adults at two different conidial concentrations (1×105 and 1×107 ml-1). Mortality percentages were observed on the 2nd, 4th, 6th, 8th, and 10th days of treatment. A commercial isolate of L. muscarium were used as positive control and sterile water+0.25% Tween 20 used as negative control. The results demonstrated that the mortality rates of S. granarius adults treated with entomopathogenic fungi ranged from 1.01% to 98.9% across 10-day period. Higher concentration and longer exposure periods resulted in increasing virulence on the adult individuals. Among the strains tested, at 1×107 ml-1 concentration, I. fumosorosea, L. muscarium (ARFES-5128) and L. lecanii isolates displayed 97.85%, 94.62% and 93.58% cumulative mortalities respectively, on S. granarius adults by the 10th day of the experiment. These three isolates are regarded as highly promising biological control agents.

Keywords

Entomopathogen, fungi, stored grain

Buğday biti Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) üzerinde bazı entomopatojen fungusların patojenisitesi

Abstract

Bu çalışmada, yedi entomopatojen fungus izolatı Beauveria bassiana, Isaria farinosa (ARSEF-3580), Isaria fumosorosea (ARSEF-4501), Lecanicillium muscarium (ARSEF-5128), L. muscarium (ARSEF-972), Lecanicillium lecanii (TR38/11) ve ticari bir L. muscarium (Ve6), buğday biti, Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) erginlerine karşı laboratuvar şartlarında (25±1°C, 75±5% nisbi nem ve 14sa aydınlık:10sa karanlık) denenmiştir. Çalışma, Atatürk Üniversitesi’nde (Erzurum, Türkiye) 2018 yılında gerçekleştirilmiştir. Fungal izolatlar ergin bireylere iki farklı spor konsantrasyonunda (1x105 ve 1×107 ml-1) spreylenmiştir. Yüzde ölümler uygulamadan sonraki 2., 4., 6., 8. ve 10. günlerde kaydedilmiştir. Pozitif kontrol olarak ticari bir L. muscarium izolatı ve negatif kontrol olarak da steril su+Tween20 (0,25%) kullanılmıştır. Sonuçlar, entomopatojen fungus ile uygulanan S. granarius erginlerinde, uygulamadan sonraki 10 gün süresince ölüm oranlarının %1,01 ile %98,9 arasında değiştiğini göstermiştir. Yüksek konsantrasyon ve daha uzun maruz kalma süreleri ergin bireylerde artan patojenisite ile sonuçlanmıştır. Uygulanan suşlar arasında, 1×107 ml-1 konsantrasyonda I. fumosorosea, L. muscarium (ARFES-5128) ve L. lecanii izolatları, denemenin 10. gününde S. granarius erginlerinde sırasıyla %97,85%, %94,62 ve %93,58 kümülatif ölüm oranlarına ulaşmıştır. Bu üç izolatın biyolojik mücadele kapsamında yüksek derecede ümitvar oldukları değerlendirilmiştir.

Keywords

Entomopatojen, fungus, depolanmış ürün

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