Türkiye'de Geranium Macrorrhizum L. (Geraniaceae) Metanol Ekstresinin Biyolojik ve Antimikrobiyal Aktivitesinin Belirlenmesi

Year 2019, Volume: 12 Issue: 1, 241 - 252, 24.03.2019

Yasemin Karafakıoglu

https://doi.org/10.18185/erzifbed.443982

Abstract

 Dünyanın değişik bölgelerinde
Geranium türleri, ağrı kesici, kan dindirici, antihemoroidal, antidiyabetik,
ateş düşürücü, antidiyareik, kan temizleyici ve gastrointestinal sistem
rahatsızlıklarının tedavisinde kullanılmaktadır. Bu çalışma da Balıkesir ili’nden
toplanan Geranium macrorrhizum türünün
metanol ekstresinde biyolojik ve antimikrobiyal aktivite çalışmaları
yapılmıştır.

“Geranium macrorrhizum metanol ekstresinde”, numunenin toplam fenolik
içerik miktarı 130.18 µg GAE / 1g kuru örnek olarak bulunmuştur. Geranium macrorrhizum metanol ekstresinde,
toplam flavonoid içerik miktarı 55,56 ± 1,20 mg QE/g kuru numune olarak
belirlenmiştir. Geranium macrorrhizum
(Geraniaceae)’nın metanol ekstresinde ABTS radikal giderme aktivitesi 5.80 ±
0,55 lC50 (μg/mL) olarak bulunmuştur. DPPH radikali olarak antioksidan aktivitesi 10,05
μg/mL olarak belirlenmiştir. Geranium
macrorrhizum metanol ekstresi için en duyarlı bakterinin Klebsiella
pneumoniae ATCC700603 (13 mm) olduğu tespit edilmiştir. Elde edilen bulgular Geranium macrorrhizum bitkisinin tıbbi
bitki olarak değerlendirilebileceğini göstermektedir.

İleri
araştırmalarda G. macrorrhizum’un
içerdiği kimyasal bileşenlerin belirlenmesinin yanı sıra in situ ve in vivo testler ile etki mekanizmalarının daha detaylı
araştırılmasının gerekli olduğu sonucuna varılmıştır.

