Retrospective Investigation of C. dubliniensis Prevalence Among Stock C. albicans Strains Isolated From Healthy Individuals And Patients With Candida-Associated Prosthetic Stomatitis By Molecular Methods

Year 2021, , 64 - 69, 30.12.2021

Derya Kazancı , Tanju Kadir Korkut Ulucan

https://doi.org/10.29228/erd.10

Abstract

Objectives: Candida-related denture stomatitis (C-RDS), is the most frequent form of oral candidiasis, being detected in approximately 50%-65% of denture-wearing patients. Candida albicans has been shown to be the principal species responsible for inflammatory pathology. Candida dubliniensis, first described in Dublin in 1995, was found to be very similar to C. albicans in terms of phenotypic features and the type of lesion it causes. It was necessary to determine the historical prevalence of C.dubliniensis due to the possibility that the strains identified as C.albicans in the past years were C.dubliniensis.
Materials and Methods: In this study, 81 yeast strains isolated from oral samples and identified as C.albicans only by photypic methods and kept in the stock collection until 2003 were re-examined by molecular methods.
Results: In the genotypic examination performed by polymerase chain reaction and restriction endonuclease reactions, 4 (8.8%) of 45 C.albicans strains isolated from healthy individuals were found to be C.dubliniensis, but all 36 strains isolated from patients with C-RDS were C.albicans.
Conclusions: As a result of this retrospective study, it was found that the prevalence of C.dubliniensis among 81 yeast strains isolated orally was 4.9%, but it was not effective in the pathogenesis of C-RDS.

Keywords

C.dubliniensis, C.albicans, Candida related denture stomatitis

Supporting Institution

Marmara University Scientific Research Projects Coordination Unit

Sağlıklı bireylerden ve Candida- ilişkili protez stomatitili hastalardan izole edilmiş stok C.albicans suşları arasındaki C.dubliniensis prevalansının moleküler yöntemlerle retrospektif olarak araştırılması

Abstract

Amaç: Candida-ilişkili protez stomatiti (C-İPS), protez takan hastaların yaklaşık %50-65'inde saptanan oral kandidiyazın en sık görülen şeklidir. Candida albicans’ın enflamatuar patolojiden sorumlu başlıca tür olduğu gösterilmiştir. İlk kez 1995 yılında Dublin'de tanımlanan Candida dubliniensis’in fenotipik özellikler ve neden olduğu lezyon türü açısıdan C. albicans’a çok benzediği saptanmıştır. Geçmiş yıllarda C.albicans olarak tanımlanan suşların C.dubliniensis olma olasılığı nedeniyle C.dubliniensis’in tarihsel prevalansını belirlemeye gerek duyulmuştur.
Gereç ve Yöntem: Bu çalışmada, oral örneklerden izole edilip sadece fotipik yöntemlerle C.albicans olarak tanımlanan ve 2003 yılına kadar stok koleksiyonunda tutulan 81 maya suşu moleküler yöntemlerle yeniden incelenmiştir.
Bulgu: Polimeraz zincir reaksiyonu ve restriksiyon endonükleaz reaksiyonları ile yapılan genotipik incelemede sağlıklı bireylerden izole edilen 45 C.albicans suşun 4’ü (%8,8) C.dubliniensis, ancak CİPS’li hastalardan izole edilen 36 suşun tamamının C.albicans olduğu tespit edilmiştir.
Sonuç: Bu retrospektif araştırmanın sonucunda ağızdan izole edilmiş 81 maya suşu arasında C.dubliniensis prevelansının % 4.9 olduğu, ancak C-İPS patogenezinde etkin olmadığı anlaşılmıştır.

