Türkiye’de Yetiştirilen Kıl ve Halep Keçilerinde Beta-Laktoglobulin (β-LG)/ SacII Polimorfizminin Belirlenmesi

Year 2017, Volume: 14 Issue: 2, 107 - 112, 15.08.2017

Korhan Arslan , Bilal Akyüz , Esma Gamze İlgar Fadime Özdemir Mehmet Ulaş Çınar

Abstract

Bu çalışmada Türkiye yerli keçi ırklarından Kıl ve Halep (Damascus) ırklarında beta-laktoglobulin (β-LG) gen polimorfizminin belirlenmesi amaçlanmıştır. Çalışmada toplam 269 baş keçide β-LG genotipleri SacII enzim kesimi ile belirlenmiştir. Örneklere ait PCR ürünlerinin SacII enzimi ile kesimleri sonucunda A ve B olarak isimlendirilen iki allel belirlenmiştir. İncelenen ırklarda AA genotipinin frekansı Kıl keçilerinde 0.721, Halep keçilerinde ise 0.948 olarak belir-lenmiştir. AB genotipinin frekansı ise Kıl keçilerinde 0.279, Halep keçilerinde ise 0.052 olarak belirlenmiştir. İncelenen her iki keçi ırkında da BB genotipine rastlanılmamıştır. Çalışma sonunda Kıl keçilerinde Hardy-Weinberg (H-W) dengesinden sapma gözlenmişken (P<0.05), Halep ırkının H-W dengesinde olduğu belirlenmiştir. Bu çalışma ile Halep keçilerinde β-LG-SacII polimorfizmi ilk kez belirlenmiştir.

Keywords

Beta-laktoglobulin geni, keçi, polimorfizm, RFLP

Detection of Beta-Laktoglobulin (β-LG)/ SacII Gene Polymorphism in Hair and Damascus Goat Breeds in Turkey

Abstract

This disquisition was aim to detect the allele structures of beta-lactoglobulin (β-LG) gene in Hair (172) and Halep (Damascus) (97) breeds that have been grown in Turkey. A total of 269 goats were analized for the β-LG-SacII polymorphism by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). In the exam-ined breeds, digestion the PCR products with SacII restriction enzyme show two alleles (A and B) and two genotypes (AA and AB). The genotype BB was not located on both of Hair and Halep variety. Genotypic frequencies of allele B were 0.721 and 0.948 for AA and 0.279 and 0.052 for AB in order. Deflection from Hardy-Weinberg equilibrium was tracked in the Hair goat breed (P<0.05) while deflection from Hardy-Weinberg equilibrium was not tracked in the Halep goats. Hereby, this work provided data on the β-LG polymorphism of two Turkish goat variety. Moreover, this work reported the presence of a genetic β-LG- SacII polymorphism in Halep goat variety in Turkey for the first time.

