The role of drought in the efficacy of some entomopathogenic nematodes

Year 2024, Volume: 48 Issue: 1, 103 - 110, 18.03.2024

Alper Susurluk , Alperen Kaan Bütüner

https://doi.org/10.16970/entoted.1425615

Cited By: 3

Abstract

Entomopathogenic nematodes (EPNs) are endoparasitic organisms commonly used in the control of agricultural pests. There are several factors that determine the efficacy of EPNs on hosts, with one of the most significant being soil moisture. The aim of this study is to determine the effectiveness of some EPNs on hosts at different doses and under different soil moisture conditions. The study utilized 1 Hybrid Strain and 3 EPN isolates, Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hybrid strain, Steinernema carpocapsae Weiser, 1955 TUR-S4 isolate, and Steinernema feltiae Weiser, 1955 (Rhabditida: Steinernematidae) TUR-S3 and S-Bilecik isolates. These species were applied to Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) larvae at 5, 10, and 15 Infective Juveniles (IJs) doses, under 1, 4, 7, 10, and 13% soil moisture conditions. The study was conducted in 2024 at Bursa Uludağ University, Faculty of Agriculture, Department of Plant Protection, Nematology Laboratory. As a result, the highest mortality rates on T. molitor larvae were obtained at 13% soil moisture with 15 IJs, 100% for HBH, 93.33% for TUR-S4, 86.67% for TUR-S3, and 83.33% for S-Bilecik. This study carries important implications for understanding the relationship between EPN efficacy on hosts and soil moisture.

Keywords

Heterorhabditis bacteriophora, soil moisture, Steinernema carpocapsae, Steinernema feltiae

Supporting Institution

TUBITAK (The Scientific and Technological Research Council of Türkiye)

Project Number

Grant Project No: 219O370

Thanks

This study was financially supported by by the TUBITAK (The Scientific and Technological Research Council of Türkiye), Project Number: 219O370. We would like to thank Asst. Prof. Dr. Tufan Can Ulu for the S-Bilecik isolate. Also, we would like to extent our heartfelt thanks to Osman Nuri Yaraşir and Eren Ergene for their technical support.

Bazı entomopatojen nematodların etkinliğinde kuraklığın rolü

Abstract

Entomopatojen nematodlar (EPN) tarım zararlıları ile mücadelede yaygın kullanılan endoparazitik organizmalardır. EPN’lerin konukçular üzerinde etkinliğini belirleyen birçok faktör vardır. En önemlilerinden biri ise toprak nemi olarak bilinmektedir. Bu çalışmanın amacı, farklı dozlarda ve farklı toprak nemlerinde EPN’lerin konukçular üzerinde oluşturduğu etkinliği belirlemektir. Çalışmada 1 Hibrit Irk ve 3 EPN izolatı kullanılmıştır. Bunlar, Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) HBH hibrit ırkı, Steinernema carpocapsae Weiser, 1955 TUR-S4 izolatı ve Steinernema feltiae Weiser, 1955 (Rhabditida: Steinernematidae) TUR-S3 ve S-Bilecik izolatlarıdır. Bu türler Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) larvaları üzerine 5, 10 ve 15 Infektif Juvenil (IJ) dozunda, 1, 4, 7, 10 ve 13% toprak neminde uygulanmıştır. Çalışma, 2024 yılında Bursa Uludağ Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Nematoloji Laboratuvarında gerçekleştirilmiştir. Sonuç olarak T. molitor larvaları üzerinde görülen en yüksek ölüm oranları %13 toprak neminde 15 IJ’ de HBH için %100, TUR-S4 için 93.33% TUR-S3 için 86.67% ve S-Bilecik için 83.33% şeklinde elde edilmiştir. Bu çalışma, EPN'nin konukçu üzerindeki etkinliğinin, toprak nemi ile ilişkisinin belirlenmesi açısından önemli sonuçlar taşımaktadır.

Keywords

Heterorhabditis bacteriophora, toprak nemi, Steinernema carpocapsae, Steinernema feltiae

Project Number

Grant Project No: 219O370

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