Effects of storage temperature on viability and virulence of entomopathogenic nematodes Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae), Steinernema carpocapsae Weiser, 1955 and Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae)

Year 2023, Volume: 47 Issue: 3 - Year: 2023 Volume: 47 Issue: 3, 247 - 257, 09.10.2023

Alperen Kaan Bütüner , Merve İlktan , Alper Susurluk

https://doi.org/10.16970/entoted.1279074

Cited By: 6

Abstract

Entomopathogenic nematodes (EPN) are a widely used biological control agent. The aim of the study was to detect efficacy and mortalities of some EPN stored at different temperatures and periods. Three EPN species were used in the study. They were Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain, Steinernema carpocapsae Weiser, 1955 TUR-S4 isolate and Steinernema feltiae Weiser, 1955 (Rhabditida: Steinernematidae) TUR-S3 isolate. The species were kept at 4, 15, 25 and 35°C for 7, 14 and 21 days. Subsequently, these species were applied at a dose of 15 Infective juveniles on Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) larvae. The study was carried out in laboratory conditions in 2023. As a result, HBH and TUR-S4 kept at 25°C for 14 days and TUR-S3 for 21 days showed the highest virulence as 93.33%. Mortality rates of the EPN species kept at the specified temperatures were also determined. The results have showed that the highest mortality rates for the HBH, TUR-S4 and TUR-S3 isolates were 11.96% on the 14th day at 35°C, 19.81% on the 21st day at 25°C and 7.39% on the 21st day at 35°C, respectively. This study is an important step in determining suitable temperature conditions for storing and transporting EPN.

Keywords

Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae, storage, temperatures

Supporting Institution

TÜBİTAK

Project Number

219O370

Thanks

This study was financially supported by the TÜBİTAK (The Scientific and Technological Research Council of Türkiye), Project number: 219O370. Moreover, we would like to express our heartfelt thanks to Assoc. Prof. Dr. Hilal SUSURLUK for providing valuable technical support.

Depolama sıcaklığının entomopatojen nematodlar Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae), Steinernema carpocapsae Weiser, 1955 ve Steinernema feltiae Filipjev, 1934 (Rhabditida: Steinernematidae)'nin canlılık ve virülenslikleri üzerine etkileri

Abstract

Entomopatojen nematodlar (EPN) yaygın olarak kullanılan bir biyolojik mücadele ajanıdır. Bu çalışmanın amacı, farklı sıcaklık ve sürelerde depolanan bazı EPN'lerin etkinlik ve ölüm oranlarını belirlemektir. Çalışmada üç EPN türü kullanılmıştır. Bunlar; Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hibrit ırkı, Steinernema carpocapsae Weiser, 1955 TUR-S4 izolatı ve Steinernema feltiae Weiser, 1955 (Rhabditida: Steinernematidae) TUR-S3 izolatıdır. Bu izolatlar 7, 14 ve 21 gün boyunca 4, 15, 25 ve 35°C'de depolandı. Daha sonra bu türler Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) larvaları üzerine 15 Infektif Juvenil dozunda uygulanmıştır. Çalışma, 2023 yılında laboratuvar koşullarında yapılmıştır. Sonuç olarak; HBH ve TUR-S4 25°C'de 14 gün, TUR-S3 ise 21 gün süreyle %93,33 ile en yüksek virülans göstermiştir. Ayrıca, aynı sıcaklıklarda tutulan EPN türlerinde ölüm oranları da belirlenmiştir. Sonuçlar, HBH, TUR-S4 ve TUR-S3 izolatları için en yüksek ölüm oranlarının sırasıyla 35°C'de 14. günde %11,96, 25°C'de 21. günde %19,81 ve 35°C'de 21. günde %7,39 olduğunu göstermiştir. Bu çalışma, EPN'nin depolanması ve nakliyesi sırasında uygun sıcaklık koşullarının belirlenmesinde önemli bir adımdır.

Keywords

Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema feltiae, depolama, sıcaklık

Project Number

219O370

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