HPLC Analysis of Phenolic Compounds from Gypsophila aucheri Boiss. and Investigation of Antioxidant and Cytotoxic Activity of Gypsophila aucheri Boiss. exracts

Year 2018, Volume: 11 Issue: 2, 168 - 181, 31.08.2018

Ahmet Altay

https://doi.org/10.18185/erzifbed.411194

Abstract

Bu çalışma, Gypsophila aucheri Boiss. ekstrelerinin
antioksidan ve antiproliferatif aktivitelerinin yanı sıra RP-HPLC tekniği ile
fenolik içeriğini araştırmak için tasarlanmıştır. Ekstrelerin antioksidan
aktiviteleri 1,1-difenil-2-picrilhidrazil (DPPH) ve 2,2′-azinobis-
(3-etilbenzotiazolin-6-sülfonik asit) (ABTS) radikallerini giderme, Bakır(II)
iyonlarını indirgeme (KUPRAK) ve Fe²⁺ iyonlarını
şelatlama kapasiteleri üzerinden değerlendirildi. Antiproliferatif aktivite
için, ekstrelerin MCF-7 (insan meme kanseri), HT-29 (kolon kanseri) ve HepG2
(karaciğer kanseri) hücrelerine karşı test edildi. Antioksidan aktivite
sonuçları, Gypsophila aucheri metanol ekstresinin DPPH ve ABTS testlerine
göre su ekstraktından daha güçlü antioksidan aktivite gösterdiğini, su
ekstresinin ise KUPRAK ve metal şelatlama aktivitesi testlerinde metanol
ekstresinden daha üstün olduğu gözlendi. Sitotoksisite testleri sonucunda,
metanol ekstresinin su ekstresine kıyasla daha düşük IC50 değerleriyle test
edilen tüm kanser hücre hatlarına karşı daha güçlü antiproliferatif aktivite
sergilediğini gösterdi. Bununla birlikte, her iki ekstrenin de pozitif
kontrollere kıyasla orta düzeyde biyolojik aktivite sergilediği saptandı. Elde
edilen veriler, Gypsophila aucheri Boiss.’in yüksek fitokimyasal içeriğiyle
birlikte çarpıcı antioksidan ve orta antiproliferatif potansiyelleri
nedeniyle bu bitki kaynağının gıda ve nutrasötik endüstri için umut verici
bir kaynak olarak değerlendirilebileceğini göstermektedir.

Keywords

Gypsophila aucheri, phenolic compounds, antioxidant activity, antiproliferative activity

HPLC Analysis of Phenolic Compounds from Gypsophila aucheri Boiss. and Investigation of Antioxidant and Cytotoxic Activity of Gypsophila aucheri Boiss. exracts

Abstract

This study was designed to investigate the antioxidant and
antiproliferative activities of Gypsophila aucheri Boiss.
extracts as well as phenolic content by RP-HPLC
technique. Antioxidant activity potentials of the extracts were evaluated by
four different methods namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and
2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging capacity
tests, cupric ion reducing antioxidant capacity (CUPRAC) method, and metal chelating assay. Antiproliferative activities of the extracts
were tested against breast carcinoma (MCF-7), colorectal carcinoma (HT-29), and
hepatocellular carcinoma (HepG2) cells. RP-HPLC analysis revealed the presence
of eight phenolic compounds in eighteen phenolics scanned. Antioxidant activity
results showed that methanol extract of Gypsophila aucheri displayed more pronounced antioxidant activity than water extract
according to DPPH and ABTS tests, whereas water extract was superior than
methanol extract for CUPRAC and metal chelating activity tests. Cytotoxicity tests demostrated the
promising antiproliferative activity of methanol extract against all the tested
cancer cell lines with its lower IC₅₀ values. However, both extracts
were observed to display moderate biological activity compared to positive
controls. The obtained data suggest that Gypsophila aucheri could
be evaluated as a promising source for food and nutraceutical industries due to
its striking antioxidant and moderate antiproliferative potentials together
with high phytochemical profile

Keywords

Antiproliferative activity, Antioxidant activity, Cell Culture, Gypsophila aucheri Boiss., Phenolic compounds

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