Keywords

C.dubliniensis, C.albicans, Candida-ilişkili protez stomatiti

References

• 1.Aboualigalehdari E, Birgani MT, Fatahinia M, Hosseinzadeh M. Oral colonization by Candida species and associated factors in HIV-infected patients in Ahvaz, southwest Iran. Epidemiol Health. 2020; 42: e2020033.
• 2.Acosta LD, Perez-Camacho O, Perez-Camacho O, Acosta R, Escobar DM, Gallardo CA, Sanchez-Vargas LO. (2020). Reduction of Candida albicans biofilm formation by coating polymethyl methacrylate denture bases with a photopolymerized film. The Journal of Prosthetic Dentistry. 2020; 124(5): 605-613.
• 3.Akpan A, Morgan R. Oral Candidiasis. Postgrad Med J. 2002; 78: 455-459.
• 4.Arias CM, Vicenta JL, Sahand IH, Eguia A, De-Juan A, Madariaga L, Aguirre JM, Eraso E, Qindos G. Isolation of Candida dubliniensis in denture stomatitis. Archives of Oral Biology. 2008; 54: 127-131.
• 5.Aslani N, Janbabaei G, Abastabar M, Meisi JF, Babaeian M, Khodavaisy S, Boekhout T, Badali H. Identification of uncommon oral yeasts from cancer patients by MALDI-TOF mass spectrometry. BMC Infectious Diseases. 2018; 18(24).
• 6. Badaro MM, Bueno FL, Arnez RM, Oliveira VC, Macedo APM, Souza RF, Paranhos HFO, Silva-Lovato CH. The effects of three disinfection protocols on Candida spp., denture stomatitis, and biofilm: A parallel group randomized controlled trial. The Journal of Prosthetic Dentistry. 2020; 124(1): 690-698.
• 7. Baran I, Nalçaci R. Self-reported denture hygiene habits and oral tissue conditions of complete denture wearers. Arch. Gerontol. Geriatr. 2009; 49: 237–241.
• 8. Clark-Ordo´ nez I, Callejas-Negrete OA, Arechiga-Carvajal ET, Mourino-P ˜ erez ´ RR. Candida species diversity and antifungal susceptibility patterns in oral samples of HIV/AIDS patients in Baja California, Mexico. 2017; Medical Mycology, 55: 285-294.
• 9. Coleman, DC, DJ Sullivan, DE Bennett, GP Moran, HJ Barry, Shanley DB. Candidiasis: the emergence of a novel species, Candida dubliniensis. 1997; AIDS, 11: 557–567.
• 10. Coronado-Castello, Jimenez-Soriano Y. Clinical and microbiological dianosis of candidiasis. J Clin Exp Dent. 2013; 5(5): 279-286.
• 11. Deo PN, Deshmukh R. Oral Microbiome: Unveiling the fundamentals. J Oral Maxillofac Pathol. 2019; 23(1): 122-128.
• 12. Gad MM, Fouda SM. Current perspectives and future of Candida albicans-associated denture stomatitis treatment. Dent Med Probl.2020; 57(1): 95-102.
• 13. Gasparoto TH, Dionı´sio TJ, Oliveira CE, Porto VC, Gelani V, Santos CF, Campanelli AP, Lara VS. Isolation of Candida dubliniensis from denture wearers. Journal of Medical Microbiology.2009; 58: 959-962.
• 14. Gauch LMR, Pedrosa SS, Silveira-Gomes F, Renata AE, Marques-de-Silva S. Isolation of Candida spp. From denture-related stomatitis in Para, Brazil. Brazilian Journal of Microbiology. 2018; 49: 148-151.
• 15. Gendreau L, Loewy ZG. Epidemiology and etiology of denture stomatitis. J Prosthodont. 2011; 20(4):251–260.
• 16. Gleiznys A, Zdanavičienė E, Juozas Žilinskas. Candida albicans importance to denture wearers. A literature review. Stomatologija, Baltic Dental and Maxillofacial Journal. 2015; 17: 54-66.
• 17. Hellstein JW, Marekg C. Candidiasis: Red and white manifestations in the oral cavity. Head Neck Pathol. 2019; 13: 25–32.
• 18. Hilmioğlu S, Aydemir Ş, İnci R. Gürel SÖ, Tümbay E. Türkiye’de ilk kez izole edilen Candida dubliniensis kökeni. İnfeksiyon Derg. 1998; 12(4): 545-8.
• 19. Khan İ, Ahmad T, Manzoor N, Rizvi MA, Uqba Raza U, Premchandani S. Evaluating the role of local host factors in the candidal colonization of oral cavity: A review update. NJMS. 2020; 11 (2): 169-175.
