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Farklı irrigasyon aktivasyon tekniklerinin simüle internal rezorpsiyon kavitelerinde pulpa dokusunu çözme kapasitelerinin karşılaştırılması

Year 2024, Volume: 58 Issue: 2, 64 - 69, 08.05.2024
https://doi.org/10.26650/eor.20241048313

Abstract

Amaç: Bu in vitro çalışmada, simüle internal rezorpsiyon kavitelerinde (İRK) 3 farklı irrigasyon aktivasyon tekniğinin (İAT’ler) organik doku çözme kapasitelerini karşılaştırmak amaçlanmıştır.
Gereç ve Yöntem: Bu çalışma için 90 adet yeni çekilmiş insan maksiller kesici dişi seçildi. Dekoronasyon işleminden sonra kökler bukkolingual yönde uzunlamasına 2 parçaya bölündü. Her bir parçada kök kanalı üzerinde standart bir İRK hazırlandı. Sonra, sığır diş pulpası örnekleri (~ 2,3 mg) tartıldı ve rezorpsiyon kavitelerine yerleştirildi. Kök parçaları yeniden birleştirilerek yapıştırıldı ve dişler rastgele olarak 6 gruba ayrıldı (n=15); sodyum klorur (NaCl) ve sonik irrigasyon (SI), sodyum hipoklorit (NaOCl) ve SI, NaCl ve pasif ultrasonik irrigasyon (PUI), NaOCl ve PUI,NaCl ve lazerle aktifleştirilmiş irrigasyon (LAI), NaOCl ve LAI. Aktivasyon sonrası dişler ayrılarak kavitelerin içindeki doku örnekleri çıkarılıp tartıldı. İlk ve son ölçümler arasındaki fark hesaplandı ve analiz edildi.
Bulgular: İrrigasyon solüsyonunun NaCl olduğu gruplarda SI, PUI ve LAI’dan anlamlı derecede daha başarılı sonuçlara sahipti. İrrigasyon solüsyonunun NaOCl olduğu gruplarda ise, LAI ve PUI arasında anlamlı fark vardı (Grup 6 ˃ Grup 4, p=0.003). NaOCl ile LAI ve SI arasında anlamlı fark yoktu.
Sonuç: Sığır pulpa dokusunun IRK’lerden tamamen çözünmesi, test edilen herhangi bir teknik ile sağlanamadı. Ancak, NaOCl ile LAI, simüle edilmiş İRK’de sığır pulpa dokusunun çözünme kapasitesinde diğer İAT’lerden daha etkiliydi. İlave olarak, NaOCl ile LAI ve SI arasında anlamlı bir fark yoktu.