Keywords

Geranium macrorrhizum, Geraniaceae, Toplam Fenolik İçerik

References

• Aedo, C., Garmendia, F. M., Pando, F. (1998). “World Checklist of Geranium L. (Geraniaceae)”. Anales del Jardin Botanico de Madrid, 56(2), 211-252.
• Alanis, A. D., Calzada, F., Cervantes, J. A., Torres, J., Ceballos, G. M. (2005). “Antibacterial Properties of Some Plants Used In Mexican Traditional Medicine For The Treatment Of Gastrointestinal Disorders”, Journal of Ethnopharmacology, 100, 153-157.
• Baba, S.A., Malik, S. A. (2015). “Determination Of Total Phenolic And Flavonoid Content, Antimicrobial And Antioxidant Activity Of A Root Extract Of Arisaema Jacquemontii Blume”,Journal of Taibah University for Science, 9, 449–454.
• Bara, A., Norouzi-Arasi, H., Sedaghat-Sharehjini, S., Baldovini, N. (2006). “Volatile Constituents of Geranium tuberosum L. From Iran”, Natural Product Communications, 1 (5), 387-390.
• Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M. (1966). “Antibiotic Susceptibility Testing By A Standardized Single Disk Method”, Am J Clin Pathol., 45(4):493–496.
• Baytop, T., (1994). Türkçe Bitki Adları Sözlüğü, Ankara: Türk Dil Kurumu Yayınları, No:578.
• Baytop, T., (1999). Türkiye’ de Bitkilerle Tedavi 2. Baskı, İstanbul: Nobel Tıp Kitabevi.
• Calzada, F., Cerda-Garcia-Rojas, C. M., Meckes, M., Cedillo-Rivera, R., Bye R., Mata, R., (1999). “Geranins A and B, New Antiprotozoal A-Type Proanthocyanidins From Geranium Niveum”, Journal of Natural Products, 62, 705- 709.
• Chang, C. ,Yang, M., Wen, H., Chern, J., (2002). “Estimation Of Total Flavonoid Content In Propolis By Two Complementary Colorimetric Methods”. J. Food Drug Anal.,10 pp. 178-182.
• Ghasemzadeh, A., Ghasemzadeh N. (2011). Flavonoids And Phenolic Acids: Role And Biochemical Activity In Plants And Human, Journal of Medicinal Plants Research Vol. 5(31), pp. 6697-6703.
• Greenway, F.L., Frome, B.M., Engels, T.M., McLellan, A., (2003). Temporary Relief of Postherpetic Neuralgia Pain With Topical Geranium Oil, Am J Med.,115(7):586-587.
• Guo, J., Wang, S., Li, X. Zhu, T. (1987). “Antibacterial Constituents Of Geranium Sibiricum L.” Yaoxue Xuebao, 22(1), 28-32.
• Güner, A. (2000). Geranium L. Güner A., Özhatay N., Ekim T., Başer K. H. C.(ed.) Flora of Turkey and East Aegean Islands Supll. II. (s. 104-105). Edinburgh: Edinburgh University Press.
• Ivancheva, S., Zapesochnaya, G., Ognyanov, I., (1976). Flavonoids And Other Substances In Roots Of Geranium macrorrhizum L. Doklady Bolgarskoi Akademii Nauk, 29(2), 205-8.
• Ivancheva, S., Wolllenweber, E. (1989). “Leaf Exudate Flavonoids In Geranium macrorrhizum and G. lucidum”, Indian Drugs, 27 (3), 167-168.
• Ivancheva, S., Manolova, N., Serkedjieva, J., Dimov, V., Ivanovska, N. (1992). “Polyphenols From Bulgarian Medicinal Plants With Anti-Infectious Activity”, Basic Life Sci, 59,717-728.
• İlçim, A., Behçet, L. (2006). “Geranium Kalenderianum (Geraniaceae), A New Species From Turkey”,Annales Botanici Fennici, 43, 451-455.
• Katalinic, V., Milos, M., Kulisic, T., Jukic, M. (2006). “Screening Of 70 Medicinal Plant Extracts For Antioxidant Capacity And Total Phenols”, Food Chemistry, 94, 550-557.
• Kayser, O., Kolodziej, H., Kimura, Y., Okuda, H., Okuda, T., Arichi, S. (1986). “Studies On The Activities Of Tannins and Related Compounds VIII. Effects of Geraniin, Corilagin, and Ellagic Acid Isolated From Geranii Herba On Arachidonate Metabolism In Leukocytes”, Planta Medica, 4, 337-338. Küpeli, E., Tatlı, I.İ., Akdemir, Z. S., Yeşilada E. (2007). “Estimation Of Antinociceptive And Anti-Inflammatory Activity On Geranium pratense subsp. Finitimum”. Journal of Ethnopharmacology, 114, 234-240.
• Kähkönen, M.P., Hopia, A.I., Heikki, J.V., Rauha, J.P., Pihlaja, K., Kujala, T.S., Heinonen, M. (1999). “Antioxidant Activity Of Plant Extracts Containing Phenolic Compounds”, J. Agric. Food Chem. 47:3954–3962. doi: 10.1021/jf990146l.