• 20. Kimsa Ł, Tokarska-Rodak M. Occurrence of Candida spp. In healthy oral microbiota. Health Probl Civilizat. 2020;14:124-30.
• 21. Kurnatowska AJ. Search for correlation between symptoms and sings of changes in the oral mucosa and presence of fungi. Mycoses. 2001; 44:379-382.
• 22. Liverio HS, Ruiz LS, Nishikaku A, Souza AC, Paula CR, Domaneschi C. Phenotypic and genotypic detection of Candida albicans and Candida dubliniensis strains isolated from oral mucosa of AIDS pediatric patients. Rev Inst Med Trop S Paulo.2017; 59: e14.
• 23. Lourenço AG, Ribeiro AERA , Nakao C , Motta ACF, Antonio LGL , Machado AA , Komesu MC. Oral Candida spp carriage and periodontal diseases in HIVinfected patients in Ribeirão Preto, Brazil. Rev Inst Med Trop São Paulo. 2017; 59: e29.
• 24. Maenza RJ, Merz WG, Gorbach SL, Bartlett NR, Blacklow NR. Candida albicans and Related Species. In: Infectious Diseases. 1998; 2th Ed, p. 213-221.
• 25. Marcos-Arias C, Vicente JL, Sahand IH, Eguia A, De-Juan A, Madariaga L, Aguirre JM, Eraso E, Quindós G. Isolation of Candida dubliniensis in denture stomatitis. Arch Oral Biol. 2009; 54 (2): 127- 131.
• 26. Martin M, Henriques M, Ribeiro AP, Fernandes R, Gonçalves V, Seabra A, Azeredo J, Oliveira R. Oral Candida carriage of patients attending a dental clinic in Braga, Portagul. Rev Iberoam Micol. 2010; 27(3): 119-124.
• 27. Mendez-Serrano J, Velazquez-Enriquez U, Contreras-Bulnes R, Rosa-Gómez I, Sawada T, Yamaguchi R. Adhesion of Candida albicans AND Streptococcus mutans to silver nanoparticle-modified polymetylmetharcrylate. Interciencia. 2020; 45(1).
• 28. Millsop JW , Faze LN. Oral candidiasis. Clin. Dermatol. 2016; 34: 487–494.
• 29. Moran GP, Sanglard D, Donnelly SM, Shanley DB, Sullivan DJ, Coleman DC. Identification and expression of multidrug transporters responsible for fluconazole resistance in Candida dubliniensis. Antimicrobial Agent and Chemotherapy. 1998. 1819-1830.
• 30. Mosca CO, Moragunes MD, Brena S, Rosa AC, Ponton J. Isolation of Candida dubliniensis in a teenager with denture stomatitis. Med Oral Pathol Oral Cir Bucal. 2005; 10: 28–35.
• 31. Mousa MA, Lynch E, Kielbassa AM. Denture-related stomatitis in new complete denture wearers and its association with Candida species colonization: a prospective case-series. Quintessence International (Berlin, Germany : 1985). 2020; 51(7):554-565.
• 32. Muadcheingka T, Tantivitayakul P. Distribution of Candida albicans and non-albicans Candida species in oral candidiasis patients: Correlation between cell surface hydrophobicity and biofilm forming activities. Science Direct Archives of Oral Biology. 2015; 60: 894-901.
• 33. Mun M, Yap T, Alnuaimi AD, Adams GG, McCullough MJ. Oral candidal carriage in asymptomatic patients. Aust Dent J. 2016; 61: 190–195.
• 34. Noble, SM, Gianetti, BA ve Witchley, JN. Candida albicans cell type switching and functional plasticity in the mammalian host. Nat Rev Microbiol.2017; 15, 96–108.
• 35. Odds FC, Nuffel LV, Dams G. Prevalence of Candida dubliniensis ısolates in a yeast stock collectin. Journal of Clinical Microbiology. 1998; 36(10): 2869-2873.
• 36. Quindós G, Carrillo-Muñoz AJ, Arévalo MP, Salgado J, Alonso-Vargas R, Rodrigo JM, Ruesga MT, Valverde A, Pemán J, Cantón E, Martín-Mazuelos E, Pontón J. Candida dubliniensis to current and new antigungal agents. Chemotherapy. 2000; 46: 395-401.
• 37. Peltroche H, Dohmen H, Haase G. Recovery of Candida dubliniensis from sputum of cystic fibrosis patients. Mycosis. 2002; 45: 15-18.
