An in-vitro study on the effectiveness of different irrigation solutions against Enterococcus faecalis with/without Erbium laser activation.

Year 2020, Volume: 4 Issue: 2, 45 - 51, 31.12.2020

Gülşah Balan , Sadık Kalaycı Ayşen Yarat , Serap Akyüz Fikrettin Şahin

https://doi.org/10.35333/ERD.2020.265

Abstract

Objective: Questions have been raised on the safety of prolonged use of NaOCl. There is a demand on developing alternative agents and activation methods for effective irrigation in a short time. Our study compares sodium hypochlorite to different irrigants with and without laser activation to evaluate antibacterial efficiency against Enterococcus faecalis.

Methods: Bacteria was inoculated into 54 human teeth and incubated for 28 days to build up biofilm. The teeth then were randomly divided according to the irrigation method into two; (1) syringe (S) and (2) Erbium laser activation (LA) groups. In each group, teeth were divided into 4 experimental subgroups to evaluate the antibacterial properties of four different solutions of saline (NaCl), sodium hypochlorite (NaOCl), sodium pentaborate pentahydrate (NaB) and deuterium depleted water (DDW). For S subgroups the amount of applied irrigant solution was 10 mL and for LA subgroups it was 10 mL. Samples were taken from canal wall and colony forming units were determined by plate counting after 24 hours. The survival rates of the bacteria were calculated as data.

Results: Both techniques were reduced the bacterial survivors and no statistically difference was found between S and LA (p>0.05). As expected, NaOCl-LA found more effective than Saline-LA (p<0.05) but not superior than NaOCl-S (p>0.05). NaB solution showed similar antibacterial effect as NaOCl (p>0.05)

Conclusion: The most effective NaOCl solution was followed by NaB, DDW and Saline, respectively. Laser activation did not increase effectiveness of solutions compared to syringe irrigation.

Keywords

deuterium depleted water, Enterococcus faecalis, Erbium laser activation, sodyum pentaborat pentahidrat, sodium hypochlorite

Supporting Institution

Marmara University Scientific Research Projects Unit-BAPKO

Project Number

SAG-C-DRP-241018-0573

Thanks

This work is supported in part by the grant Marmara University Scientific Research Projects Unit. I would like to thank all co-authors and the staff of Yeditepe University Biocidal and AR-GE Laboratory unit.

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