• Liu, Q. H., Jeong, J. E., Choi, E. J., Moon, Y. H., Woo, E. R. (2006). “A New Furonofuran Lignan From Geranium Thunbergii SIEB. Et ZUCC” ,Archives of Pharmacal Research, 29 (12), 1109-1113.
• Liyana-Pathirana, C.M., Shahidi, F. (2005). “Antioxidant Activity Of Soft Commercial Soft and Hard Wheat (Triticum aestivum L.) As Affected By Gastric pH Conditions”, J. Agric. Food Chem, 53,2433–2440.
• Mantle, D., Eddeb, F., Pickering, A. (2000). “Comparison Of Relative Antioxidant Activities Of British Medicinal Plant Species In Vitro”, Journal of Ethnopharmacology, 72, 47-51.
• Miliauskas, G., Venskutonis, P. R., Van Beek, T.A. (2004). “Screening Of Radical Scavenging Activity Of Some Medicinal Plant Extracts”, Food Chemistry, 85, 231-237.
• Milinaric, A., Kreft, S., Umek, A. ve Strukelj, B. (2000). “Screening Of Selected Plant Extracts For In Vitro Inhibitory Activity On HIV-1 Reverse Transcriptase (HIV-1 RT)”, Pharmazie, 55, 75-77.
• Nikolova, M., Tsvetkova, R., & Ivancheva, S.(2010). “Evaluation of Antioxidant Activity in Some Geraniacean Species”, Botanica Serbica, 34(2), 123-125.
• Okuda, T., Mori K., Seno, K., Hatano, T. (1979). “Constituents of Geranium Thunbergii SIEB. Et ZUCC. VII. High-Performance Reversed-Phase Liquid Chromatography of Hydrolysable Tannins and Related Polyphenols”, Journal of Chromatography, 171, 313-320.
• Okuda, T., Hatano, T., Yazaki, K. (1982). “Dehydrogeraniin, furosinin and furosin, dehydroellagitannins from Geranium thunbergii”, Chemical and Pharmaceutical Bulletin, 30 (3), 1113-1116.
• Öztürk, S. (2008). “Geranium Türlerinde Fitoterapötik Çalışmalar”,Yüksek Lisans Tezi, Gazi Ü. Sağlık Bilimleri Enst.
• Pedro, L., Pais, M.S.S., Scheffer, J.J.C. (1992). “Composition of the essential oil of Geranium robertianum L.” Flavour and Fragrance Journal, 7,223-226.
• Peter, K.V. (2004). “Geranium”. Handbook of Herbs and Spices. Cambridge: Woodhead Publishing.
• Pokharel, Y. R., Liu, Q. H., Aryal, D. K., Kim, Y. G., Woo , E.R. ve Kang, K. W. (2007). “7, 7-Dihydroxy bursehernin inhibits the expression of inductible nitric oxide synthase through NF-ĸB DNA binding suppression”. Nitric Oxide, 16, 274-285.
• Radulović N.S., Stojković, M.B., Mitić, S.S., Randjelović, P.J., Ilić, I.R., Stojanović, N.M., Stojanović-Radić, Z,Z. (2012). “Exploitation of the Antioxidant Potential of Geranium macrorrhizum (Geraniaceae): Hepatoprotective and Antimicrobial Activities”, Nat Prod Commun. 7(12),1609-14.
• Re, R., Pellegrini, N., Protrggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). “Antioxidant activity applying an improved ABTS radical cation decolorization assay”, Free Radical Biology and Medicine, 26,1231-1237.
• Serkedjieva, J. (1997). “Antiinfective Activity of A Plant Preparation From Geranium Sanguineum L.” Pharmazie, 52(10),799-802.
• Serkedjieva, J., Roeva, I., Ivonova, I. (2005). “Combined Anti-influenza Virus Effect of Synthetic and Natural Viral Inhibitors”, Biotechnology& Biotechnology, Virology Supplement, 6-10.
• Sharopov, F., Ahmed, M., Satyal, P., Setzer, W. N., Wink, M. (2017). “Antioxidant Activity and Cytotoxicity of Methanol Extracts of Geranium macrorrhizum and Chemical Composition of its Essential Oil”, Journal of Medicinally Active Plants, 5(2), 53-58.
• Tatlı, İ.İ. (1999). “Geranium Pratense Subsp. Finitimum (Woronow) Knuth Üzerinde Farmakognozik Arastırmalar”, Bilim Uzmanlığı Tezi, Ankara: Hacettepe Üniversitesi.
• Uzun, E., Sariyar, G., Adsersen, A., Karakoc, B., Otuk, G., Oktayoglu, E., Pirildar, S. (2004). “Traditional Medicine in Sakarya Province (Turkey) and Antimicrobial Activities of Selected Species”,J Ethnopharmacol,95(2-3),287-296.
• Velazquez, C., Calzada, F., Torris, J., Gonzalez, F., Ceballos, G. (2006). “Antisecrotary Activity of Plant Used to Treat Gastrointestinal Disorders”, Journal of Ethnopharmacology, 103, 66-70.
• Zaidan, MRS., Noor Rain, A., Badrul, A.R., Adlin, A., Norazah, A., Zakiah, I. (2005). “In vitro Screening of Five Local Medicinal Plants for Antibacterial Activity Using Disc Diffusion Method”, Tropical Biomedicine, 22(2),165–170.