• 37. Pinjon, E., D. Sullivan, I. Salkin, D. Shanley, and D. Coleman. Simple, inexpensive, reliable method for differentiation of Candida dubliniensis from Candida albicans. J Clin Microbiol. 1998; 36: 2093–2095.
• 38. Pinto E, Riberio IC, Ferreira NJ, Fortes CE, Fonseca PA, Figuerial MH. Correlation between enzyme production, germ tube formation, and susceptibility to fluconazole in Candida species isolated from patients with denture -related stomatitis and control individuals. J Oral Pathol Med. 2008; 37 (10): 587-592.
• 39. Prakash B, Shekar M, Maiti B, Karunasagar I, Padiyath S. Prevalence of Candida spp. Among healty denture and nondenture wearers with respect to hygiene and age. The Journal of Indian Prosthodontic Society. 2015; 15(1): 29-32.
• 40. Raju SB, Rajappa S. Isolation and identification of Candida from the oral cavity. International Scholarly Research Network ISRN Dentistry. 2011; ID 487921.
• 41. Rodrigues CF, Rodrigues ME, Henriques M. Candida sp. İnfections in patients with diabetes mellitus (Review). J Clin Med. 2019; 8 (76).
• 42. Romeo O, Racco C, Criseo G. Amplification of hyphal wall protein 1 gene to distinguish Candida albicans from Candida dubliniensis. Journal of Clinical Microbiology. 2006; 44: 2590-2592.
• 43. Samaranayake L. Commensal oral Candida in Asian cohorts. Int J Oral Sci. 2009; 1:2-5.
• 44. Sampath A, Weerasekera M, Dilhari A, Gunasekara C, Bulugahapitiya U. Type 2 diabetes mellitus and .oral Candida colonization: Analysis of risk factors in a Sri Lankan cohort. Acta Odontologica Scandinavica. 2019; 77(7).
• 45. Sant’Ana Mariano PL, Milan EP, Matta DA, Colombo AL. Candida dubliniensis Identification in Brazilian Yeast Stock Collection. Mem Inst Oswaldo Cruz, Rio de Janeiro. 2003; 98(4): 533-538.
• 46. Salerno C, Pascale M, Contaldo M, Espozito V, Busciolano M, Milillo L, Guida A, Petruzzi M, Serpico R. Candida-associated stomatitis (Review). Med Oral Patol Cir Bucal. 2011; 16(2): 139-143.
• 47. Senna AM , Vieirab MMF, Machado-de-Senac RM , Bertolind AO, Núñeze SC , Ribeirof MS. Photodynamic inactivation of Candida ssp. on denture stomatitis. A clinical trial involving palatal mucosa and prosthesis disinfection. Photodiagnosis and Photodynamic Therapy. 2018; 22: 212-16.
• 48. Sharma A. Oral candidiasis: An oppırtunistic infection: A review. International Journal of Applied Dental Sciences. 2019; 5(1): 23-27.
• 49. Singh A, Verma R, Murari A, Agrawal A. Oral candidiasis: An overview. J. Oral Maxillofac Pathol. 2014; 18:81–85.
• 50. Sullivan, DJ, Westerneng TJ, Haynes KA, Bennett DE, Coleman DC. Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals. Microbiology. 1995; 141:1507–1521.
• 51. Thiyahuddin NM, Lamping E, Rich AM, Cannon RD. Yeast species in the oral cavity of older people: A comparison between people living in their own homes and those in rest homes. Journal of Fungi. 2019; 5(30).
• 52. Willis AM, Coulter WA, Sullivan DJ, Coleman DG, Hayes JR, Bell PM ve arkadaşları. Isolation of Candida dubliniensis from insulin-using diabetes mellitus patients. J Oral Pathol Med. 2000; 29: 86– 90.
• 53.Vila T, Sultan AS, Montelongo-Jauregui D, Jabra-Rizk MA. Oral candidiasis: A disease of opportunity.Journal of Fungi. 2020; 6(15).
• 54.Yücel A, Kantarcıoğlu AS. Candida albicans kökenlerinin kaç tanesi Candida dubliniensis? 1. Ulusal Mantar Hastalıkları ve Klinik Mikoloji Kongresi Kitabı.1996; 4-6 Mayıs, İzmir, Türkiye, s. 263-264.
• 55. Zomorodian K, Haghighi NN, Rajee N, Pakshir K, Tarazooie B, Vojdani M, Sedaghat F, Vosoghi M. Assessment of Candida species colonization and denture-related stomatitis in complete denture wearers. Medical Mycology. 2011; 49: 208-211.