Project Number

-

References

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  • Lyroudia KM, Dourou VI, Pantelidou OC, Labrianidis T, Pitas IK. Internal root resorption studied by radiography, stereomicroscope, scanning electron microscope and computerized 3D reconstructive method. Dent Traumatol 2002;18:148-52. google scholar
  • Trope M. Root resorption of dental and traumatic origin: classification based on etiology. Pract Periodontics Aesthet Dent 1998;10:515-22. google scholar
  • Patel S, Ricucci D, Durak C, Tay F. Internal root resorption: a review. J Endod 2010;36:1107-21. google scholar
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  • European Society of Endodontology. Quality guidelines for endodontic treatment: consensus report of the European Society of Endodontology. Int Endod J 2006;39:921-30. google scholar
  • Burleson A, Nusstein J, Reader A, Beck M. The in vivo evaluation of hand/rotary/ultrasound instrumentation in necrotic, human mandibular molars. J Endod 2007;33:782-7. google scholar
  • Neelakantan P, Cheng C, Mohanraj R, Sriraman P, Subbarao C, Sharma S. Antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or Er: YAG laser in vitro. Int Endod J 2015;48:602-10. google scholar
  • Conde A, Estevez R, Loroño G, Valencia de Pablo O, Rossi‐Fedele G, Cisneros R. Effect of sonic and ultrasonic activation on organic tissue dissolution from simulated grooves in root canals using sodium hypochlorite and EDTA. Int Endod J 2017;50:976-82. google scholar
  • de Gregorio C, Estevez R, Cisneros R, Heilborn C, Cohenca N. Effect of EDTA, sonic, and ultrasonic activation on the penetration of sodium hypochlorite into simulated lateral canals: an in vitro study. J Endod 2009;35:891-5. google scholar
  • Gulabivala K, Ng Y, Gilbertson M, Eames I. The fluid mechanics of root canal irrigation. Physiol Meas 2010;31:R49-R84. google scholar
  • Klyn SL, Kirkpatrick TC, Rutledge RE. In vitro comparisons of debris removal of the EndoActivator System, the F File, ultrasonic irrigation, and NaOCl irrigation alone after hand-rotary instrumentation in human mandibular molars. J Endod 2010;36:1367-71. google scholar
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  • Lea S, Felver B, Landini G, Walmsley A. Ultrasonic scaler oscillations and tooth-surface defects. J Dent Res 2009;88:229-234. google scholar
  • Blanken J, De Moor RJG, Meire M, Verdaasdonk R. Laser induced explosive vapor and cavitation resulting in effective irrigation of the root canal. Part 1: a visualization study. Lasers Surg Med 2009;41:514-9. google scholar
  • Aldeen RZ, Aljabban O, Milly H, Allouch A, Hamadah O. Effect of Er:YAG laser-activated irrigation on dentine debris removal from different parts of the root canal system: an in vitro study. Dent Med Probl 2018;55:133-8. google scholar
  • Verstraeten J, Jacquet W, De Moor R, Meire M. Hard tissue debris removal from the mesial root canal system of mandibular molars with ultrasonically and laser-activated irrigation: a micro-computed tomography study. Lasers Med Sci 2017;32:1965-70. google scholar
  • Arslan H, Capar ID, Saygili G, Gok T, Akcay M. Effect of photon-initiated photoacoustic streaming on removal of apically placed dentinal debris. Int Endod J 2014;471072-7. google scholar
  • Akcay M, Arslan H, Mese M, Durmus N, Capar ID. Effect of photon-initiated photoacoustic streaming, passive ultrasonic and sonic irrigation techniques on dentinal tubule penetration of irrigation solution: a confocal microscopic study. Clin Oral Investig 2017;21:2205-12. google scholar
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  • Gomes-Filho JE, Aurélio KG, Costa MM, Bernabé PFE. Comparison of the biocompatibility of different root canal irrigants. J Appl Oral Sci 2008;16:137-44. google scholar
  • Giardino L, Ambu E, Becce C, Rimondini L, Morra M. Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic. J Endod 2006;32:1091-3. google scholar
  • Goldberg F, Massone E, Esmoris M, Alfie D. Comparison of different techniques for obturating experimental internal resorptive cavities. Dent Traumatol 2000;16:116-21. google scholar
  • Koskinen KP, Stenvall H, Uitto VJ. Dissolution of bovine pulp tissue by endodontic solutions. Eur J Oral Sci 1980;88:406-11. google scholar
  • de Gregorio C, Arias A, Navarrete N, Del Rio V, Oltra E, Cohenca N. Effect of apical size and taper on volume of irrigant delivered at working length with apical negative pressure at different root curvatures. J Endod 2013;39:119-24. google scholar
  • Mohammadi Z. Sodium hypochlorite in endodontics: an update review. Int Dent J 2008;58:329-41. google scholar
  • Ulusoy Ö, Savur I, Alaçam T, Çelik B. The effectiveness of various irrigation protocols on organic tissue removal from simulated internal resorption defects. Int Endod J 2018;51:1030-6. google scholar
  • De Moor RJ, Meire M, Goharkhay K, Moritz A, Vanobbergen J. Efficacy of ultrasonic versus laser-activated irrigation to remove artificially placed dentin debris plugs. J Endod 2010;36:1580-3. google scholar
  • De Groot S, Verhaagen B, Versluis M, Wu MK, Wesselink P, Van Der Sluis L. Laser‐activated irrigation within root canals: cleaning efficacy and flow visualization. Int Endod J 2009;42:1077-83. google scholar
  • DiVito E, Peters OA, Olivi G. Effectiveness of the erbium: YAG laser and new design radial and stripped tips in removing the smear layer after root canal instrumentation. Lasers Med Sci 2012;27:273-80. google scholar
  • Deleu E, Meire MA, De Moor RJ. Efficacy of laser-based irrigant activation methods in removing debris from simulated root canal irregularities. Lasers Med Sci 2015;30:831-5. google scholar
  • Lloyd A, Navarrete G, Marchesan MA, Clement D. Removal of calcium hydroxide from Weine Type II systems using photon-induced photoacoustic streaming, passive ultrasonic, and needle irrigation: a microcomputed tomography study. J Appl Oral Sci 2016;24:543-8. google scholar
  • Iandolo A, Amato M, Abdellatif D, Barbosa AFA, Pantaleo G, Blasi A, Franco V, Silva EJNL. Effect of different final irrigation protocols on pulp tissue dissolution from an isthmus model. Aust Endod J 2021;47:538-43. google scholar

