Bruselloz Tanısı ve Epidemiyolojik Çalışmalarda Moleküler Yöntemlerin Kullanılması

Year 2019, , 68 - 72, 25.03.2019

Oktay Genç

https://doi.org/10.32707/ercivet.537995

Cited By: 3

Abstract

Brusellozis, Latin Amerika, Orta doğu, Afrika ve Asya’da endemik seyreden zoonotik bir enfeksiyondur. Günümü-ze kadar 11 Brusella türü tanımlanmıştır. Brusella türleri içerisinde insanlarda en şiddetli enfeksiyona neden olan B. melitensis türü olup bunu B. suis ve B. abortus izlemektedir. Brusella türleri farklı sayılarda biyovarlardan oluşmakta ve immun serumlar ile aglütinasyon, bazı boyalar ve fajlara duyarlık özelliklerine göre de ayırımları yapılmaktadır. Enfeksi-yon kaynağının tespiti, enfeksiyon ve/veya aşı suşlarının karakterizasyonu ve yeni türlerin ortaya konulması önceleri biyovarlara göre yapılır iken, son yıllarda Loop Mediated Isothermal Amplification (LAMP) ve Restriction Fragment Length Polymorphism (RFLP) gibi PZR tabanlı yöntemler ile Multilokus variable number tandem repeat analysis (MLVA), Multilokus Sekans Tiplendirme (MLST) gibi moleküler test ve yöntemler ile yapılmaktadır. Bu derleme ile bu yöntemler ve bu alanda yapılan çalışmalar ile ilgili güncel bilgiler sunulmaktadır.

Keywords

Brusellozis, LAMP, moleküler tanı, MLST, MLVA

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