Keywords

Beta-lactoglobulin gene, goat, polymorphism, RFLP

References

• 1. Ağaoğlu ÖK, Kul BÇ, Akyüz B, Elmaz Ö, Metin MÖ, Saatci M, Ertuğrul O. Identifica-tion of β-lactoglobulin gene SacII polymorp-hism in Honamli, Hair and Saanen goat breeds reared in Burdur vicinity. Kafkas Univ Vet Fak Derg 2012; 18 (3): 385-8. 2. Ahmed S, Othman OE. Detection of goat β-lactoglobulin genotypes using restriction fragment length polymorphism. J of Genetic Eng Biotechnol 2005; 3(1): 31-41. 3. Amigo L, Recio I, Ramos M. Genetic poly-morphism of ovine milk proteins: Its influ-ence on technological properties of milk-a review. Int Dairy J 2000(1); 10: 135-49. 4. Pedrosa S, Uzun M, Arranz JJ, Gutiérrez-Gil B, San Primitivo F, Bayón Y. Evidence of three maternal lineages in Near Eastern sheep supporting multiple domestication events. Proc Biol Sci 2005; 272(1577): 2211-7. 5. Bonfatti V, Di Martino G, Cecchinato A, De-gano L, Carnier P. Effects of beta-kappa-casein (CSN2-CSN3) haplotypes, beta-lactoglobulin (BLG) genotypes, and detailed protein composition on coagulation proper-ties of individual milk of Simmental cows. J Dairy Sci 2010; 93(1): 3809-17. 6. El-Hanafy AA, El-Saadani MA, Eissa M, Maharem GM, Khalifa ZA. Polymorphism of β-lactoglobulin gene in Barki and Damascus and their cross bred goats in relation to milk yield. Biotechnol Anim Husb 2010; 26(1-2): 1-12. 7. El Hanafy AAM, Qureshi MI, Sabir J, Mutawakil M, Ahmed MMM, El Ashmaoui H, Ramadan HAMI, Abou-Alsoud M, Sadek MA. Nucleotide sequencing and DNA poly-morphism studies of beta-lactoglobulin gene in native Saudi goat breeds in relation to milk yield. Czech J Anim Sci 2015; 60(3): 132-8. 8. Elmacı C, Oner Y, Koyuncu M. Allelic frequ-ency of a SacII RFLP at exon 7 of the β-lactoglobulin gene in Turkish Hair goat breed. AJAVA 2009; 4(1):130-3. 9. Elmacı C, Öner Y, Koyuncu M. Saanen keçilerinde β-laktoglobulin genotiplerinin PCR-RFLP yöntemi ile belirlenmesi. Hay-vansal Üretim Dergisi 2008; 4(1): 1-4. 10. Ertuğrul O, Akyüz B. Halk elinde yetiştirilen Ankara keçilerinde (Capra hircus) bazı kan protein polimorfizmi. Ankara Üniv Vet Fak Derg 2000; 47(1): 23-9. 11. Food and Agriculture Organization (FAO). http://faostat3.fao.org/download/Q/QA/E, Erişim tarihi: 15.05.2015. 12. Guney O, Torun O, Ozuyanık O, Darcan N. Milk production, reproductive and growth performances of Damascus goats under northern Cyprus conditions. Small Rum Res 2006; 65(1-2): 176-9. 13. Gürcan N. Çeşitli Tiftik ve Kıl keçisi popü-lasyonlarında β-laktoglobulin polimorfizmi. Yüksek lisans tezi, Ankara Ü Fen Bilimleri Enstitüsü, Ankara, 2005. 14. Hayes HC, Petit EJ. Mapping of theβ-lactoglobulin gene and of immunoglobulin M heavy chain-like sequence to homolo-gous cattle, sheep and goat chromosomes. Mamm Genome 1993; 4(4): 207-10. 15. Kaçar C, Zonturlu AK, Karapehlivan M, Arı UÇ, Öğün M, Çitil M. The effects of L-carnitine administration on energy metabo-lism in pregnant Halep (Damascus) goats. Turk J Vet Anim Sci 2010; 34(2): 163-71. 16. Kahilo Kh., EL-Shazly S, El-Khadrawy A, Fattouh I. Genetic polymorphism in β-lactoglobulin gene of some goat breeds in Egypt and its influence on milk yield. Life Science Journal 2014;11(10): 232-8. 17. Kumar A, Rout PK, Roy R. Polymorphism of β-lacto globulin gene in Indian goats and its effect on milk yield. J Appl Genet 2006; 47(1): 49-53. 18. Matějíček A, Matějíčková J, Němcová E, Jandurová OM, Štípková M, Bouška J, Fre-lich J. Joint effects of CSN3 and LGB geno-types and their relation to breeding values of milk production parameters in Czech Fleckvieh. Czech J Anim Sci 2007; 52(4): 83-7. 19. Patel RK, Chauhan JB, Singh KM, Soni KJ. Allelic frequency of kappa-casein and beta-lactoglobulin in Indian crossbred (Bos tau-rus × Bos indicus) dairy bulls. Turk J Vet Anim Sci 2007; 31(6): 399-402. 20. Pedrosa S, Uzun M, Arranz JJ, Gil BG, Primitova FS, Bayon Gil BG, Primitova FS, Bayon Y. Evidence of three maternal line-ages in near eastern sheep supporting mul-tiple domestication events. Proc Biol Sci 2005; 272: 2211-17. 21. Pena RN, Sanchez A, Folch JM. Character-ization of genetic polymorphism in goat β-lactoglobulin gene. J Dairy Res 2000; 67(2): 217-24. 22. Rachagani S, Gupta ID, Gupta N, Gupta SC. Genotyping of β-lactoglobulin gene by PCR-RFLP in Sahiwal and Tharparkar cat-tle breeds. BMC Genet 2006; 7(1): 31. 23. Şengonca M, Koşum N. Koyun ve Keçi Ye-tiştirme (Keçi Yetiştirme ve Islahı). Ege Üni-versitesi Basımevi, Bornova-İzmir 2005;11-5. 24. Tsiaras AM, Bargouli GG, Banos G, Boscos CM. Effect of kappa-casein and beta-lactoglobulin loci on milk production traits and reproductive performance of Holstein cows. J Dairy Sci 2005; 88(1): 327-34. 25. Türkiye İstatistik Kurumu (TUİK). Hayvancı-lık istatistikleri. Erişim Adresi: http://tuikapp.tuik.gov.tr/hayvancilikapp/hayvancilik.zul, Erişim tarihi: 15.05.2015. 26. Türkyılmaz O. Yüksek süt verimli saanen keçilerinde süt protein polimorfizmi, Doktora tezi, Uludağ Ü. Sağlık Bilimleri Enstitüsü, Bursa, 2003;21 27. Yüksel M, Akyüz B. Kayseri ve civarında halk elinde yetiştirilen Kıl keçilerinde βeta-laktoglobulin gen polimorfizminin PCR-RFLP yöntemi ile belirlenmesi. Sağlık Bilim-leri Dergisi 2014; 23(2): 62-6.