Pulp tissue dissolution capacities of different irrigation agitation techniques in artificial internal resorption cavities

Year 2024, Volume: 58 Issue: 2, 64 - 69, 08.05.2024
https://doi.org/10.26650/eor.20241048313

Abstract

Purpose: The aim of this in vitro study was to compare the organic tissue dissolution capacities of 3 different irrigation agitation techniques (IATs) in artificial internal root resorption cavities (IRCs).
Materials and Methods: Ninety freshly extracted maxillary human incisors were selected. After decoronation procedure, the roots were split longitudinally, and a standard IRC were prepared in the canals on each half of the roots. Then, the bovine pulp samples (~ 2,3 mg) were previously weighed and placed into the cavities. The root fragments were reassembled and cemented to create a circular IRC within the canal. Teeth samples were randomly divided into 6 groups (n = 15); sodium chlorur (NaCl) and sonic irrigation (SI), sodium hypochlorite (NaOCl) and SI, NaCl and passive ultrasonic irrigation (PUI), NaOCl and PUI, NaCl and laser activated irrigation (LAI), NaOCl and LAI. After that, the teeth were decemented and the tissue samples inside the cavities were weighed again. The percentage of weight loss was calculated and statistically analyzed.
Results: SI has significantly more successful results than PUI and LAI in groups which the irrigant was NaCl. There was also a significant difference between LAI and PUI in groups which the irrigant was NaOCl (Group 6 ˃ Group 4, p = .003). There was no significant difference between LAI and SI with NaOCl.
Conclusion: Complete dissolution of bovine pulp tissue from IRCs was not achieved by any tested techniques. However, the LAI with NaOCl was more effective than other IATs. In addition, there is no significant difference between the LAI and SI with NaOCl.

