Effect of selected pesticides on the orientation of entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae)

Year 2023, Volume: 47 Issue: 3 - Year: 2023 Volume: 47 Issue: 3, 339 - 349, 09.10.2023

Tufan Can Ulu

https://doi.org/10.16970/entoted.1345508

Cited By: 3

Abstract

Entomopathogenic nematodes (EPNs) play a crucial role in biological control, but they can be also applied together with pesticides. Therefore, the compatibility of pesticides with EPNs and the influence on their behavior significantly affect field success. This study investigated how selected pesticides (Deltamethrin, Imidacloprid, Pendimethalin, 2,4-D, and Boscalid + Pyraclostrobin) affect the orientation behavior of three commercial EPN species. Trials were conducted using steel olfactometers followed by the assessment of EPN dispersal ratios after 24 hours. The study was conducted in the Laboratory of the Plant Protection Department, Faculty of Agriculture and Natural Sciences, Bilecik Şeyh Edebali University between 2022 and 2023. According to the results, while nearly all pesticides exhibited a significant impact on the dispersal behavior of EPNs, the most notable effects were observed in the trials involving 2,4-D and Imidacloprid. These two pesticides demonstrated both repellent and attractive effects on different EPN species. The impact of other pesticides was comparatively negligible. All EPN species exhibited higher orientation towards larvae than the control application. The orientation behavior displayed variations depending on the pesticide type and the EPN species involved. It is expected that this study will contribute to our understanding of the relationship between EPNs and pesticides, and ultimately enhancing the efficacy of EPNs.

Keywords

Behavior, Heterorhabditis, pesticide, Steinernema, orientation

Thanks

I would like to thank Osman Sepetçi and Mehmet Çankaya for their support.

Bazı pestisitlerin entomopatojen nematodların (Rhabditida: Heterorhabditidae ve Steinernematidae) yönelimleri üzerine etkisi

Abstract

Entomopatojen nematodlar (EPN’ler) önemli biyolojik mücadele ajanı olmalarına karşın pestisitler ile birlikte de uygulanabilmektedir. Bu nedenle, pestisitlerin EPN’ler ile uyumluluğu ve EPN davranışı üzerindeki etkisi arazi başarısını etkilemektedir. Bu çalışmada, beş pestisitin (Deltamethrin, Imidacloprid, Pendimetalin, 2,4-D ve Boscalid + Pyraclostrobin) üç ticari EPN türünün yönelim davranışını nasıl etkilediği araştırılmıştır. Denemeler, çelik olfaktometrelerde gerçekleştirilmiş ve 24 saatlik inkübasyon sonrasında EPN yayılma oranları belirlenmiştir. Çalışma 2022-2023 yılları arasında Bilecik Şeyh Edebali Üniversitesi Ziraat ve Doğa Bilimleri Fakültesi Bitki Koruma Bölümü Laboratuvarında gerçekleştirilmiştir. Neredeyse tüm pestisitler EPN yayılımı üzerinde önemli etki göstermiş, en farklı sonuçlar 2,4-D ve Imidacloprid denemelerinde tespit edilmiştir. Her iki pestisit de EPN türüne göre hem itici hem de çekici etkide bulunmuştur. Diğer pestisitlerin etkileri daha sınırlı kalmıştır. Tüm EPN türleri, kontrol uygulamasına kıyasla larvalara daha yüksek yönelim göstermiştir. Sonuçlar, EPN türlerine ve pestisitlere bağlı olarak yayılma oranlarında farklılıklar olduğunu göstermiştir. Bu çalışmanın EPN'ler ve pestisitler arasındaki ilişkiyi anlamaya ve EPN uygulama etkinliğini artırmaya katkı sağlayacağı beklenmektedir.

Keywords

Davranış, Heterorhabditis, pestisit, Steinernema, yönelim

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