Effect of Indigo Naturalis on the Viability of Human Periodontal Ligament Fibroblast Cells: An In Vitro Study

Year 2024, Volume: 8 Issue: 2, 49 - 54, 29.08.2024

Aslı Aktan , Sema Tuğçe Aydın , Ayşegül Tiryaki , Dilruba Baykara , Canan Doğan , Oğuzhan Gündüz , Turgut Taşkın , Ömer Birkan Ağralı

https://doi.org/10.29228/erd.70

Abstract

Objective: In this in vitro research, it was aimed to examine the viability levels of human periodontal ligament fibroblast (hPDLF) cells by applying different concentrations of Indigo naturalis (IN).

Materials and Methods: Within the scope of our study, IN was dissolved in DMSO and three different concentrations were prepared:, 5 µg/ml, 10 µg/ml and 15 µg/ml. In order to evaluate cell viability, cells were seeded in 96-well plates, repeated 4 times for each concentration. Indigo Naturalis was applied to the cells for 24 hours. 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) experimental protocol was followed for viability assessment. Statistical analyzes of the data were evaluated using the SPSS package program. Statistical significance was accepted as p<0.05.

Results: While it was observed that there was no significant change in the test groups with IN application compared to the control group, a significant difference was detected between 15 μg/ml and 5 μg/ml concentration.

Conclusion: The findings obtained within the limits of this study reveal that IN has similar effects at different concentrations on the viability of hPDLF cells. There is a need for studies in which IN is investigated by preparing it in different concentrations and monitoring it for longer periods of time.

Keywords

Indigo naturalis, cell viability, periodontal ligament fibroblast

İndigo Naturalis’in İnsan Periodontal Ligament Fibroblast Hücrelerinin Canlılığı Üzerine Etkisi: Bir İn Vitro Çalışma

Abstract

Amaç: Bu in vitro araştırmada, insan periodontal ligament fibroblast (İPDLF) hücrelerine farklı konsantrasyonlarda İndigo naturalis (İN) uygulanarak hücrelerin canlılık düzeylerinin araştırılması hedeflenmiştir.

Gereç ve Yöntemler: Çalışmamız dahilinde İndigo naturalis DMSO ile çözülerek 5 µg/ml, 10 µg/ml ve 15 µg/ml olacak şekilde üç farklı konsantrasyon hazırlandı. Hücre canlılığını değerlendirmek amacıyla, 96 kuyucuklu plakalara her konsantrasyon için 4 defa tekrarlanacak şekilde hücrelerin ekimi gerçekleştirildi. Hücrelere 24 saat süreyle İndigo Naturalis uygulandı. Canlılık değerlendirmesi için 3-(4,5-dimetiltiyazol-2-il)-2-5-difeniltetrazolyum bromür (MTT) deney protokolü izlendi. Verilerin istatistiksel analizleri SPSS paket programı kullanılarak değerlendirildi. İstatistiksel anlamlılık p<0.05 olarak kabul edildi.

Bulgular: İN uygulamasının kontrol grubuna kıyasla test gruplarında anlamlı değişiklik olmadığı görülürken 15 μg/ml ve 5 μg/ml konsantrasyon arasında anlamlı fark tespit edilmiştir.

Sonuç: Bu çalışmanın sınırları içerisinde sağlanan bulgular, İN’nin iPDLF hücrelerinin canlılığı üzerine farklı konsantrasyonlarda benzer etki gösterdiğini ortaya koymaktadır. İN’nin daha farklı konsantrasyonlarda hazırlanarak ve daha uzun süre aralıklarında takip edilerek araştırıldığı çalışmalara gereksinim vardır.

Keywords

İndigo naturalis, periodontal ligament fibroblastı, hücre canlılığı

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