Supporting Institution

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Project Number

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Thanks

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References

  • Patel S, Ford TP. Is the resorption external or internal? Dent Update 2007;34:218-29. google scholar
  • Lyroudia KM, Dourou VI, Pantelidou OC, Labrianidis T, Pitas IK. Internal root resorption studied by radiography, stereomicroscope, scanning electron microscope and computerized 3D reconstructive method. Dent Traumatol 2002;18:148-52. google scholar
  • Trope M. Root resorption of dental and traumatic origin: classification based on etiology. Pract Periodontics Aesthet Dent 1998;10:515-22. google scholar
  • Patel S, Ricucci D, Durak C, Tay F. Internal root resorption: a review. J Endod 2010;36:1107-21. google scholar
  • Haapasalo M, Endal U. Internal inflammatory root resorption: the unknown resorption of the tooth. Endod Topics 2006;14:60-79. google scholar
  • Tronstad L. Root resorption-etiology, terminology, and clinical manifestations. Dent Traumatol 1988;4:241-52. google scholar
  • European Society of Endodontology. Quality guidelines for endodontic treatment: consensus report of the European Society of Endodontology. Int Endod J 2006;39:921-30. google scholar
  • Burleson A, Nusstein J, Reader A, Beck M. The in vivo evaluation of hand/rotary/ultrasound instrumentation in necrotic, human mandibular molars. J Endod 2007;33:782-7. google scholar
  • Neelakantan P, Cheng C, Mohanraj R, Sriraman P, Subbarao C, Sharma S. Antibiofilm activity of three irrigation protocols activated by ultrasonic, diode laser or Er: YAG laser in vitro. Int Endod J 2015;48:602-10. google scholar
  • Conde A, Estevez R, Loroño G, Valencia de Pablo O, Rossi‐Fedele G, Cisneros R. Effect of sonic and ultrasonic activation on organic tissue dissolution from simulated grooves in root canals using sodium hypochlorite and EDTA. Int Endod J 2017;50:976-82. google scholar
  • de Gregorio C, Estevez R, Cisneros R, Heilborn C, Cohenca N. Effect of EDTA, sonic, and ultrasonic activation on the penetration of sodium hypochlorite into simulated lateral canals: an in vitro study. J Endod 2009;35:891-5. google scholar
  • Gulabivala K, Ng Y, Gilbertson M, Eames I. The fluid mechanics of root canal irrigation. Physiol Meas 2010;31:R49-R84. google scholar
  • Klyn SL, Kirkpatrick TC, Rutledge RE. In vitro comparisons of debris removal of the EndoActivator System, the F File, ultrasonic irrigation, and NaOCl irrigation alone after hand-rotary instrumentation in human mandibular molars. J Endod 2010;36:1367-71. google scholar
  • Van der Sluis L, Versluis M, Wu M, Wesselink P. Passive ultrasonic irrigation of the root canal: a review of the literature. Int Endod J 2007;40:415-426. google scholar
  • Lea S, Felver B, Landini G, Walmsley A. Ultrasonic scaler oscillations and tooth-surface defects. J Dent Res 2009;88:229-234. google scholar
  • Blanken J, De Moor RJG, Meire M, Verdaasdonk R. Laser induced explosive vapor and cavitation resulting in effective irrigation of the root canal. Part 1: a visualization study. Lasers Surg Med 2009;41:514-9. google scholar
  • Aldeen RZ, Aljabban O, Milly H, Allouch A, Hamadah O. Effect of Er:YAG laser-activated irrigation on dentine debris removal from different parts of the root canal system: an in vitro study. Dent Med Probl 2018;55:133-8. google scholar
  • Verstraeten J, Jacquet W, De Moor R, Meire M. Hard tissue debris removal from the mesial root canal system of mandibular molars with ultrasonically and laser-activated irrigation: a micro-computed tomography study. Lasers Med Sci 2017;32:1965-70. google scholar
  • Arslan H, Capar ID, Saygili G, Gok T, Akcay M. Effect of photon-initiated photoacoustic streaming on removal of apically placed dentinal debris. Int Endod J 2014;471072-7. google scholar
  • Akcay M, Arslan H, Mese M, Durmus N, Capar ID. Effect of photon-initiated photoacoustic streaming, passive ultrasonic and sonic irrigation techniques on dentinal tubule penetration of irrigation solution: a confocal microscopic study. Clin Oral Investig 2017;21:2205-12. google scholar
  • Kuştarcı A, Er K. Efficacy of laser activated irrigation on apically extruded debris with different preparation systems. Photomed Laser Surg 2015;33:384-9. google scholar
  • Gomes-Filho JE, Aurélio KG, Costa MM, Bernabé PFE. Comparison of the biocompatibility of different root canal irrigants. J Appl Oral Sci 2008;16:137-44. google scholar
  • Giardino L, Ambu E, Becce C, Rimondini L, Morra M. Surface tension comparison of four common root canal irrigants and two new irrigants containing antibiotic. J Endod 2006;32:1091-3. google scholar
  • Goldberg F, Massone E, Esmoris M, Alfie D. Comparison of different techniques for obturating experimental internal resorptive cavities. Dent Traumatol 2000;16:116-21. google scholar
  • Koskinen KP, Stenvall H, Uitto VJ. Dissolution of bovine pulp tissue by endodontic solutions. Eur J Oral Sci 1980;88:406-11. google scholar
  • de Gregorio C, Arias A, Navarrete N, Del Rio V, Oltra E, Cohenca N. Effect of apical size and taper on volume of irrigant delivered at working length with apical negative pressure at different root curvatures. J Endod 2013;39:119-24. google scholar
  • Mohammadi Z. Sodium hypochlorite in endodontics: an update review. Int Dent J 2008;58:329-41. google scholar
  • Ulusoy Ö, Savur I, Alaçam T, Çelik B. The effectiveness of various irrigation protocols on organic tissue removal from simulated internal resorption defects. Int Endod J 2018;51:1030-6. google scholar
  • De Moor RJ, Meire M, Goharkhay K, Moritz A, Vanobbergen J. Efficacy of ultrasonic versus laser-activated irrigation to remove artificially placed dentin debris plugs. J Endod 2010;36:1580-3. google scholar
  • De Groot S, Verhaagen B, Versluis M, Wu MK, Wesselink P, Van Der Sluis L. Laser‐activated irrigation within root canals: cleaning efficacy and flow visualization. Int Endod J 2009;42:1077-83. google scholar
  • DiVito E, Peters OA, Olivi G. Effectiveness of the erbium: YAG laser and new design radial and stripped tips in removing the smear layer after root canal instrumentation. Lasers Med Sci 2012;27:273-80. google scholar
  • Deleu E, Meire MA, De Moor RJ. Efficacy of laser-based irrigant activation methods in removing debris from simulated root canal irregularities. Lasers Med Sci 2015;30:831-5. google scholar
  • Lloyd A, Navarrete G, Marchesan MA, Clement D. Removal of calcium hydroxide from Weine Type II systems using photon-induced photoacoustic streaming, passive ultrasonic, and needle irrigation: a microcomputed tomography study. J Appl Oral Sci 2016;24:543-8. google scholar
  • Iandolo A, Amato M, Abdellatif D, Barbosa AFA, Pantaleo G, Blasi A, Franco V, Silva EJNL. Effect of different final irrigation protocols on pulp tissue dissolution from an isthmus model. Aust Endod J 2021;47:538-43. google scholar
There are 34 citations in total.

Details

Primary Language English
Subjects Dentistry (Other)
Journal Section Original Research Articles
Authors

Simay Koç 0000-0002-9446-5655

Kürşat Er 0000-0002-0667-4909

Gülçin Haciguliyeva 0000-0002-7893-4044

Ziya Osmanlı 0000-0001-6084-0843

Lala Cabbarova 0000-0003-2114-4305

Hüseyin Karayılmaz 0000-0003-2075-6350

Project Number -
Publication Date May 8, 2024
Submission Date December 27, 2021
Published in Issue Year 2024 Volume: 58 Issue: 2

Cite

EndNote Koç S, Er K, Haciguliyeva G, Osmanlı Z, Cabbarova L, Karayılmaz H (May 1, 2024) Pulp tissue dissolution capacities of different irrigation agitation techniques in artificial internal resorption cavities. European Oral Research 58 2 64–69.